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• W2144028852 endingPage "197" @default.
• W2144028852 startingPage "191" @default.
• W2144028852 abstract "We constructed internal controls (ICs) to provide assurance that clinical specimens are successfully amplified and detected. The IC nucleic acids contain primer binding regions identical to those of the target sequence and contain a unique probe binding region that differentiates the IC from amplified target nucleic acid. Because only 20 copies of the IC are introduced into each test sample, a positive IC signal indicates that amplification was sufficient to generate a positive signal from targets present at the limit of test sensitivity. The COBAS AMPLICOR Chlamydia trachomatis, Neisseria gonorrhoeae, Mycobacterium tuberculosis, and human hepatitis C virus tests exhibited inhibition rates ranging from 5 to 9%. Approximately 64% of these inhibitory specimens were not inhibitory when a second aliquot was tested. Because repeatedly inhibitory specimens were not reported as false negative and because additional infected specimens were detected during retesting, test sensitivities were 1 to 6% greater than they would have been if the IC had not been used." @default.
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• W2144028852 date "1998-01-01" @default.
• W2144028852 modified "2023-10-10" @default.
• W2144028852 title "An Internal Control for Routine Diagnostic PCR: Design, Properties, and Effect on Clinical Performance" @default.
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• W2144028852 doi "https://doi.org/10.1128/jcm.36.1.191-197.1998" @default.
• W2144028852 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/124832" @default.
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