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- W2143804879 abstract "We investigated the cellular events associated with cell death in the coleoptile of rice plants ( Oryza sativa L.). Seeds germinated under submergence produced coleoptiles that were more elongated than those grown under aerobic conditions. Transfer of seedlings to aerobic conditions was associated with coleoptile opening (i.e. splitting) due to death of specific cells in the side of the organ. Another type of cell death occurred in the formation of lysigenous aerenchyma. Senescence of the coleoptile was also noted, during which discolouration of the chlorophyll and tissue browning were apparent. DNA fragmentation was observed by deoxynucleotidyltransferase-mediated dUTP nick end labelling (TUNEL) assay, and further confirmed by the appearance of oligonucleosomal DNA ladders in senescent coleoptile cells. Two nucleases (Nuc-a and Nuc-b) were detected by in-gel-assay from proteins isolated from coleoptiles. Nuc-a, commonly observed in three cell death phases required either Ca 2+ or Mg 2+ , whereas Nuc-b which appeared during senescence required both Ca 2+ and Mg 2+ . Both nucleases were strongly inhibited by Zn 2+ . Copyright 2000 Annals of Botany Company" @default.
- W2143804879 created "2016-06-24" @default.
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- W2143804879 date "2000-08-01" @default.
- W2143804879 modified "2023-10-02" @default.
- W2143804879 title "Coleoptile Senescence in Rice (Oryza sativa L.)" @default.
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- W2143804879 doi "https://doi.org/10.1006/anbo.2000.1199" @default.
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