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- W2145288036 abstract "Continuous exposure of a BALB/c myeloma line to anti-H-2d antibody in the absence of complement, for 3 hours at 37 °C, results in the loss of sensitivity to lysis by added complement or by added anti-H-2d and complement (antigenic modulation). 125I-labeled anti-H-2d was used to follow the fate of the modulating antibody. Iodinated rabbit anti-mouse Ig was used to detect anti-H-2 Ig remaining on the cell surface at various times. It was found that the amount of anti-H-2 antibody detectable on the cell surface decreases by 1/3 to 1/2, and that this decrease occurs by internalization of the bound antibody (and presumably of the antigen). Maintenance of antigenic modulation over a 24-hour period involves a steady state of continuous binding of free antibody and its internalization. Recovery from antigenic modulation occurs during 3 hours after removal of the anti-H-2 antibody and involves a decrease in the amount of mouse anti-H-2d remaining on the cell surface (as detected by labeled rabbit anti-mouse Ig) and an increase in the amount of surface antigen detected by the ability to bind 125I-labeled anti-H-2d." @default.
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- W2145288036 date "1974-11-01" @default.
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- W2145288036 title "Antibody-induced changes in expression of the H-2 antigen" @default.
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- W2145288036 doi "https://doi.org/10.1002/eji.1830041106" @default.
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