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• W2144206181 startingPage "1044" @default.
• W2144206181 abstract "We constructed a series of recombinant genomes which directed expression of the enzyme chloramphenicol acetyltransferase (CAT) in mammalian cells. The prototype recombinant in this series, pSV2-cat, consisted of the beta-lactamase gene and origin of replication from pBR322 coupled to a simian virus 40 (SV40) early transcription region into which CAT coding sequences were inserted. Readily measured levels of CAT accumulated within 48 h after the introduction of pSV2-cat DNA into African green monkey kidney CV-1 cells. Because endogenous CAT activity is not present in CV-1 or other mammalian cells, and because rapid, sensitive assays for CAT activity are available, these recombinants provided a uniquely convenient system for monitoring the expression of foreign DNAs in tissue culture cells. To demonstrate the usefulness of this system, we constructed derivatives of pSV2-cat from which part or all of the SV40 promoter region was removed. Deletion of one copy of the 72-base-pair repeat sequence in the SV40 promoter caused no significant decrease in CAT synthesis in monkey kidney CV-1 cells; however, an additional deletion of 50 base pairs from the second copy of the repeats reduced CAT synthesis to 11% of its level in the wild type. We also constructed a recombinant, pSV0-cat, in which the entire SV40 promoter region was removed and a unique HindIII site was substituted for the insertion of other promoter sequences." @default.
• W2144206181 created "2016-06-24" @default.
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• W2144206181 date "1982-09-01" @default.
• W2144206181 modified "2023-10-10" @default.
• W2144206181 title "Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells." @default.
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• W2144206181 doi "https://doi.org/10.1128/mcb.2.9.1044" @default.
• W2144206181 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/369897" @default.
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