Matches in SemOpenAlex for { <https://semopenalex.org/work/W2144859145> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2144859145 endingPage "1509" @default.
- W2144859145 startingPage "1503" @default.
- W2144859145 abstract "The physicochemical characteristics of the lactococcin A immunity protein, as deduced from its gene sequence, were used to devise a procedure for its purification. The protein was purified from cell extracts by cation-exchange and reverse-phase chromatography. As judged from the amino acid composition and amino acid sequencing, the immunity protein is not post-translationally processed by cleavage at its N- or C-terminus. Consequently, the absorption coefficient at 280 nm, the isoelectric point, and the molecular mass of the immunity protein may be calculated to be, respectively, 8.2 x 10(3) M-1 cm-1, 10.2 and 11,163 Da from the amino acid sequence predicted from the nucleotide sequence. The immunity protein is a major cell protein component--one cell may contain (to an order of magnitude) 10(5) molecules--and it is in part associated with the cell membrane, as judged by immunoblot analysis of membrane vesicle-associated proteins. Exposing lactococcin-A-sensitive cells to an excess of the immunity protein did not affect the lactococcin-A-induced killing of the cells, indicating that the immunity protein does not protect cells by simply binding to lactococcin A, nor to externally exposed domains on the cell surface. Exposing immune-positive cells to antiserum against the immune protein did not sensitize the cells to lactococcin A, suggesting that the immunity protein in fact does not act extracellularly." @default.
- W2144859145 created "2016-06-24" @default.
- W2144859145 creator A5010563845 @default.
- W2144859145 creator A5016888452 @default.
- W2144859145 creator A5038558871 @default.
- W2144859145 creator A5043674580 @default.
- W2144859145 creator A5070544965 @default.
- W2144859145 date "1993-07-01" @default.
- W2144859145 modified "2023-09-25" @default.
- W2144859145 title "Association of the lactococcin A immunity factor with the cell membrane: purification and characterization of the immunity factor" @default.
- W2144859145 cites W1485776927 @default.
- W2144859145 cites W1509140125 @default.
- W2144859145 cites W1527928094 @default.
- W2144859145 cites W1548925052 @default.
- W2144859145 cites W1578404661 @default.
- W2144859145 cites W1583265703 @default.
- W2144859145 cites W1741443434 @default.
- W2144859145 cites W1821942429 @default.
- W2144859145 cites W1836083508 @default.
- W2144859145 cites W1880872626 @default.
- W2144859145 cites W1930119484 @default.
- W2144859145 cites W1999291944 @default.
- W2144859145 cites W2069686957 @default.
- W2144859145 cites W2100329115 @default.
- W2144859145 cites W2100837269 @default.
- W2144859145 cites W2103398607 @default.
- W2144859145 cites W2121552999 @default.
- W2144859145 cites W2128635872 @default.
- W2144859145 cites W2159401663 @default.
- W2144859145 cites W2170917404 @default.
- W2144859145 cites W2415991929 @default.
- W2144859145 doi "https://doi.org/10.1099/00221287-139-7-1503" @default.
- W2144859145 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8371113" @default.
- W2144859145 hasPublicationYear "1993" @default.
- W2144859145 type Work @default.
- W2144859145 sameAs 2144859145 @default.
- W2144859145 citedByCount "66" @default.
- W2144859145 countsByYear W21448591452012 @default.
- W2144859145 countsByYear W21448591452014 @default.
- W2144859145 countsByYear W21448591452015 @default.
- W2144859145 countsByYear W21448591452016 @default.
- W2144859145 countsByYear W21448591452017 @default.
- W2144859145 countsByYear W21448591452018 @default.
- W2144859145 countsByYear W21448591452019 @default.
- W2144859145 countsByYear W21448591452022 @default.
- W2144859145 crossrefType "journal-article" @default.
- W2144859145 hasAuthorship W2144859145A5010563845 @default.
- W2144859145 hasAuthorship W2144859145A5016888452 @default.
- W2144859145 hasAuthorship W2144859145A5038558871 @default.
- W2144859145 hasAuthorship W2144859145A5043674580 @default.
- W2144859145 hasAuthorship W2144859145A5070544965 @default.
- W2144859145 hasBestOaLocation W21448591451 @default.
- W2144859145 hasConcept C104317684 @default.
- W2144859145 hasConcept C153911025 @default.
- W2144859145 hasConcept C167625842 @default.
- W2144859145 hasConcept C181199279 @default.
- W2144859145 hasConcept C2779341262 @default.
- W2144859145 hasConcept C3017875465 @default.
- W2144859145 hasConcept C515207424 @default.
- W2144859145 hasConcept C54355233 @default.
- W2144859145 hasConcept C55493867 @default.
- W2144859145 hasConcept C86803240 @default.
- W2144859145 hasConcept C8891405 @default.
- W2144859145 hasConcept C96294017 @default.
- W2144859145 hasConceptScore W2144859145C104317684 @default.
- W2144859145 hasConceptScore W2144859145C153911025 @default.
- W2144859145 hasConceptScore W2144859145C167625842 @default.
- W2144859145 hasConceptScore W2144859145C181199279 @default.
- W2144859145 hasConceptScore W2144859145C2779341262 @default.
- W2144859145 hasConceptScore W2144859145C3017875465 @default.
- W2144859145 hasConceptScore W2144859145C515207424 @default.
- W2144859145 hasConceptScore W2144859145C54355233 @default.
- W2144859145 hasConceptScore W2144859145C55493867 @default.
- W2144859145 hasConceptScore W2144859145C86803240 @default.
- W2144859145 hasConceptScore W2144859145C8891405 @default.
- W2144859145 hasConceptScore W2144859145C96294017 @default.
- W2144859145 hasIssue "7" @default.
- W2144859145 hasLocation W21448591451 @default.
- W2144859145 hasLocation W21448591452 @default.
- W2144859145 hasOpenAccess W2144859145 @default.
- W2144859145 hasPrimaryLocation W21448591451 @default.
- W2144859145 hasRelatedWork W124612284 @default.
- W2144859145 hasRelatedWork W2073375101 @default.
- W2144859145 hasRelatedWork W2352863532 @default.
- W2144859145 hasRelatedWork W2355333459 @default.
- W2144859145 hasRelatedWork W2363549057 @default.
- W2144859145 hasRelatedWork W2371244410 @default.
- W2144859145 hasRelatedWork W2478580149 @default.
- W2144859145 hasRelatedWork W4200545532 @default.
- W2144859145 hasRelatedWork W4292373192 @default.
- W2144859145 hasRelatedWork W4381570565 @default.
- W2144859145 hasVolume "139" @default.
- W2144859145 isParatext "false" @default.
- W2144859145 isRetracted "false" @default.
- W2144859145 magId "2144859145" @default.
- W2144859145 workType "article" @default.