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• W2144870971 abstract "ABSTRACT Objective: We tested the hypothesis that differential stimulation of nitric oxide (NO) production can be induced in pre‐ and postcapillary segments of the microcirculation in the hamster cheek pouch. Materials and Methods: We applied acetylcholine (ACh) or platelet‐activating factor (PAF) topically and measured perivascular NO concentration ([NO]) with NO‐sensitive microelectrodes in arterioles and venules of the hamster cheek pouch. We also measured NO in cultured coronary endothelial cells (CVEC) after ACh or PAF. Results: ACh increased periarteriolar [NO] significantly in a dose‐dependent manner. ACh at 1 μM increased [NO] from 438.1±43.4 nM at baseline to 647.9±66.3 nM, while 10 μM of ACh increased [NO] from baseline to 1,035.0±59.2 nM ( P <0.05). Neither 1 nor 10 μM of ACh changed perivenular [NO] in the hamster cheek pouch. PAF, at 100 nM, increased perivenular [NO] from 326.6±50.8 to 622.8±41.5 nM. Importantly, 100 nM of PAF did not increase periarteriolar [NO]. PAF increased [NO] from 3.6±2.1 to 455.5±19.9 in CVEC, while ACh had no effect. Conclusions: We conclude that NO production can be stimulated in a differential manner in pre‐ and postcapillary segments in the hamster cheek pouch. ACh selectively stimulates the production of NO only in arterioles, while PAF stimulates the production of NO only in venules." @default.
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• W2144870971 date "2009-05-01" @default.
• W2144870971 modified "2023-10-15" @default.
• W2144870971 title "Independent Regulation of Periarteriolar and Perivenular Nitric Oxide Mechanisms in the<i>In Vivo</i>Hamster Cheek Pouch Microvasculature" @default.
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• W2144870971 doi "https://doi.org/10.1080/10739680902734876" @default.
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