FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2145327320> ?p ?o ?g. }

• W2145327320 endingPage "353" @default.
• W2145327320 startingPage "345" @default.
• W2145327320 abstract "Investigations on the mechanisms of IgE regulation in vitro have been conducted thus far in systems that allow the synthesis of total rather than specific IgE. To study the regulatory prerequisites of antigen-specific IgE antibody production, we have established a culture system that allows the generation of bee venom phospholipase A2-specific IgE and IgG4 antibodies. Allergen-specific IgE was induced by simultaneously activating T cells and B cells specifically with allergen and polyclonally with anti-CD2 and soluble CD40 ligand in the presence of IL-4. Additional stimulation of T cells through the CD2 activation pathway by two different anti-CD2 monoclonal antibodies enhanced both the allergen-specific and the total IgE and IgG4 responses. An optimal amount of allergen (0.1 ng/ml) resulted in the induction of both allergen-specific IgE and IgG4 antibodies. Higher antigen doses reduced allergen-specific antibodies and enhanced total isotype production. This differential regulation of allergen-specific and total isotypes reflects different allergen dose-dependent mechanisms in specific and polyclonal activation of T and B cells. Although both isotypes require IL-4 for initial induction, opposite regulatory effects by T cells were observed for IgE and IgG4 antibody expression. In peripheral blood mononuclear cell cultures stimulated with soluble CD40 ligand, IL-4, and phospholipase A2, stimulation of T cells with higher amounts of anti-CD2 enhanced IgG4 in parallel to increased IL-2 and interferon-gamma secretion but inhibited IgE synthesis. These results provide evidence for differential regulation of allergen-specific and total IgE and IgG4 by antigen concentration and demonstrate the pivotal role of T cells controlling the synthesis of the IgE and IgG4 antibody isotypes." @default.
• W2145327320 created "2016-06-24" @default.
• W2145327320 creator A5019919684 @default.
• W2145327320 creator A5024583995 @default.
• W2145327320 creator A5028467411 @default.
• W2145327320 creator A5047566031 @default.
• W2145327320 creator A5055404517 @default.
• W2145327320 creator A5085680989 @default.
• W2145327320 creator A5085722194 @default.
• W2145327320 date "1997-03-01" @default.
• W2145327320 modified "2023-09-23" @default.
• W2145327320 title "Induction and differential regulation of bee venom phospholipase A2–specific human IgE and IgG4 antibodies in vitro requires allergen-specific and nonspecific activation of T and B cells☆, ☆☆, ★, ★★" @default.
• W2145327320 cites W1506157243 @default.
• W2145327320 cites W1531177247 @default.
• W2145327320 cites W1539910874 @default.
• W2145327320 cites W1546231734 @default.
• W2145327320 cites W1557604985 @default.
• W2145327320 cites W1574705898 @default.
• W2145327320 cites W160741039 @default.
• W2145327320 cites W1641557591 @default.
• W2145327320 cites W1654343738 @default.
• W2145327320 cites W173546081 @default.
• W2145327320 cites W1831711338 @default.
• W2145327320 cites W18707814 @default.
• W2145327320 cites W1922117764 @default.
• W2145327320 cites W1943925887 @default.
• W2145327320 cites W1968320073 @default.
• W2145327320 cites W1969912242 @default.
• W2145327320 cites W1971686295 @default.
• W2145327320 cites W1979492370 @default.
• W2145327320 cites W1987968095 @default.
• W2145327320 cites W1989055057 @default.
• W2145327320 cites W1989305940 @default.
• W2145327320 cites W1989692667 @default.
• W2145327320 cites W1994797557 @default.
• W2145327320 cites W2012612191 @default.
• W2145327320 cites W2012852864 @default.
• W2145327320 cites W2016718549 @default.
• W2145327320 cites W2017985531 @default.
• W2145327320 cites W2019454556 @default.
• W2145327320 cites W2020382886 @default.
• W2145327320 cites W2026953619 @default.
• W2145327320 cites W2030698538 @default.
• W2145327320 cites W2032822229 @default.
• W2145327320 cites W2048405383 @default.
• W2145327320 cites W2051918665 @default.
• W2145327320 cites W2054768922 @default.
• W2145327320 cites W2058050336 @default.
• W2145327320 cites W2060382742 @default.
• W2145327320 cites W2062319893 @default.
• W2145327320 cites W2074791128 @default.
• W2145327320 cites W2076074155 @default.
• W2145327320 cites W2085153490 @default.
• W2145327320 cites W2085209020 @default.
• W2145327320 cites W2087170053 @default.
• W2145327320 cites W2088464284 @default.
• W2145327320 cites W2093385116 @default.
• W2145327320 cites W2097225932 @default.
• W2145327320 cites W2103839057 @default.
• W2145327320 cites W2115531618 @default.
• W2145327320 cites W2128782103 @default.
• W2145327320 cites W2132994161 @default.
• W2145327320 cites W2134514926 @default.
• W2145327320 cites W2146722884 @default.
• W2145327320 cites W2160410700 @default.
• W2145327320 doi "https://doi.org/10.1016/s0091-6749(97)70052-6" @default.
• W2145327320 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9058690" @default.
• W2145327320 hasPublicationYear "1997" @default.
• W2145327320 type Work @default.
• W2145327320 sameAs 2145327320 @default.
• W2145327320 citedByCount "60" @default.
• W2145327320 countsByYear W21453273202013 @default.
• W2145327320 countsByYear W21453273202014 @default.
• W2145327320 countsByYear W21453273202015 @default.
• W2145327320 countsByYear W21453273202016 @default.
• W2145327320 countsByYear W21453273202017 @default.
• W2145327320 countsByYear W21453273202021 @default.
• W2145327320 countsByYear W21453273202022 @default.
• W2145327320 crossrefType "journal-article" @default.
• W2145327320 hasAuthorship W2145327320A5019919684 @default.
• W2145327320 hasAuthorship W2145327320A5024583995 @default.
• W2145327320 hasAuthorship W2145327320A5028467411 @default.
• W2145327320 hasAuthorship W2145327320A5047566031 @default.
• W2145327320 hasAuthorship W2145327320A5055404517 @default.
• W2145327320 hasAuthorship W2145327320A5085680989 @default.
• W2145327320 hasAuthorship W2145327320A5085722194 @default.
• W2145327320 hasConcept C141105273 @default.
• W2145327320 hasConcept C159654299 @default.
• W2145327320 hasConcept C185592680 @default.
• W2145327320 hasConcept C203014093 @default.
• W2145327320 hasConcept C207480886 @default.
• W2145327320 hasConcept C2777991845 @default.
• W2145327320 hasConcept C2780510475 @default.
• W2145327320 hasConcept C542903549 @default.
• W2145327320 hasConcept C86803240 @default.
• W2145327320 hasConcept C956191 @default.
• W2145327320 hasConceptScore W2145327320C141105273 @default.
• W2145327320 hasConceptScore W2145327320C159654299 @default.

• next