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- W2144340771 abstract "An enzyme system which converts anthranilic acid to catechol was detected in the leaves of Tecoma stans, and its properties studied. The system is present exclusively in the chloroplast fraction of the leaves. The optimum pH of the reaction is 5·2 and maximum activity was obtained with citrate-phosphate buffer. There was good stoichiometry between the amounts of anthranilic acid disappeared and the amounts of catechol and ammonia formed. The enzyme system showed an absolute requirement for oxygen and evidence was obtained for the probable participation of NADPH and FAD in the hydroxylation step. The optimum concentration of anthranilic acid was 10−4 M; at higher concentrations the reaction was inhibited to a considerable extent. Cyanide, pyrophosphate, and EDTA also caused inhibition indicating a requirement for metal ions." @default.
- W2144340771 created "2016-06-24" @default.
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- W2144340771 date "1964-03-01" @default.
- W2144340771 modified "2023-09-27" @default.
- W2144340771 title "Enzymatic conversion of anthranilic acid to catechol by chloroplast from the leaves of Tecoma stans" @default.
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- W2144340771 doi "https://doi.org/10.1016/s0031-9422(00)88044-9" @default.
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