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• W2145308519 abstract "We have developed a novel method of DNA extraction combined with a high-throughput method of gene detection allowing thousands of potentially transgenic chicks to be screened quickly and reliably. By using this method and a replication-deficient retroviral vector based on avian leukosis virus (ALV), we have demonstrated germline transmission of three different transgenes. Several generations of chickens carrying intact transgenes were produced, validating the use of the ALV retroviral vectors for large-scale production of transgenic flocks. Fourth-generation chicks that were nontransgenic, hemizygous, or homozygous for the transgene were identified with the combined genetic screening methods." @default.
• W2145308519 created "2016-06-24" @default.
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• W2145308519 date "2002-02-01" @default.
• W2145308519 modified "2023-10-14" @default.
• W2145308519 title "Consistent Production of Transgenic Chickens Using Replication-Deficient Retroviral Vectors and High-Throughput Screening Procedures" @default.
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• W2145308519 doi "https://doi.org/10.1093/ps/81.2.202" @default.
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