FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2144258781> ?p ?o ?g. }

• W2144258781 endingPage "1267" @default.
• W2144258781 startingPage "1258" @default.
• W2144258781 abstract "Abstract —Platelet-derived growth factor (PDGF) is a mitogen and chemoattractant for a wide variety of cell types. The genes encoding PDGF A chain (PDGF-A) and PDGF B chain (PDGF-B) reside on separate chromosomes and are independently regulated at the level of transcription. Regulatory events underlying inducible PDGF-A expression have been the focus of much investigation. However, mechanisms that inhibit transcription of this gene are not well understood. In this study, we report the capacity of a newly cloned DNA binding factor, GC factor 2 (GCF2), to repress expression driven by the human PDGF-A promoter. 5′ Deletion and transient cotransfection analysis in vascular endothelial cells revealed that GCF2 repression is mediated by a nucleotide region located in the proximal region of the PDGF-A promoter. Electrophoretic mobility shift assays demonstrate that GCF2 binds to this region in a specific and dose-dependent manner. Interestingly, the site bound by GCF2 overlaps those for specificity protein-1 (Sp1) and early growth response factor-1 (Egr-1), zinc finger transcription factors that direct basal and inducible expression of the PDGF-A gene. Gel shift experiments revealed that GCF2 competes with these factors for interaction with the PDGF-A promoter. Overexpression of GCF2 suppressed endogenous PDGF-A expression in vascular endothelial cells and smooth muscle cells. GCF2 was induced on mechanical injury of cells in culture as well as after balloon injury of the rat carotid artery wall. Time course studies revealed the sustained induction of GCF2 after injury while PDGF-A levels sharply returned to baseline. Smooth muscle cell proliferation was inhibited by GCF2, an effect reversed by the addition of exogenous PDGF-AA. These findings demonstrate negative regulation of PDGF-A expression by GCF2. This is the first report of the induction of an endogenous transcriptional repressor in the rat vessel wall." @default.
• W2144258781 created "2016-06-24" @default.
• W2144258781 creator A5011903423 @default.
• W2144258781 creator A5012564439 @default.
• W2144258781 creator A5016487477 @default.
• W2144258781 creator A5020097005 @default.
• W2144258781 creator A5058401548 @default.
• W2144258781 creator A5065860849 @default.
• W2144258781 creator A5067667648 @default.
• W2144258781 creator A5086363677 @default.
• W2144258781 date "1999-06-11" @default.
• W2144258781 modified "2023-10-10" @default.
• W2144258781 title "GC Factor 2 Represses Platelet-Derived Growth Factor A-Chain Gene Transcription and Is Itself Induced by Arterial Injury" @default.
• W2144258781 cites W1498565802 @default.
• W2144258781 cites W1570592780 @default.
• W2144258781 cites W1575595760 @default.
• W2144258781 cites W1597241698 @default.
• W2144258781 cites W1966156883 @default.
• W2144258781 cites W1966630774 @default.
• W2144258781 cites W1971155327 @default.
• W2144258781 cites W1972748528 @default.
• W2144258781 cites W1973912514 @default.
• W2144258781 cites W1974609384 @default.
• W2144258781 cites W1979356105 @default.
• W2144258781 cites W1980669305 @default.
• W2144258781 cites W1984583623 @default.
• W2144258781 cites W1986998915 @default.
• W2144258781 cites W1989274470 @default.
• W2144258781 cites W1993487598 @default.
• W2144258781 cites W1995646607 @default.
• W2144258781 cites W1995741957 @default.
• W2144258781 cites W1996674170 @default.
• W2144258781 cites W1998109615 @default.
• W2144258781 cites W2001381510 @default.
• W2144258781 cites W2001870404 @default.
• W2144258781 cites W2006345574 @default.
• W2144258781 cites W2008468198 @default.
• W2144258781 cites W2008647600 @default.
• W2144258781 cites W2010791130 @default.
• W2144258781 cites W2015669817 @default.
• W2144258781 cites W2020427232 @default.
• W2144258781 cites W2030248303 @default.
• W2144258781 cites W2039462089 @default.
• W2144258781 cites W2041456078 @default.
• W2144258781 cites W2043789254 @default.
• W2144258781 cites W2055220950 @default.
• W2144258781 cites W2064444728 @default.
• W2144258781 cites W2069965054 @default.
• W2144258781 cites W2071366179 @default.
• W2144258781 cites W2079743245 @default.
• W2144258781 cites W2096428903 @default.
• W2144258781 cites W2123995229 @default.
• W2144258781 cites W2126540114 @default.
• W2144258781 cites W2151045797 @default.
• W2144258781 cites W300264704 @default.
• W2144258781 cites W88442112 @default.
• W2144258781 doi "https://doi.org/10.1161/01.res.84.11.1258" @default.
• W2144258781 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10364563" @default.
• W2144258781 hasPublicationYear "1999" @default.
• W2144258781 type Work @default.
• W2144258781 sameAs 2144258781 @default.
• W2144258781 citedByCount "54" @default.
• W2144258781 countsByYear W21442587812012 @default.
• W2144258781 countsByYear W21442587812013 @default.
• W2144258781 countsByYear W21442587812014 @default.
• W2144258781 countsByYear W21442587812015 @default.
• W2144258781 countsByYear W21442587812016 @default.
• W2144258781 countsByYear W21442587812017 @default.
• W2144258781 countsByYear W21442587812018 @default.
• W2144258781 countsByYear W21442587812019 @default.
• W2144258781 countsByYear W21442587812021 @default.
• W2144258781 countsByYear W21442587812022 @default.
• W2144258781 countsByYear W21442587812023 @default.
• W2144258781 crossrefType "journal-article" @default.
• W2144258781 hasAuthorship W2144258781A5011903423 @default.
• W2144258781 hasAuthorship W2144258781A5012564439 @default.
• W2144258781 hasAuthorship W2144258781A5016487477 @default.
• W2144258781 hasAuthorship W2144258781A5020097005 @default.
• W2144258781 hasAuthorship W2144258781A5058401548 @default.
• W2144258781 hasAuthorship W2144258781A5065860849 @default.
• W2144258781 hasAuthorship W2144258781A5067667648 @default.
• W2144258781 hasAuthorship W2144258781A5086363677 @default.
• W2144258781 hasBestOaLocation W21442587811 @default.
• W2144258781 hasConcept C101762097 @default.
• W2144258781 hasConcept C104317684 @default.
• W2144258781 hasConcept C134018914 @default.
• W2144258781 hasConcept C150194340 @default.
• W2144258781 hasConcept C153911025 @default.
• W2144258781 hasConcept C170493617 @default.
• W2144258781 hasConcept C180361614 @default.
• W2144258781 hasConcept C24284526 @default.
• W2144258781 hasConcept C2775960820 @default.
• W2144258781 hasConcept C2777124376 @default.
• W2144258781 hasConcept C2779395532 @default.
• W2144258781 hasConcept C2780545995 @default.
• W2144258781 hasConcept C2781294074 @default.
• W2144258781 hasConcept C2992686903 @default.
• W2144258781 hasConcept C54355233 @default.

• next