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• W2144046875 abstract "Background aims A robust methodology for the isolation of cord blood-derived multipotent mesenchymal stromal cells (CB-MSCs) from fresh umbilical cord blood has not been reported in any species. The objective of this study was to improve the isolation procedure for equine CB-MSCs. Methods Pre-culture separation of red and white blood cells was done using either PrepaCyte?-EQ medium or Ficoll-Paque? PREMIUM density medium. Regular FBS and MSC-qualified FBS were compared for their ability to support the establishment of putative primary MSC colonies. Results and conclusions Our results indicate that PrepaCyte®-EQ medium is superior to Ficoll-Paque™ PREMIUM density medium for the isolation of putative equine CB MSC and that MSC-qualified FBS may improve the isolation efficiency. A robust methodology for the isolation of cord blood-derived multipotent mesenchymal stromal cells (CB-MSCs) from fresh umbilical cord blood has not been reported in any species. The objective of this study was to improve the isolation procedure for equine CB-MSCs. Pre-culture separation of red and white blood cells was done using either PrepaCyte?-EQ medium or Ficoll-Paque? PREMIUM density medium. Regular FBS and MSC-qualified FBS were compared for their ability to support the establishment of putative primary MSC colonies. Our results indicate that PrepaCyte®-EQ medium is superior to Ficoll-Paque™ PREMIUM density medium for the isolation of putative equine CB MSC and that MSC-qualified FBS may improve the isolation efficiency." @default.
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• W2144046875 date "2009-01-01" @default.
• W2144046875 modified "2023-10-18" @default.
• W2144046875 title "Improved isolation protocol for equine cord blood-derived mesenchymal stromal cells" @default.
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• W2144046875 doi "https://doi.org/10.1080/14653240902887259" @default.
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