FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2145148510> ?p ?o ?g. }

• W2145148510 endingPage "629" @default.
• W2145148510 startingPage "614" @default.
• W2145148510 abstract "Previous studies have shown that early mouse embryos with both FGF-4 alleles inactivated are developmentally arrested shortly after implantation. To understand the roles of FGF-4 during early development, we prepared genetically engineered embryonic stem (ES) cells, which are unable to produce FGF-4. Specifically, we describe the isolation and characterization of ES cells with both FGF-4 alleles inactivated. The FGF-4−/−ES cells do not require FGF-4 to proliferatein vitro,and addition of FGF-4 to the medium has little or no effect on their growth. Thus, FGF-4 does not appear to act as an autocrine growth factor for cultured ES cells. We also demonstrate that FGF-4−/−ES cells, like their unmodified counterparts, are capable of forming highly complex tumors in syngeneic mice composed of a wide range of differentiated cells types, including neural tissue, glandular epithelium, and muscle. In addition, we demonstrate that the FGF-4−/−ES cells can differentiatein vitroafter exposure to retinoic acid; however, the growth and/or survival of the differentiated cells is severely compromised. Importantly, addition of FGF-4 to the culture medium dramatically increases the number of differentiated cells derived from the FGF-4−/−ES cells, in particular cells with many of the properties of parietal extraembryonic endoderm. Finally, we demonstrate that there are differences in the RNA profiles expressed by the differentiated progeny formedin vitrofrom FGF-4−/−ES cells and FGF-4+/+ES cells when they are cultured with FGF-4. Taken together, the studies described in this report indicate that certain lineages formedin vitroare affected by the inactivation of the FGF-4 gene, in particular specific cells that form during the initial stage of ES cell differentiation. Thus, ES cells with both FGF-4 alleles inactivated should shed light on the important roles of FGF-4 during the early stages of mammalian development and help determine why FGF-4−/−embryos die shortly after implantation." @default.
• W2145148510 created "2016-06-24" @default.
• W2145148510 creator A5004367777 @default.
• W2145148510 creator A5019734790 @default.
• W2145148510 creator A5026584694 @default.
• W2145148510 creator A5055590238 @default.
• W2145148510 creator A5057292551 @default.
• W2145148510 creator A5062582917 @default.
• W2145148510 creator A5065080973 @default.
• W2145148510 creator A5066738691 @default.
• W2145148510 creator A5088746193 @default.
• W2145148510 date "1997-12-01" @default.
• W2145148510 modified "2023-09-30" @default.
• W2145148510 title "Inactivation of the FGF-4 Gene in Embryonic Stem Cells Alters the Growth and/or the Survival of Their Early Differentiated Progeny" @default.
• W2145148510 cites W1521871611 @default.
• W2145148510 cites W1925019465 @default.
• W2145148510 cites W1951882906 @default.
• W2145148510 cites W1966697925 @default.
• W2145148510 cites W1967485093 @default.
• W2145148510 cites W1971943177 @default.
• W2145148510 cites W1978858094 @default.
• W2145148510 cites W1979943314 @default.
• W2145148510 cites W1983237714 @default.
• W2145148510 cites W1985360296 @default.
• W2145148510 cites W1988500526 @default.
• W2145148510 cites W1989111076 @default.
• W2145148510 cites W1993834457 @default.
• W2145148510 cites W1998118848 @default.
• W2145148510 cites W2011282428 @default.
• W2145148510 cites W2016253997 @default.
• W2145148510 cites W2016274629 @default.
• W2145148510 cites W2023817261 @default.
• W2145148510 cites W2029965703 @default.
• W2145148510 cites W2036851890 @default.
• W2145148510 cites W2039358114 @default.
• W2145148510 cites W2040648928 @default.
• W2145148510 cites W2047727235 @default.
• W2145148510 cites W2051780160 @default.
• W2145148510 cites W2056016311 @default.
• W2145148510 cites W2058528119 @default.
• W2145148510 cites W2060029591 @default.
• W2145148510 cites W2068281991 @default.
• W2145148510 cites W2069022895 @default.
• W2145148510 cites W2077045259 @default.
• W2145148510 cites W2080432157 @default.
• W2145148510 cites W2081959372 @default.
• W2145148510 cites W2084156830 @default.
• W2145148510 cites W2094297257 @default.
• W2145148510 cites W2118094114 @default.
• W2145148510 cites W2131986778 @default.
• W2145148510 cites W2138354687 @default.
• W2145148510 cites W2142534050 @default.
• W2145148510 cites W2143238964 @default.
• W2145148510 cites W2158078570 @default.
• W2145148510 cites W2168117739 @default.
• W2145148510 cites W2181453860 @default.
• W2145148510 cites W2184559181 @default.
• W2145148510 cites W2189550019 @default.
• W2145148510 cites W4241531133 @default.
• W2145148510 cites W4246131267 @default.
• W2145148510 doi "https://doi.org/10.1006/dbio.1997.8777" @default.
• W2145148510 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9441693" @default.
• W2145148510 hasPublicationYear "1997" @default.
• W2145148510 type Work @default.
• W2145148510 sameAs 2145148510 @default.
• W2145148510 citedByCount "119" @default.
• W2145148510 countsByYear W21451485102012 @default.
• W2145148510 countsByYear W21451485102013 @default.
• W2145148510 countsByYear W21451485102014 @default.
• W2145148510 countsByYear W21451485102015 @default.
• W2145148510 countsByYear W21451485102016 @default.
• W2145148510 countsByYear W21451485102017 @default.
• W2145148510 countsByYear W21451485102019 @default.
• W2145148510 countsByYear W21451485102020 @default.
• W2145148510 countsByYear W21451485102023 @default.
• W2145148510 crossrefType "journal-article" @default.
• W2145148510 hasAuthorship W2145148510A5004367777 @default.
• W2145148510 hasAuthorship W2145148510A5019734790 @default.
• W2145148510 hasAuthorship W2145148510A5026584694 @default.
• W2145148510 hasAuthorship W2145148510A5055590238 @default.
• W2145148510 hasAuthorship W2145148510A5057292551 @default.
• W2145148510 hasAuthorship W2145148510A5062582917 @default.
• W2145148510 hasAuthorship W2145148510A5065080973 @default.
• W2145148510 hasAuthorship W2145148510A5066738691 @default.
• W2145148510 hasAuthorship W2145148510A5088746193 @default.
• W2145148510 hasBestOaLocation W21451485101 @default.
• W2145148510 hasConcept C104317684 @default.
• W2145148510 hasConcept C128240485 @default.
• W2145148510 hasConcept C145103041 @default.
• W2145148510 hasConcept C148738053 @default.
• W2145148510 hasConcept C153911025 @default.
• W2145148510 hasConcept C162746124 @default.
• W2145148510 hasConcept C170493617 @default.
• W2145148510 hasConcept C202751555 @default.
• W2145148510 hasConcept C20875687 @default.
• W2145148510 hasConcept C28328180 @default.
• W2145148510 hasConcept C54355233 @default.
• W2145148510 hasConcept C74373430 @default.

• next