Matches in SemOpenAlex for { <https://semopenalex.org/work/W2144844508> ?p ?o ?g. }
- W2144844508 abstract "Abstract Background Entamoeba histolytica is an intestinal protozoan parasite that causes amoebiasis in humans, affecting up to 50 million people worldwide each year and causing 40,000 to 100,000 deaths annually. EhCP112 is a cysteine proteinase of E. histolytica able to disrupt cell monolayers and digest extracellular matrix proteins, it is secreted by trophozoites and it can be active in a wide range of temperature and pH. These characteristics have encouraged the use of EhCP112 in the design and production of possible vaccines against amoebiasis, obtaining promising results. Nevertheless, we have no conclusive information about the role of EhCP112 in the E. histolytica pathogenesis. Methods A set of three specific siRNA sequences were used to silence the Ehcp112 gene via the soaking system. Silencing was evaluated by Western blot using an antibody against the EhCP112 recombinant protein. Finally, we analyzed the protease activity, the phagocytosis rate and the ability to destroy MDCK cells of the EhCP112-silenced trophozoites. Results The highest silencing effect on EhCP112 was detected at 16 h of treatment; time enough to perform the in vitro virulence assays, which showed that EhCP112 silencing produces a significant reduction in cytolysis and phagocytosis of target cells, indicating the participation of this proteinase in these events. Conclusions EhCP112 is involved in the in vitro virulence of E. histolytica ." @default.
- W2144844508 created "2016-06-24" @default.
- W2144844508 creator A5000661068 @default.
- W2144844508 creator A5042893828 @default.
- W2144844508 creator A5055280434 @default.
- W2144844508 creator A5066659525 @default.
- W2144844508 creator A5082460153 @default.
- W2144844508 creator A5091809030 @default.
- W2144844508 date "2013-08-28" @default.
- W2144844508 modified "2023-09-27" @default.
- W2144844508 title "Effect of the silencing of the Ehcp112 gene on the in vitro virulence of Entamoeba histolytica" @default.
- W2144844508 cites W1538167698 @default.
- W2144844508 cites W1543548512 @default.
- W2144844508 cites W1972689042 @default.
- W2144844508 cites W1981660964 @default.
- W2144844508 cites W1982732754 @default.
- W2144844508 cites W1982851902 @default.
- W2144844508 cites W1987546203 @default.
- W2144844508 cites W1992363176 @default.
- W2144844508 cites W2000661169 @default.
- W2144844508 cites W2009972285 @default.
- W2144844508 cites W2017746602 @default.
- W2144844508 cites W2032630270 @default.
- W2144844508 cites W2040105120 @default.
- W2144844508 cites W2048248702 @default.
- W2144844508 cites W2059964311 @default.
- W2144844508 cites W2062823235 @default.
- W2144844508 cites W2067875953 @default.
- W2144844508 cites W2074788196 @default.
- W2144844508 cites W2077752559 @default.
- W2144844508 cites W2080751376 @default.
- W2144844508 cites W2085457024 @default.
- W2144844508 cites W2087677809 @default.
- W2144844508 cites W2089691466 @default.
- W2144844508 cites W2093060745 @default.
- W2144844508 cites W2094687168 @default.
- W2144844508 cites W2097046100 @default.
- W2144844508 cites W2097313933 @default.
- W2144844508 cites W2099646811 @default.
- W2144844508 cites W2114969015 @default.
- W2144844508 cites W2118421293 @default.
- W2144844508 cites W2143302258 @default.
- W2144844508 cites W2149210551 @default.
- W2144844508 cites W2162351483 @default.
- W2144844508 cites W2162787169 @default.
- W2144844508 cites W2166058841 @default.
- W2144844508 cites W2166668583 @default.
- W2144844508 cites W2167879127 @default.
- W2144844508 cites W2171712234 @default.
- W2144844508 cites W2176557328 @default.
- W2144844508 cites W2182364176 @default.
- W2144844508 cites W4211257403 @default.
- W2144844508 cites W58481436 @default.
- W2144844508 doi "https://doi.org/10.1186/1756-3305-6-248" @default.
- W2144844508 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3765809" @default.
- W2144844508 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23981435" @default.
- W2144844508 hasPublicationYear "2013" @default.
- W2144844508 type Work @default.
- W2144844508 sameAs 2144844508 @default.
- W2144844508 citedByCount "16" @default.
- W2144844508 countsByYear W21448445082014 @default.
- W2144844508 countsByYear W21448445082015 @default.
- W2144844508 countsByYear W21448445082016 @default.
- W2144844508 countsByYear W21448445082017 @default.
- W2144844508 countsByYear W21448445082018 @default.
- W2144844508 countsByYear W21448445082019 @default.
- W2144844508 countsByYear W21448445082021 @default.
- W2144844508 countsByYear W21448445082023 @default.
- W2144844508 crossrefType "journal-article" @default.
- W2144844508 hasAuthorship W2144844508A5000661068 @default.
- W2144844508 hasAuthorship W2144844508A5042893828 @default.
- W2144844508 hasAuthorship W2144844508A5055280434 @default.
- W2144844508 hasAuthorship W2144844508A5066659525 @default.
- W2144844508 hasAuthorship W2144844508A5082460153 @default.
- W2144844508 hasAuthorship W2144844508A5091809030 @default.
- W2144844508 hasBestOaLocation W21448445081 @default.
- W2144844508 hasConcept C104317684 @default.
- W2144844508 hasConcept C119056186 @default.
- W2144844508 hasConcept C159047783 @default.
- W2144844508 hasConcept C160448771 @default.
- W2144844508 hasConcept C181199279 @default.
- W2144844508 hasConcept C182220744 @default.
- W2144844508 hasConcept C202751555 @default.
- W2144844508 hasConcept C2776867272 @default.
- W2144844508 hasConcept C2777283782 @default.
- W2144844508 hasConcept C2778455923 @default.
- W2144844508 hasConcept C54355233 @default.
- W2144844508 hasConcept C55493867 @default.
- W2144844508 hasConcept C60987743 @default.
- W2144844508 hasConcept C86803240 @default.
- W2144844508 hasConcept C89423630 @default.
- W2144844508 hasConceptScore W2144844508C104317684 @default.
- W2144844508 hasConceptScore W2144844508C119056186 @default.
- W2144844508 hasConceptScore W2144844508C159047783 @default.
- W2144844508 hasConceptScore W2144844508C160448771 @default.
- W2144844508 hasConceptScore W2144844508C181199279 @default.
- W2144844508 hasConceptScore W2144844508C182220744 @default.
- W2144844508 hasConceptScore W2144844508C202751555 @default.
- W2144844508 hasConceptScore W2144844508C2776867272 @default.
- W2144844508 hasConceptScore W2144844508C2777283782 @default.