FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2144385102> ?p ?o ?g. }

• W2144385102 endingPage "631" @default.
• W2144385102 startingPage "619" @default.
• W2144385102 abstract "Combined patch-clamp and Fura-2 measurements were performed on chinese hamster ovary (CHO) cells co-expressing two channel proteins involved in skeletal muscle excitation-contraction (E-C) coupling, the ryanodine receptor (RyR)-Ca2+ release channel (in the membrane of internal Ca2+ stores) and the dihydropyridine receptor (DHPR)-Ca2+ channel (in the plasma membrane). To ensure expression of functional L-type Ca+ channels, we expressed alpha2, beta, and gamma DHPR subunits and a chimeric DHPR alpha(i) subunit in which the putative cytoplasmic loop between repeats II and III is of skeletal origin and the remainder is cardiac. There was no clear indication of skeletal-type coupling between the DHPR and the RyR; depolarization failed to induce a Ca2+ transient (CaT) in the absence of extracellular Ca2+ ([Ca2+]o). However, in the presence of [Ca2+]o, depolarization evoked CaTs with a bell-shaped voltage dependence. About 30% of the cells tested exhibited two kinetic components: a fast transient increase in intracellular Ca2+ concentration ([Ca2+]i) (the first component; reaching 95% of its peak <0.6 s after depolarization) followed by a second increase in [Ca2+]i which lasted for 5-10 s (the second component). Our results suggest that the first component primarily reflected Ca2+ influx through Ca2+ channels, whereas the second component resulted from Ca2+ release through the RyR expressed in the membrane of internal Ca2+ stores. However, the onset and the rate of Ca2+ release appeared to be much slower than in native cardiac myocytes, despite a similar activation rate of Ca2+ current. These results suggest that the skeletal muscle RyR isoform supports Ca2+-induced Ca2+ release but that the distance between the DHPRs and the RyRs is, on average, much larger in the cotransfected CHO cells than in cardiac myocytes. We conclude that morphological properties of T-tubules and/or proteins other than the DHPR and the RyR are required for functional close coupling like that observed in skeletal or cardiac muscle. Nevertheless, some of our results imply that these two channels are potentially able to directly interact with each other." @default.
• W2144385102 created "2016-06-24" @default.
• W2144385102 creator A5012247108 @default.
• W2144385102 creator A5024358171 @default.
• W2144385102 creator A5045126391 @default.
• W2144385102 creator A5058943509 @default.
• W2144385102 creator A5062939633 @default.
• W2144385102 creator A5064410327 @default.
• W2144385102 creator A5071870855 @default.
• W2144385102 creator A5076536086 @default.
• W2144385102 date "1997-05-01" @default.
• W2144385102 modified "2023-10-01" @default.
• W2144385102 title "Ca2+-induced Ca2+ Release in Chinese Hamster Ovary (CHO) Cells Co-expressing Dihydropyridine and Ryanodine Receptors" @default.
• W2144385102 cites W1963851776 @default.
• W2144385102 cites W1968408785 @default.
• W2144385102 cites W1971033431 @default.
• W2144385102 cites W1971311671 @default.
• W2144385102 cites W1974143212 @default.
• W2144385102 cites W1994051052 @default.
• W2144385102 cites W1997050207 @default.
• W2144385102 cites W1998299402 @default.
• W2144385102 cites W2011038097 @default.
• W2144385102 cites W2021647551 @default.
• W2144385102 cites W2024929666 @default.
• W2144385102 cites W2027539568 @default.
• W2144385102 cites W2028165798 @default.
• W2144385102 cites W2035885166 @default.
• W2144385102 cites W2039547987 @default.
• W2144385102 cites W2040097492 @default.
• W2144385102 cites W2042224433 @default.
• W2144385102 cites W2051473926 @default.
• W2144385102 cites W2062157267 @default.
• W2144385102 cites W2063081959 @default.
• W2144385102 cites W2069230499 @default.
• W2144385102 cites W2071724691 @default.
• W2144385102 cites W2071850038 @default.
• W2144385102 cites W2078360969 @default.
• W2144385102 cites W2083310303 @default.
• W2144385102 cites W2083812366 @default.
• W2144385102 cites W2087651417 @default.
• W2144385102 cites W2089596501 @default.
• W2144385102 cites W2090803944 @default.
• W2144385102 cites W2094985269 @default.
• W2144385102 cites W2117466810 @default.
• W2144385102 cites W2126674741 @default.
• W2144385102 cites W2131310568 @default.
• W2144385102 cites W2134064208 @default.
• W2144385102 cites W2138450406 @default.
• W2144385102 cites W2145287037 @default.
• W2144385102 cites W2145484503 @default.
• W2144385102 cites W2145758853 @default.
• W2144385102 cites W2153194642 @default.
• W2144385102 cites W2155354600 @default.
• W2144385102 cites W2161957047 @default.
• W2144385102 cites W2170657110 @default.
• W2144385102 cites W4239219601 @default.
• W2144385102 doi "https://doi.org/10.1085/jgp.109.5.619" @default.
• W2144385102 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2217062" @default.
• W2144385102 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9154908" @default.
• W2144385102 hasPublicationYear "1997" @default.
• W2144385102 type Work @default.
• W2144385102 sameAs 2144385102 @default.
• W2144385102 citedByCount "23" @default.
• W2144385102 countsByYear W21443851022012 @default.
• W2144385102 countsByYear W21443851022017 @default.
• W2144385102 crossrefType "journal-article" @default.
• W2144385102 hasAuthorship W2144385102A5012247108 @default.
• W2144385102 hasAuthorship W2144385102A5024358171 @default.
• W2144385102 hasAuthorship W2144385102A5045126391 @default.
• W2144385102 hasAuthorship W2144385102A5058943509 @default.
• W2144385102 hasAuthorship W2144385102A5062939633 @default.
• W2144385102 hasAuthorship W2144385102A5064410327 @default.
• W2144385102 hasAuthorship W2144385102A5071870855 @default.
• W2144385102 hasAuthorship W2144385102A5076536086 @default.
• W2144385102 hasBestOaLocation W21443851021 @default.
• W2144385102 hasConcept C113217602 @default.
• W2144385102 hasConcept C12554922 @default.
• W2144385102 hasConcept C126322002 @default.
• W2144385102 hasConcept C147944092 @default.
• W2144385102 hasConcept C170493617 @default.
• W2144385102 hasConcept C175656101 @default.
• W2144385102 hasConcept C178790620 @default.
• W2144385102 hasConcept C181911157 @default.
• W2144385102 hasConcept C185592680 @default.
• W2144385102 hasConcept C18699975 @default.
• W2144385102 hasConcept C2781212610 @default.
• W2144385102 hasConcept C28406088 @default.
• W2144385102 hasConcept C4141045 @default.
• W2144385102 hasConcept C519063684 @default.
• W2144385102 hasConcept C55493867 @default.
• W2144385102 hasConcept C71924100 @default.
• W2144385102 hasConcept C79879829 @default.
• W2144385102 hasConcept C86803240 @default.
• W2144385102 hasConceptScore W2144385102C113217602 @default.
• W2144385102 hasConceptScore W2144385102C12554922 @default.
• W2144385102 hasConceptScore W2144385102C126322002 @default.
• W2144385102 hasConceptScore W2144385102C147944092 @default.
• W2144385102 hasConceptScore W2144385102C170493617 @default.

• next