FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2145645462> ?p ?o ?g. }

• W2145645462 abstract "A major goal in signaling biology is the establishment of high-throughput quantitative methods for measuring changes in protein phosphorylation of entire signal transduction pathways across many different samples comprising temporal or dose data or patient samples. Data-independent acquisition (DIA) mass spectrometry (MS) methods, which involve tandem MS scans that are collected independently of precursor ion information and then are followed by targeted searching for known peptides, may achieve this goal. We applied DIA-MS to systematically quantify phosphorylation of components in the insulin signaling network in response to insulin as well as in stimulated cells exposed to a panel of kinase inhibitors targeting key downstream effectors in the network. We accurately quantified the effect of insulin on phosphorylation of 86 protein targets in the insulin signaling network using either stable isotope standards (SIS) or label-free quantification (LFQ) and mapped signal transmission through this network. By matching kinases to specific phosphorylation events (based on linear consensus motifs and temporal phosphorylation) to the quantitative phosphoproteomic data from cells exposed to inhibitors, we investigated predicted kinase-substrate relationships of AKT and mTOR in a targeted fashion. Furthermore, we applied this approach to show that AKT2-dependent phosphorylation of GAB2 promoted insulin signaling but inhibited epidermal growth factor (EGF) signaling in a manner dependent on 14-3-3 binding. Because DIA-MS can increase throughput and improve the reproducibility of peptide detection across multiple samples, this approach should facilitate more accurate, comprehensive, and quantitative assessment of signaling networks under various experimental conditions than are possible using other MS proteomic methods." @default.
• W2145645462 created "2016-06-24" @default.
• W2145645462 creator A5014643889 @default.
• W2145645462 creator A5023989691 @default.
• W2145645462 creator A5026046629 @default.
• W2145645462 creator A5031502585 @default.
• W2145645462 creator A5051169332 @default.
• W2145645462 creator A5052345144 @default.
• W2145645462 creator A5062542643 @default.
• W2145645462 date "2015-06-09" @default.
• W2145645462 modified "2023-10-01" @default.
• W2145645462 title "Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry" @default.
• W2145645462 cites W1803908051 @default.
• W2145645462 cites W1964328150 @default.
• W2145645462 cites W1964434806 @default.
• W2145645462 cites W1965030222 @default.
• W2145645462 cites W1965807926 @default.
• W2145645462 cites W1989833877 @default.
• W2145645462 cites W1995403127 @default.
• W2145645462 cites W1995478292 @default.
• W2145645462 cites W1996768724 @default.
• W2145645462 cites W1998295756 @default.
• W2145645462 cites W2001934796 @default.
• W2145645462 cites W2016267237 @default.
• W2145645462 cites W2018126724 @default.
• W2145645462 cites W2053943711 @default.
• W2145645462 cites W2062143232 @default.
• W2145645462 cites W2064631610 @default.
• W2145645462 cites W2073371472 @default.
• W2145645462 cites W2075669011 @default.
• W2145645462 cites W2079843936 @default.
• W2145645462 cites W2083990797 @default.
• W2145645462 cites W2086900521 @default.
• W2145645462 cites W2087331879 @default.
• W2145645462 cites W2093255461 @default.
• W2145645462 cites W2099368278 @default.
• W2145645462 cites W2101771026 @default.
• W2145645462 cites W2107710616 @default.
• W2145645462 cites W2117028091 @default.
• W2145645462 cites W2120432194 @default.
• W2145645462 cites W2128031393 @default.
• W2145645462 cites W2141091417 @default.
• W2145645462 cites W2143340505 @default.
• W2145645462 cites W2152095891 @default.
• W2145645462 cites W2319494906 @default.
• W2145645462 cites W2325375737 @default.
• W2145645462 doi "https://doi.org/10.1126/scisignal.aaa3139" @default.
• W2145645462 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26060331" @default.
• W2145645462 hasPublicationYear "2015" @default.
• W2145645462 type Work @default.
• W2145645462 sameAs 2145645462 @default.
• W2145645462 citedByCount "52" @default.
• W2145645462 countsByYear W21456454622015 @default.
• W2145645462 countsByYear W21456454622016 @default.
• W2145645462 countsByYear W21456454622017 @default.
• W2145645462 countsByYear W21456454622018 @default.
• W2145645462 countsByYear W21456454622019 @default.
• W2145645462 countsByYear W21456454622020 @default.
• W2145645462 countsByYear W21456454622021 @default.
• W2145645462 countsByYear W21456454622022 @default.
• W2145645462 countsByYear W21456454622023 @default.
• W2145645462 crossrefType "journal-article" @default.
• W2145645462 hasAuthorship W2145645462A5014643889 @default.
• W2145645462 hasAuthorship W2145645462A5023989691 @default.
• W2145645462 hasAuthorship W2145645462A5026046629 @default.
• W2145645462 hasAuthorship W2145645462A5031502585 @default.
• W2145645462 hasAuthorship W2145645462A5051169332 @default.
• W2145645462 hasAuthorship W2145645462A5052345144 @default.
• W2145645462 hasAuthorship W2145645462A5062542643 @default.
• W2145645462 hasConcept C104317684 @default.
• W2145645462 hasConcept C112446052 @default.
• W2145645462 hasConcept C11960822 @default.
• W2145645462 hasConcept C134018914 @default.
• W2145645462 hasConcept C184235292 @default.
• W2145645462 hasConcept C2777391703 @default.
• W2145645462 hasConcept C2779306644 @default.
• W2145645462 hasConcept C2909831755 @default.
• W2145645462 hasConcept C46111723 @default.
• W2145645462 hasConcept C55493867 @default.
• W2145645462 hasConcept C62478195 @default.
• W2145645462 hasConcept C6675166 @default.
• W2145645462 hasConcept C70721500 @default.
• W2145645462 hasConcept C75217442 @default.
• W2145645462 hasConcept C80311884 @default.
• W2145645462 hasConcept C86554907 @default.
• W2145645462 hasConcept C86803240 @default.
• W2145645462 hasConcept C87325107 @default.
• W2145645462 hasConcept C95444343 @default.
• W2145645462 hasConcept C97029542 @default.
• W2145645462 hasConceptScore W2145645462C104317684 @default.
• W2145645462 hasConceptScore W2145645462C112446052 @default.
• W2145645462 hasConceptScore W2145645462C11960822 @default.
• W2145645462 hasConceptScore W2145645462C134018914 @default.
• W2145645462 hasConceptScore W2145645462C184235292 @default.
• W2145645462 hasConceptScore W2145645462C2777391703 @default.
• W2145645462 hasConceptScore W2145645462C2779306644 @default.
• W2145645462 hasConceptScore W2145645462C2909831755 @default.
• W2145645462 hasConceptScore W2145645462C46111723 @default.
• W2145645462 hasConceptScore W2145645462C55493867 @default.
• W2145645462 hasConceptScore W2145645462C62478195 @default.

• next