FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2145148604> ?p ?o ?g. }

• W2145148604 endingPage "774" @default.
• W2145148604 startingPage "763" @default.
• W2145148604 abstract "An alkaline serine protease producing strain Alternaria solani was optimized for its enzyme production under submerged conditions. The maximum production of protease by A. solani was achieved by using sodium nitrate at the optimum concentration of 0.2% w/v. A. solani produced higher quantities (3.75 [unit/mg of protein]) of an inducible extracellular proteases on day 9 after incubation in czapek's dox broth medium amended with 1% casein as an inducer at pH 8.5, temperature 27 °C and 3% sucrose as carbon source. Extracellular proteases were precipitated by ammonium sulphate saturation (80%) method and purified on Sephadex G‐100 column chromatography. The molecular mass of SDS–PAGE and Sephadex G‐100 Column Gel permeation chromatography purified protease was estimated to 42 kDa. In addition, trypsin digestion of 42 kDa protein band was carried out and analyzed by MALDI–TOF for the identification of protease. The sequence IKELATNGVVTNVK (378–391) segment of the alkaline serine protease was found by using MS/MS spectrum at 1485 m/z from the purified fraction. It showed optimal activity at 50 °C and pH 9–10 and broad pH stability between pH 6–12. The protease activity was inhibited by phenyl methyl sulfonyl fluoride (PMSF), all the results indicated that the presence of a serine residue in the active site and is thus most likely a member of the serine protease family. This may function as a virulence protein during pathogenesis by A. solani . The results suggested that the presence of appreciable extracellular proteolytic activity in filamentous fungi may serve as a marker of their phytopathogenicity." @default.
• W2145148604 created "2016-06-24" @default.
• W2145148604 creator A5012219359 @default.
• W2145148604 creator A5062104014 @default.
• W2145148604 date "2013-05-28" @default.
• W2145148604 modified "2023-10-18" @default.
• W2145148604 title "Production, partial purification and characterization of protease from a phytopathogenic fungi<i>Alternaria solani</i>(Ell. and Mart.) Sorauer" @default.
• W2145148604 cites W126109032 @default.
• W2145148604 cites W136895204 @default.
• W2145148604 cites W1515052776 @default.
• W2145148604 cites W1898899845 @default.
• W2145148604 cites W1971054163 @default.
• W2145148604 cites W1976375835 @default.
• W2145148604 cites W1982217741 @default.
• W2145148604 cites W1988254871 @default.
• W2145148604 cites W1989359326 @default.
• W2145148604 cites W1990726117 @default.
• W2145148604 cites W1995155209 @default.
• W2145148604 cites W1996148094 @default.
• W2145148604 cites W1996693179 @default.
• W2145148604 cites W2008419295 @default.
• W2145148604 cites W2023586798 @default.
• W2145148604 cites W2032350170 @default.
• W2145148604 cites W2046183956 @default.
• W2145148604 cites W2048130717 @default.
• W2145148604 cites W2055237511 @default.
• W2145148604 cites W2071187955 @default.
• W2145148604 cites W2078750873 @default.
• W2145148604 cites W2079831464 @default.
• W2145148604 cites W2087888992 @default.
• W2145148604 cites W2089172046 @default.
• W2145148604 cites W2094321878 @default.
• W2145148604 cites W2100837269 @default.
• W2145148604 cites W2102628698 @default.
• W2145148604 cites W2103196178 @default.
• W2145148604 cites W2118599485 @default.
• W2145148604 cites W2127773227 @default.
• W2145148604 cites W2132528591 @default.
• W2145148604 cites W2138357457 @default.
• W2145148604 cites W2146126534 @default.
• W2145148604 cites W2614999422 @default.
• W2145148604 cites W3878945 @default.
• W2145148604 cites W4238052567 @default.
• W2145148604 cites W4252914174 @default.
• W2145148604 cites W4293247451 @default.
• W2145148604 cites W4366075600 @default.
• W2145148604 doi "https://doi.org/10.1002/jobm.201200584" @default.
• W2145148604 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23712744" @default.
• W2145148604 hasPublicationYear "2013" @default.
• W2145148604 type Work @default.
• W2145148604 sameAs 2145148604 @default.
• W2145148604 citedByCount "20" @default.
• W2145148604 countsByYear W21451486042013 @default.
• W2145148604 countsByYear W21451486042015 @default.
• W2145148604 countsByYear W21451486042016 @default.
• W2145148604 countsByYear W21451486042018 @default.
• W2145148604 countsByYear W21451486042019 @default.
• W2145148604 countsByYear W21451486042020 @default.
• W2145148604 countsByYear W21451486042021 @default.
• W2145148604 countsByYear W21451486042022 @default.
• W2145148604 countsByYear W21451486042023 @default.
• W2145148604 crossrefType "journal-article" @default.
• W2145148604 hasAuthorship W2145148604A5012219359 @default.
• W2145148604 hasAuthorship W2145148604A5062104014 @default.
• W2145148604 hasConcept C163994747 @default.
• W2145148604 hasConcept C181199279 @default.
• W2145148604 hasConcept C182220744 @default.
• W2145148604 hasConcept C185592680 @default.
• W2145148604 hasConcept C2776142715 @default.
• W2145148604 hasConcept C2776714187 @default.
• W2145148604 hasConcept C2777807008 @default.
• W2145148604 hasConcept C2780865716 @default.
• W2145148604 hasConcept C43617362 @default.
• W2145148604 hasConcept C55493867 @default.
• W2145148604 hasConceptScore W2145148604C163994747 @default.
• W2145148604 hasConceptScore W2145148604C181199279 @default.
• W2145148604 hasConceptScore W2145148604C182220744 @default.
• W2145148604 hasConceptScore W2145148604C185592680 @default.
• W2145148604 hasConceptScore W2145148604C2776142715 @default.
• W2145148604 hasConceptScore W2145148604C2776714187 @default.
• W2145148604 hasConceptScore W2145148604C2777807008 @default.
• W2145148604 hasConceptScore W2145148604C2780865716 @default.
• W2145148604 hasConceptScore W2145148604C43617362 @default.
• W2145148604 hasConceptScore W2145148604C55493867 @default.
• W2145148604 hasIssue "8" @default.
• W2145148604 hasLocation W21451486041 @default.
• W2145148604 hasLocation W21451486042 @default.
• W2145148604 hasOpenAccess W2145148604 @default.
• W2145148604 hasPrimaryLocation W21451486041 @default.
• W2145148604 hasRelatedWork W1044110943 @default.
• W2145148604 hasRelatedWork W1565994824 @default.
• W2145148604 hasRelatedWork W1976608269 @default.
• W2145148604 hasRelatedWork W1979365401 @default.
• W2145148604 hasRelatedWork W2026431744 @default.
• W2145148604 hasRelatedWork W2061462485 @default.
• W2145148604 hasRelatedWork W2072272548 @default.
• W2145148604 hasRelatedWork W2318580069 @default.
• W2145148604 hasRelatedWork W2912636955 @default.

• next