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- W2144262005 abstract "A methodology that can monitor and identify inorganic elements in biological and environmental systems was developed. Size exclusion chromatography (SEC) separates biomolecules, which are then nebulized by a microconcentric nebulizer. The resulting aerosol is desolved and introduced into either a high resolution ICP-MS device or a quadrupole device with a collision cell. Because of the high sensitivity and spectral resolution and high sample introduction efficiency, many unusual or difficult elements, such as Cr, Se, Cd and U, can be observed at ambient levels bound to proteins in human serum. These measurements are made in only a few minutes without preliminary isolation and preconcentration steps. Serum samples can be titrated with spikes of various elements to determine which proteins bind a given metal and oxidation state. Experiments concerning the effects of breaking disulfide linkages and denaturation on metal binding in proteins were also investigated. Elemental distribution in liver extract was also obtained." @default.
- W2144262005 created "2016-06-24" @default.
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- W2144262005 date "1999-11-08" @default.
- W2144262005 modified "2023-09-26" @default.
- W2144262005 title "Elemental speciation in biomolecules by LC-ICP-MS with magnetic sector and collision cell instruments" @default.
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- W2144262005 doi "https://doi.org/10.2172/754790" @default.
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