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- W2145278045 abstract "By incubating total protein extracts of Schistosoma mansoni with 3H-17-beta-estradiol and 20-hydroxyecdysone, steroid binding proteins were detected in both male and female worms. The interaction of nuclear proteins with a restriction fragment of the gender and stage-specific gene F-10 was investigated using the 'band-shift' technique. Male and female nuclear proteins bound in a distinct way to the fragment of this gene containing putative regulatory consensus motifs. Among the nuclear proteins, only those rich in cysteine residues bound to DNA. In vitro incubation of live worms with the oestrogen antagonist Tamoxifen, altered the pattern of the DNA binding proteins, producing in females a profile similar to that obtained with male worm protein extracts. This effect of Tamoxifen could not be correlated to inhibition of protein biosynthesis. These results suggest that the regulation of transcription of the F-10 gene might involve steroid receptors." @default.
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- W2145278045 date "1995-02-01" @default.
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- W2145278045 title "F-10 nuclear binding proteins ofSchistosoma mansoni: structural and functional features" @default.
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- W2145278045 doi "https://doi.org/10.1017/s0031182000063915" @default.
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