Matches in SemOpenAlex for { <https://semopenalex.org/work/W2145483420> ?p ?o ?g. }
- W2145483420 endingPage "5077" @default.
- W2145483420 startingPage "5067" @default.
- W2145483420 abstract "Abstract Most phosphoproteomic studies to date have been limited to the identification of phosphoproteins and their phosphorylation sites, and have not assessed the stoichiometry of protein phosphorylation, a critical parameter reflecting the dynamic equilibrium between phosphorylated and non‐phosphorylated pools of proteins. Here, we used a method for measuring phosphorylation stoichiometry through isotope tagging and enzymatic dephosphorylation of tryptic peptides. Using this method, protein digests are divided into two equal aliquots that are modified with either light or heavy isotope tags. One aliquot is dephosphorylated by alkaline phosphatase. Finally, the peptide mixtures are recombined and LC‐MS/MS analysis is performed. With this method, we studied adipocytes of mice stimulated with CL316,243, a β‐3 adrenergic agonist known to induce lipolysis and marked phosphorylation changes in proteins of the lipid droplet surface. In lipid droplet preparations, CL316,243 administration increased phosphorylation of proteins related to regulation of signaling, metabolism and intracellular trafficking in white adipose tissue, including hormone‐sensitive lipase which was 80% phosphorylated at the previously reported site, Ser‐559, and the lipid surface protein perilipin, which was phosphorylated by ∼60 and ∼40% at previously unreported sites, Ser‐410 and Ser‐460." @default.
- W2145483420 created "2016-06-24" @default.
- W2145483420 creator A5011067501 @default.
- W2145483420 creator A5024805251 @default.
- W2145483420 creator A5046766740 @default.
- W2145483420 creator A5047910628 @default.
- W2145483420 creator A5049088657 @default.
- W2145483420 creator A5069269715 @default.
- W2145483420 creator A5075555194 @default.
- W2145483420 date "2009-11-01" @default.
- W2145483420 modified "2023-10-10" @default.
- W2145483420 title "The stoichiometry of protein phosphorylation in adipocyte lipid droplets: Analysis by N-terminal isotope tagging and enzymatic dephosphorylation" @default.
- W2145483420 cites W1555247363 @default.
- W2145483420 cites W1575714113 @default.
- W2145483420 cites W1579554871 @default.
- W2145483420 cites W1945140151 @default.
- W2145483420 cites W1963719517 @default.
- W2145483420 cites W1965039157 @default.
- W2145483420 cites W1966723744 @default.
- W2145483420 cites W1974560613 @default.
- W2145483420 cites W1982136807 @default.
- W2145483420 cites W1986810516 @default.
- W2145483420 cites W1993465904 @default.
- W2145483420 cites W2019858779 @default.
- W2145483420 cites W2019981157 @default.
- W2145483420 cites W2020377122 @default.
- W2145483420 cites W2021185979 @default.
- W2145483420 cites W2026654700 @default.
- W2145483420 cites W2030170677 @default.
- W2145483420 cites W2031502981 @default.
- W2145483420 cites W2032708781 @default.
- W2145483420 cites W2038163496 @default.
- W2145483420 cites W2046333439 @default.
- W2145483420 cites W2053150532 @default.
- W2145483420 cites W2059275312 @default.
- W2145483420 cites W2061795728 @default.
- W2145483420 cites W2062263277 @default.
- W2145483420 cites W2065794292 @default.
- W2145483420 cites W2065902723 @default.
- W2145483420 cites W2068481474 @default.
- W2145483420 cites W2070391349 @default.
- W2145483420 cites W2072389876 @default.
- W2145483420 cites W2075045273 @default.
- W2145483420 cites W2086086273 @default.
- W2145483420 cites W2089971098 @default.
- W2145483420 cites W2095478277 @default.
- W2145483420 cites W2099255819 @default.
- W2145483420 cites W2100899036 @default.
- W2145483420 cites W2101543956 @default.
- W2145483420 cites W2112181124 @default.
- W2145483420 cites W2113317108 @default.
- W2145483420 cites W2119598335 @default.
- W2145483420 cites W2121253965 @default.
- W2145483420 cites W2134886125 @default.
- W2145483420 cites W2148662420 @default.
- W2145483420 cites W2154281855 @default.
- W2145483420 cites W2155880596 @default.
- W2145483420 cites W2165923048 @default.
- W2145483420 cites W2166446773 @default.
- W2145483420 cites W2166872160 @default.
- W2145483420 cites W2790360897 @default.
- W2145483420 cites W813270164 @default.
- W2145483420 doi "https://doi.org/10.1002/pmic.200800861" @default.
- W2145483420 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19921680" @default.
- W2145483420 hasPublicationYear "2009" @default.
- W2145483420 type Work @default.
- W2145483420 sameAs 2145483420 @default.
- W2145483420 citedByCount "21" @default.
- W2145483420 countsByYear W21454834202012 @default.
- W2145483420 countsByYear W21454834202013 @default.
- W2145483420 countsByYear W21454834202015 @default.
- W2145483420 countsByYear W21454834202016 @default.
- W2145483420 countsByYear W21454834202017 @default.
- W2145483420 countsByYear W21454834202018 @default.
- W2145483420 countsByYear W21454834202020 @default.
- W2145483420 countsByYear W21454834202022 @default.
- W2145483420 countsByYear W21454834202023 @default.
- W2145483420 crossrefType "journal-article" @default.
- W2145483420 hasAuthorship W2145483420A5011067501 @default.
- W2145483420 hasAuthorship W2145483420A5024805251 @default.
- W2145483420 hasAuthorship W2145483420A5046766740 @default.
- W2145483420 hasAuthorship W2145483420A5047910628 @default.
- W2145483420 hasAuthorship W2145483420A5049088657 @default.
- W2145483420 hasAuthorship W2145483420A5069269715 @default.
- W2145483420 hasAuthorship W2145483420A5075555194 @default.
- W2145483420 hasConcept C11960822 @default.
- W2145483420 hasConcept C140985366 @default.
- W2145483420 hasConcept C171089720 @default.
- W2145483420 hasConcept C178666793 @default.
- W2145483420 hasConcept C181912034 @default.
- W2145483420 hasConcept C185592680 @default.
- W2145483420 hasConcept C25095133 @default.
- W2145483420 hasConcept C2776175234 @default.
- W2145483420 hasConcept C2776414213 @default.
- W2145483420 hasConcept C2779281246 @default.
- W2145483420 hasConcept C2781227529 @default.
- W2145483420 hasConcept C39567582 @default.
- W2145483420 hasConcept C4733338 @default.