FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2145483252> ?p ?o ?g. }

• W2145483252 endingPage "101505" @default.
• W2145483252 startingPage "101505" @default.
• W2145483252 abstract "Time-resolved spectrometry of endogenous nicotinamide dinucleotide phosphate [NAD(P)H] fluorescence is a useful method to evaluate metabolic oxidative state in living cells. Ouabain is a well-known pharmaceutical drug used in the treatment of cardiovascular disease, the effects of which on myocardial metabolism were recently demonstrated. Mechanisms implicated in these actions are still poorly understood. We investigate the effect of ouabain on the metabolic oxidative state of living cardiac cells identified by time-resolved fluorescence spectroscopy of mitochondrial NAD(P)H. Spectral unmixing is used to resolve individual NAD(P)H fluorescence components. Ouabain decreased the integral intensity of NAD(P)H fluorescence, leading to a reduced component amplitudes ratio corresponding to a change in metabolic state. We also noted that lactate/pyruvate, affecting the cytosolic NADH gradient, increased the effect of ouabain on the component amplitudes ratio. Cell oxidation levels, evaluated as the percentage of oxidized NAD(P)H, decreased exponentially with rising concentrations of the cardiac glycoside. Ouabain also stimulated the mitochondrial NADH production. Our study sheds a new light on the role that ouabain plays in the regulation of metabolic state, and presents perspective on a noninvasive, pharmaceutical approach for testing the effect of drugs on the mitochondrial metabolism by means of time-resolved fluorescence spectroscopy in living cells." @default.
• W2145483252 created "2016-06-24" @default.
• W2145483252 creator A5048065991 @default.
• W2145483252 creator A5054059185 @default.
• W2145483252 creator A5086356526 @default.
• W2145483252 creator A5088532684 @default.
• W2145483252 date "2012-05-14" @default.
• W2145483252 modified "2023-10-18" @default.
• W2145483252 title "Effect of ouabain on metabolic oxidative state in living cardiomyocytes evaluated by time-resolved spectroscopy of endogenous NAD(P)H fluorescence" @default.
• W2145483252 cites W138505475 @default.
• W2145483252 cites W1479702206 @default.
• W2145483252 cites W1585518597 @default.
• W2145483252 cites W1611437588 @default.
• W2145483252 cites W1899366853 @default.
• W2145483252 cites W1966218746 @default.
• W2145483252 cites W1969197079 @default.
• W2145483252 cites W1969302385 @default.
• W2145483252 cites W1972665998 @default.
• W2145483252 cites W1976270302 @default.
• W2145483252 cites W1993048177 @default.
• W2145483252 cites W1996582791 @default.
• W2145483252 cites W2011173660 @default.
• W2145483252 cites W2030583987 @default.
• W2145483252 cites W2039008255 @default.
• W2145483252 cites W2062737858 @default.
• W2145483252 cites W2063623305 @default.
• W2145483252 cites W2072376785 @default.
• W2145483252 cites W2092901268 @default.
• W2145483252 cites W2111920179 @default.
• W2145483252 cites W2121508606 @default.
• W2145483252 cites W2127179996 @default.
• W2145483252 cites W2135174215 @default.
• W2145483252 cites W2143478068 @default.
• W2145483252 cites W2143617499 @default.
• W2145483252 cites W2144470409 @default.
• W2145483252 cites W2160261406 @default.
• W2145483252 cites W2163171374 @default.
• W2145483252 cites W2167375844 @default.
• W2145483252 cites W2167754090 @default.
• W2145483252 cites W2341692797 @default.
• W2145483252 cites W4237517557 @default.
• W2145483252 cites W4240353687 @default.
• W2145483252 cites W4248703133 @default.
• W2145483252 doi "https://doi.org/10.1117/1.jbo.17.10.101505" @default.
• W2145483252 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23223981" @default.
• W2145483252 hasPublicationYear "2012" @default.
• W2145483252 type Work @default.
• W2145483252 sameAs 2145483252 @default.
• W2145483252 citedByCount "19" @default.
• W2145483252 countsByYear W21454832522012 @default.
• W2145483252 countsByYear W21454832522013 @default.
• W2145483252 countsByYear W21454832522014 @default.
• W2145483252 countsByYear W21454832522015 @default.
• W2145483252 countsByYear W21454832522016 @default.
• W2145483252 countsByYear W21454832522017 @default.
• W2145483252 countsByYear W21454832522018 @default.
• W2145483252 countsByYear W21454832522021 @default.
• W2145483252 countsByYear W21454832522023 @default.
• W2145483252 crossrefType "journal-article" @default.
• W2145483252 hasAuthorship W2145483252A5048065991 @default.
• W2145483252 hasAuthorship W2145483252A5054059185 @default.
• W2145483252 hasAuthorship W2145483252A5086356526 @default.
• W2145483252 hasAuthorship W2145483252A5088532684 @default.
• W2145483252 hasBestOaLocation W21454832521 @default.
• W2145483252 hasConcept C121332964 @default.
• W2145483252 hasConcept C12554922 @default.
• W2145483252 hasConcept C158711907 @default.
• W2145483252 hasConcept C16613235 @default.
• W2145483252 hasConcept C178790620 @default.
• W2145483252 hasConcept C181199279 @default.
• W2145483252 hasConcept C185592680 @default.
• W2145483252 hasConcept C2776901170 @default.
• W2145483252 hasConcept C2779133857 @default.
• W2145483252 hasConcept C28859421 @default.
• W2145483252 hasConcept C537181965 @default.
• W2145483252 hasConcept C55493867 @default.
• W2145483252 hasConcept C57600042 @default.
• W2145483252 hasConcept C62231903 @default.
• W2145483252 hasConcept C62520636 @default.
• W2145483252 hasConcept C75520062 @default.
• W2145483252 hasConcept C86803240 @default.
• W2145483252 hasConcept C91881484 @default.
• W2145483252 hasConcept C98539663 @default.
• W2145483252 hasConceptScore W2145483252C121332964 @default.
• W2145483252 hasConceptScore W2145483252C12554922 @default.
• W2145483252 hasConceptScore W2145483252C158711907 @default.
• W2145483252 hasConceptScore W2145483252C16613235 @default.
• W2145483252 hasConceptScore W2145483252C178790620 @default.
• W2145483252 hasConceptScore W2145483252C181199279 @default.
• W2145483252 hasConceptScore W2145483252C185592680 @default.
• W2145483252 hasConceptScore W2145483252C2776901170 @default.
• W2145483252 hasConceptScore W2145483252C2779133857 @default.
• W2145483252 hasConceptScore W2145483252C28859421 @default.
• W2145483252 hasConceptScore W2145483252C537181965 @default.
• W2145483252 hasConceptScore W2145483252C55493867 @default.
• W2145483252 hasConceptScore W2145483252C57600042 @default.
• W2145483252 hasConceptScore W2145483252C62231903 @default.
• W2145483252 hasConceptScore W2145483252C62520636 @default.

• next