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- W2144709103 abstract "A simple and sensitive fluorescence immunoassay for the detection of aflatoxin B1 (AFB1, as a model compound) in food was developed using AFB1–bovine serum albumin conjugate (AFB1–BSA)-functionalized magnetic beads as immunosensing probes. The recognition elements were prepared by doping of rhodamine B (RB) fluorophore into silica nanoparticles followed by immobilization of monoclonal anti-AFB1 antibodies on the silica shell. Based on a competitive-type immunoassay format, the assay was performed both in low-binding polypropylene 96-well microtiter plates (MTPs) and in an automated sequential injection (SI) format. Similar detection limit (LOD) of 0.2 ng mL−1vs. 0.1 ng mL−1 but narrower dynamic working linear range of 0.5–7 ng mL−1vs. 0.5–30 ng mL−1 was obtained toward AFB1 standards with the flow setup compared to the MTP format. Intra-batch assay precision was substantially improved (≤5.3% vs. ≤8.7%) by resorting to the SI manifold. The proposed method features unbiased identification of negative (blank) and positive samples. No significant differences at the 95% confidence level were encountered in the analysis of naturally contaminated peanut samples between the proposed immunoassay and liquid chromatography for determination of AFB1." @default.
- W2144709103 created "2016-06-24" @default.
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- W2144709103 date "2010-01-01" @default.
- W2144709103 modified "2023-10-02" @default.
- W2144709103 title "Magnetic bead-based fluorescence immunoassay for aflatoxin B1 in food using biofunctionalized rhodamine B-doped silica nanoparticles" @default.
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- W2144709103 doi "https://doi.org/10.1039/c0an00221f" @default.
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