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- W2143804872 abstract "The T-cell receptor (TCR) is the critical structure involved in antigen recognition of T lymphocytes. Although the pig has a large proportion of circulating T lymphocytes bearing the γδ TCR, their study has been impeded due to the lack of specific antibodies. Here a monoclonal antibody (mAb) PPT27 directed to γδ TCR is described. Flow cytometry analyses showed that the mAb recognized a subset of T lymphocytes of which the majority expressed no CD2, CD4 and CD8 whilst the minority bore CD2 and CD8. The mAb precipitated a protein of 86 000 MW under non-reducing conditions and a doublet of 43 000 MW under reducing conditions from peripheral blood T lymphocytes lysed in nonidet P-40 buffer, whilst it precipitated the CD3–TCR complex from the cells lysed in digitonin. Further analysis revealed that the antibody recognized the majority, but not all, of the γδ T cells, suggesting that there may be more isotypes of γδ TCR than currently believed. The antibody was unable to stimulate γδ T cells to proliferate in vitro, suggesting that these cells are activated by a different activation mechanism from that of αβ T cells." @default.
- W2143804872 created "2016-06-24" @default.
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- W2143804872 date "2000-04-01" @default.
- W2143804872 modified "2023-10-15" @default.
- W2143804872 title "Characterization of the porcine γδ T-cell receptor structure and cellular distribution by monoclonal antibody PPT27" @default.
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- W2143804872 doi "https://doi.org/10.1046/j.1365-2567.2000.00019.x" @default.
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