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- W2144935519 abstract "DNA β is a circular single-stranded satellite DNA associated with certain monopartite begomoviruses (family Geminiviridae) which causes economically important diseases such as cotton leaf curl disease. DNA β contains a single gene, βC1, which encodes a pathogenicity protein responsible for symptom production. Transient expression studies in Nicotiana tabacum using the β-glucuronidase reporter gene driven by a βC1 promoter-deletion series of the DNA β associated with cotton leaf curl Multan virus identified a 68 nt region (between −139 and −207) which is important for βC1 transcription. This 68 nt region contains a G-box (CACGTG) located 143 nt upstream of the βC1 start codon. Mutation of the G-box resulted in a significant reduction in βC1 promoter activity and DNA β replication efficiency. In addition, the G-box motif was found to bind specifically to a protein(s) in nuclear extracts prepared from tobacco leaf tissues. Our results indicate that interaction of the G-box motif with host nuclear factors is important for efficient gene expression and replication of DNA β." @default.
- W2144935519 created "2016-06-24" @default.
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- W2144935519 date "2009-01-01" @default.
- W2144935519 modified "2023-10-06" @default.
- W2144935519 title "Identification of sequence elements regulating promoter activity and replication of a monopartite begomovirus-associated DNA satellite" @default.
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- W2144935519 doi "https://doi.org/10.1099/vir.0.002980-0" @default.
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