Matches in SemOpenAlex for { <https://semopenalex.org/work/W2145089114> ?p ?o ?g. }
- W2145089114 abstract "Abstract Background Knockout of either toll-like receptor 4 (TLR4) or 2 (TLR2) had been reported to delay the Wallerian degeneration after peripheral nerve injury by deterring the recruitment of the macrophages and clearance of myelin debris. However, the impact on the remyelination process is poorly understood. In this study, the effect of TLR2 and TLR4 knockout on the nerve regeneration and on the remyelination process was studied in a mouse model of sciatic nerve crush injury. Results A standard sciatic nerve crush injury by a No. 5 Jeweler forcep for consistent 30 seconds was performed in Tlr4 −/− (B6.B10ScN- Tlr4 lps-del /JthJ), Tlr2 −/− (B6.129-Tlr2 tm1Kir /J) and C57BL/6 mice. One centimeter of nerve segment distal to the crushed site was harvested for western blot analysis of the myelin structure protein myelin protein zero (Mpz) and the remyelination transcription factors Oct6 and Sox10 at day 0, 3, 7, 10, 14, 17, 21, 28. Nerve segment 5-mm distal to injured site from additional groups of mice at day 10 after crush injury were subjected to semi-thin section and toluidine blue stain for a quantitative histomorphometric analysis. With less remyelinated nerves and more nerve debris, the histomorphometric analysis revealed a worse nerve regeneration following the sciatic nerve crush injury in both Tlr4 −/− and Tlr2 −/− mice than the C57BL/6 mice. Although there was a delayed expression of Sox10 but not Oct6 during remyelination, with an average 4-day delay in the demyelination process, the subsequent complete formation of Mpz during remyelination was also delayed for 4 days, implying that the impaired nerve regeneration was mainly attributed to the delayed demyelination process. Conclusions Both TLR4 and TLR2 are crucial for nerve regeneration after nerve crush injury mainly by delaying the demyelination but not the remyelination process." @default.
- W2145089114 created "2016-06-24" @default.
- W2145089114 creator A5015141498 @default.
- W2145089114 creator A5058833388 @default.
- W2145089114 creator A5070086522 @default.
- W2145089114 creator A5076031794 @default.
- W2145089114 creator A5082539859 @default.
- W2145089114 creator A5089427275 @default.
- W2145089114 creator A5089671704 @default.
- W2145089114 creator A5090916333 @default.
- W2145089114 date "2013-08-28" @default.
- W2145089114 modified "2023-10-16" @default.
- W2145089114 title "Knockout of TLR4 and TLR2 impair the nerve regeneration by delayed demyelination but not remyelination" @default.
- W2145089114 cites W105373620 @default.
- W2145089114 cites W1850163235 @default.
- W2145089114 cites W1964571549 @default.
- W2145089114 cites W1965932992 @default.
- W2145089114 cites W1974578353 @default.
- W2145089114 cites W1980469138 @default.
- W2145089114 cites W1981632014 @default.
- W2145089114 cites W1997795701 @default.
- W2145089114 cites W2004394976 @default.
- W2145089114 cites W2004773897 @default.
- W2145089114 cites W2008431619 @default.
- W2145089114 cites W2019919875 @default.
- W2145089114 cites W2034670933 @default.
- W2145089114 cites W2035233037 @default.
- W2145089114 cites W2036747221 @default.
- W2145089114 cites W2051633940 @default.
- W2145089114 cites W2056594032 @default.
- W2145089114 cites W2057649701 @default.
- W2145089114 cites W2064209588 @default.
- W2145089114 cites W2066181529 @default.
- W2145089114 cites W2068059177 @default.
- W2145089114 cites W2068639096 @default.
- W2145089114 cites W2069871156 @default.
- W2145089114 cites W2076347916 @default.
- W2145089114 cites W2080027570 @default.
- W2145089114 cites W2083610490 @default.
- W2145089114 cites W2086333602 @default.
- W2145089114 cites W2091968762 @default.
- W2145089114 cites W2093229003 @default.
- W2145089114 cites W2096027130 @default.
- W2145089114 cites W2101363646 @default.
- W2145089114 cites W2107826905 @default.
- W2145089114 cites W2109491705 @default.
- W2145089114 cites W2116547262 @default.
- W2145089114 cites W2126974532 @default.
- W2145089114 cites W2136049931 @default.
- W2145089114 cites W2144161917 @default.
- W2145089114 cites W2145736817 @default.
- W2145089114 cites W2152524281 @default.
- W2145089114 doi "https://doi.org/10.1186/1423-0127-20-62" @default.
- W2145089114 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3765918" @default.
- W2145089114 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23984978" @default.
- W2145089114 hasPublicationYear "2013" @default.
- W2145089114 type Work @default.
- W2145089114 sameAs 2145089114 @default.
- W2145089114 citedByCount "20" @default.
- W2145089114 countsByYear W21450891142014 @default.
- W2145089114 countsByYear W21450891142015 @default.
- W2145089114 countsByYear W21450891142016 @default.
- W2145089114 countsByYear W21450891142017 @default.
- W2145089114 countsByYear W21450891142018 @default.
- W2145089114 countsByYear W21450891142019 @default.
- W2145089114 countsByYear W21450891142021 @default.
- W2145089114 countsByYear W21450891142022 @default.
- W2145089114 countsByYear W21450891142023 @default.
- W2145089114 crossrefType "journal-article" @default.
- W2145089114 hasAuthorship W2145089114A5015141498 @default.
- W2145089114 hasAuthorship W2145089114A5058833388 @default.
- W2145089114 hasAuthorship W2145089114A5070086522 @default.
- W2145089114 hasAuthorship W2145089114A5076031794 @default.
- W2145089114 hasAuthorship W2145089114A5082539859 @default.
- W2145089114 hasAuthorship W2145089114A5089427275 @default.
- W2145089114 hasAuthorship W2145089114A5089671704 @default.
- W2145089114 hasAuthorship W2145089114A5090916333 @default.
- W2145089114 hasBestOaLocation W21450891141 @default.
- W2145089114 hasConcept C105702510 @default.
- W2145089114 hasConcept C134018914 @default.
- W2145089114 hasConcept C141071460 @default.
- W2145089114 hasConcept C142724271 @default.
- W2145089114 hasConcept C171056886 @default.
- W2145089114 hasConcept C2775991916 @default.
- W2145089114 hasConcept C2776880756 @default.
- W2145089114 hasConcept C2777107032 @default.
- W2145089114 hasConcept C2777619251 @default.
- W2145089114 hasConcept C2778609137 @default.
- W2145089114 hasConcept C2780862704 @default.
- W2145089114 hasConcept C2781149210 @default.
- W2145089114 hasConcept C42219234 @default.
- W2145089114 hasConcept C529278444 @default.
- W2145089114 hasConcept C71924100 @default.
- W2145089114 hasConcept C86803240 @default.
- W2145089114 hasConcept C90224389 @default.
- W2145089114 hasConcept C95444343 @default.
- W2145089114 hasConceptScore W2145089114C105702510 @default.
- W2145089114 hasConceptScore W2145089114C134018914 @default.
- W2145089114 hasConceptScore W2145089114C141071460 @default.
- W2145089114 hasConceptScore W2145089114C142724271 @default.