FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2144424972> ?p ?o ?g. }

• W2144424972 endingPage "2033" @default.
• W2144424972 startingPage "2025" @default.
• W2144424972 abstract "A set of polymer carriers for DNA delivery was synthesized by combining monodisperse, sequence-defined poly(amidoamine) (PAA) segments with poly(ethylene oxide) (PEO) blocks. The precise definition of the PAA segments provides the possibility of correlating the chemical structure (monomer sequence) with the resulting biological properties. Three different PAA-PEO conjugates were synthesized by solid-phase supported synthesis, and the cationic nature of the PAA segments was systematically varied. This allows for the tailoring of interactions with double-stranded plasmid DNA (dsDNA). The potential of the PAA-PEO conjugates as non-viral vectors for gene delivery is demonstrated by investigating the dsDNA complexation and condensation properties. Depending on the applied carrier, a transition in polyplex (polymer-DNA ion complex) structures is observed. This reaches from extended ring-like structures to highly compact toroidal structures, where supercoiling of the DNA is induced. An aggregation model is proposed that is based on structural investigations of the polyplexes with atomic force microscopy (AFM) and dynamic light scattering (DLS). While the cationic PAA segment mediates primarily the contact of the carrier to the dsDNA, the PEO block stabilizes the polyplex and generates a stealth aggregate, as was suggested by Zeta potentials that were close to zero. The controlled aggregation leads to stable, single-plasmid complexes, and stabilizes the DNA structure itself. This is shown by ethidium bromide intercalation assays and DNase digestion assays. The presented PAA-PEO systems allow for the formation of well-defined single-plasmid polyplexes, preventing hard DNA compression and strongly polydisperse polyplexes. Moreover carrier polymers and the resulting polyplexes exhibit no cytotoxicity, as was shown by viability tests; this makes the carriers potentially suitable for in vivo delivery applications." @default.
• W2144424972 created "2016-06-24" @default.
• W2144424972 creator A5002972085 @default.
• W2144424972 creator A5046751755 @default.
• W2144424972 creator A5071503658 @default.
• W2144424972 creator A5073720658 @default.
• W2144424972 creator A5075963769 @default.
• W2144424972 date "2008-02-27" @default.
• W2144424972 modified "2023-09-27" @default.
• W2144424972 title "Tailor-Made Poly(amidoamine)s for Controlled Complexation and Condensation of DNA" @default.
• W2144424972 cites W1503734031 @default.
• W2144424972 cites W1965800158 @default.
• W2144424972 cites W1968640798 @default.
• W2144424972 cites W1975081121 @default.
• W2144424972 cites W1977238598 @default.
• W2144424972 cites W1982299508 @default.
• W2144424972 cites W1987583261 @default.
• W2144424972 cites W1997993853 @default.
• W2144424972 cites W1998118881 @default.
• W2144424972 cites W2001578546 @default.
• W2144424972 cites W2003414091 @default.
• W2144424972 cites W2005247635 @default.
• W2144424972 cites W2007002347 @default.
• W2144424972 cites W2009508594 @default.
• W2144424972 cites W2010655741 @default.
• W2144424972 cites W2013168545 @default.
• W2144424972 cites W2014657913 @default.
• W2144424972 cites W2016568056 @default.
• W2144424972 cites W2022095799 @default.
• W2144424972 cites W2022234371 @default.
• W2144424972 cites W2023104469 @default.
• W2144424972 cites W2024173945 @default.
• W2144424972 cites W2024526439 @default.
• W2144424972 cites W2028120444 @default.
• W2144424972 cites W2029038878 @default.
• W2144424972 cites W2030855909 @default.
• W2144424972 cites W2032689379 @default.
• W2144424972 cites W2043764483 @default.
• W2144424972 cites W2058234065 @default.
• W2144424972 cites W2059884965 @default.
• W2144424972 cites W2070794824 @default.
• W2144424972 cites W2074254849 @default.
• W2144424972 cites W2087497808 @default.
• W2144424972 cites W2087589993 @default.
• W2144424972 cites W2100751439 @default.
• W2144424972 cites W2105470560 @default.
• W2144424972 cites W2123097988 @default.
• W2144424972 cites W2142987628 @default.
• W2144424972 cites W2152179330 @default.
• W2144424972 cites W2154028926 @default.
• W2144424972 cites W2160468918 @default.
• W2144424972 cites W2163792954 @default.
• W2144424972 cites W2163921171 @default.
• W2144424972 cites W4207061948 @default.
• W2144424972 cites W4233309778 @default.
• W2144424972 cites W4237233616 @default.
• W2144424972 cites W4242422889 @default.
• W2144424972 cites W4243024275 @default.
• W2144424972 cites W95741617 @default.
• W2144424972 doi "https://doi.org/10.1002/chem.200701223" @default.
• W2144424972 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18260067" @default.
• W2144424972 hasPublicationYear "2008" @default.
• W2144424972 type Work @default.
• W2144424972 sameAs 2144424972 @default.
• W2144424972 citedByCount "90" @default.
• W2144424972 countsByYear W21444249722012 @default.
• W2144424972 countsByYear W21444249722013 @default.
• W2144424972 countsByYear W21444249722014 @default.
• W2144424972 countsByYear W21444249722015 @default.
• W2144424972 countsByYear W21444249722016 @default.
• W2144424972 countsByYear W21444249722017 @default.
• W2144424972 countsByYear W21444249722018 @default.
• W2144424972 countsByYear W21444249722019 @default.
• W2144424972 countsByYear W21444249722020 @default.
• W2144424972 countsByYear W21444249722021 @default.
• W2144424972 countsByYear W21444249722022 @default.
• W2144424972 countsByYear W21444249722023 @default.
• W2144424972 crossrefType "journal-article" @default.
• W2144424972 hasAuthorship W2144424972A5002972085 @default.
• W2144424972 hasAuthorship W2144424972A5046751755 @default.
• W2144424972 hasAuthorship W2144424972A5071503658 @default.
• W2144424972 hasAuthorship W2144424972A5073720658 @default.
• W2144424972 hasAuthorship W2144424972A5075963769 @default.
• W2144424972 hasConcept C104317684 @default.
• W2144424972 hasConcept C11432220 @default.
• W2144424972 hasConcept C115028873 @default.
• W2144424972 hasConcept C12554922 @default.
• W2144424972 hasConcept C135983454 @default.
• W2144424972 hasConcept C137824038 @default.
• W2144424972 hasConcept C14631669 @default.
• W2144424972 hasConcept C153356533 @default.
• W2144424972 hasConcept C155672457 @default.
• W2144424972 hasConcept C166940927 @default.
• W2144424972 hasConcept C171250308 @default.
• W2144424972 hasConcept C178790620 @default.
• W2144424972 hasConcept C183882617 @default.
• W2144424972 hasConcept C185592680 @default.
• W2144424972 hasConcept C188027245 @default.

• next