FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2144509484> ?p ?o ?g. }

• W2144509484 endingPage "367" @default.
• W2144509484 startingPage "357" @default.
• W2144509484 abstract "SerpinA12 (vaspin) is thought to be mainly expressed in adipose tissue and has multiple beneficial effects on metabolic, inflammatory and atherogenic processes related to obesity. KLK7 (kallikrein 7) is the only known protease target of vaspin to date and is inhibited with a moderate inhibition rate. In the crystal structure, the cleavage site (P1-P1') of the vaspin reactive centre loop is fairly rigid compared with the flexible residues before P2, possibly supported by an ionic interaction of P1' glutamate (Glu(379)) with an arginine residue (Arg(302)) of the β-sheet C. A P1' glutamate seems highly unusual and unfavourable for the protease KLK7. We characterized vaspin mutants to investigate the roles of these two residues in protease inhibition and recognition by vaspin. Reactive centre loop mutations changing the P1' residue or altering the reactive centre loop conformation significantly increased inhibition parameters, whereas removal of the positive charge within β-sheet C impeded the serpin-protease interaction. Arg(302) is a crucial contact to enable vaspin recognition by KLK7 and it supports moderate inhibition of the serpin despite the presence of the detrimental P1' Glu(379), which clearly represents a major limiting factor for vaspin-inhibitory activity. We also show that the vaspin-inhibition rate for KLK7 can be modestly increased by heparin and demonstrate that vaspin is a heparin-binding serpin. Noteworthily, we observed vaspin as a remarkably thermostable serpin and found that Glu(379) and Arg(302) influence heat-induced polymerization. These structural and functional results reveal the mechanistic basis of how reactive centre loop sequence and exosite interaction in vaspin enable KLK7 recognition and regulate protease inhibition as well as stability of this adipose tissue-derived serpin." @default.
• W2144509484 created "2016-06-24" @default.
• W2144509484 creator A5002335336 @default.
• W2144509484 creator A5011853443 @default.
• W2144509484 creator A5036790593 @default.
• W2144509484 creator A5056855194 @default.
• W2144509484 creator A5061278872 @default.
• W2144509484 creator A5077558649 @default.
• W2144509484 date "2015-09-04" @default.
• W2144509484 modified "2023-10-17" @default.
• W2144509484 title "A unique serpin P1′ glutamate and a conserved β-sheet C arginine are key residues for activity, protease recognition and stability of serpinA12 (vaspin)" @default.
• W2144509484 cites W1517209311 @default.
• W2144509484 cites W1568801985 @default.
• W2144509484 cites W1588568089 @default.
• W2144509484 cites W1969543928 @default.
• W2144509484 cites W1980660976 @default.
• W2144509484 cites W1988162578 @default.
• W2144509484 cites W2001641653 @default.
• W2144509484 cites W2009420807 @default.
• W2144509484 cites W2011955429 @default.
• W2144509484 cites W2012508243 @default.
• W2144509484 cites W2014952978 @default.
• W2144509484 cites W2017400542 @default.
• W2144509484 cites W2024273754 @default.
• W2144509484 cites W2027551556 @default.
• W2144509484 cites W2032615313 @default.
• W2144509484 cites W2038840577 @default.
• W2144509484 cites W2039507693 @default.
• W2144509484 cites W2039873895 @default.
• W2144509484 cites W2046430783 @default.
• W2144509484 cites W2048467423 @default.
• W2144509484 cites W2049271565 @default.
• W2144509484 cites W2058389174 @default.
• W2144509484 cites W2061346634 @default.
• W2144509484 cites W2067921100 @default.
• W2144509484 cites W2083120544 @default.
• W2144509484 cites W2086487704 @default.
• W2144509484 cites W2091305296 @default.
• W2144509484 cites W2092473594 @default.
• W2144509484 cites W2094820287 @default.
• W2144509484 cites W2094916717 @default.
• W2144509484 cites W2099105421 @default.
• W2144509484 cites W2105506930 @default.
• W2144509484 cites W2106290432 @default.
• W2144509484 cites W2110808180 @default.
• W2144509484 cites W2113244288 @default.
• W2144509484 cites W2116221091 @default.
• W2144509484 cites W2122377543 @default.
• W2144509484 cites W2123587152 @default.
• W2144509484 cites W2124026197 @default.
• W2144509484 cites W2130479394 @default.
• W2144509484 cites W2133604915 @default.
• W2144509484 cites W2154714625 @default.
• W2144509484 cites W2158288207 @default.
• W2144509484 cites W2159211495 @default.
• W2144509484 cites W2161432223 @default.
• W2144509484 cites W2162244749 @default.
• W2144509484 cites W2180229411 @default.
• W2144509484 cites W2322425412 @default.
• W2144509484 cites W4248872320 @default.
• W2144509484 doi "https://doi.org/10.1042/bj20150643" @default.
• W2144509484 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26199422" @default.
• W2144509484 hasPublicationYear "2015" @default.
• W2144509484 type Work @default.
• W2144509484 sameAs 2144509484 @default.
• W2144509484 citedByCount "14" @default.
• W2144509484 countsByYear W21445094842015 @default.
• W2144509484 countsByYear W21445094842017 @default.
• W2144509484 countsByYear W21445094842018 @default.
• W2144509484 countsByYear W21445094842020 @default.
• W2144509484 countsByYear W21445094842021 @default.
• W2144509484 countsByYear W21445094842022 @default.
• W2144509484 countsByYear W21445094842023 @default.
• W2144509484 crossrefType "journal-article" @default.
• W2144509484 hasAuthorship W2144509484A5002335336 @default.
• W2144509484 hasAuthorship W2144509484A5011853443 @default.
• W2144509484 hasAuthorship W2144509484A5036790593 @default.
• W2144509484 hasAuthorship W2144509484A5056855194 @default.
• W2144509484 hasAuthorship W2144509484A5061278872 @default.
• W2144509484 hasAuthorship W2144509484A5077558649 @default.
• W2144509484 hasBestOaLocation W21445094842 @default.
• W2144509484 hasConcept C104317684 @default.
• W2144509484 hasConcept C170493617 @default.
• W2144509484 hasConcept C181199279 @default.
• W2144509484 hasConcept C182220744 @default.
• W2144509484 hasConcept C185592680 @default.
• W2144509484 hasConcept C2776714187 @default.
• W2144509484 hasConcept C2778965386 @default.
• W2144509484 hasConcept C2781338088 @default.
• W2144509484 hasConcept C52853521 @default.
• W2144509484 hasConcept C55493867 @default.
• W2144509484 hasConcept C61174792 @default.
• W2144509484 hasConcept C86803240 @default.
• W2144509484 hasConceptScore W2144509484C104317684 @default.
• W2144509484 hasConceptScore W2144509484C170493617 @default.
• W2144509484 hasConceptScore W2144509484C181199279 @default.
• W2144509484 hasConceptScore W2144509484C182220744 @default.
• W2144509484 hasConceptScore W2144509484C185592680 @default.

• next