FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2145688645> ?p ?o ?g. }

• W2145688645 endingPage "505" @default.
• W2145688645 startingPage "493" @default.
• W2145688645 abstract "Nep1-like proteins (NLPs), produced by an array of unrelated microorganisms, are phytotoxic for dicotyledonous plant cells but their mode of action has not yet been established. Two paralogous NLPs from the necrotrophic plant pathogenic fungus Botrytis cinerea were characterized, designated BcNEP1 and BcNEP2. Both proteins were produced in the heterologous host Pichia pastoris and purified to homogeneity. The localization of fluorescently labelled proteins was studied and mechanisms of cell death were investigated in protoplasts and suspension cells. Purified BcNEP1 and BcNEP2 caused necrosis in all dicotyledonous plant species tested, but not in monocotyledons. A synthetic heptapeptide comprising a sequence (GHRHDWE) that is conserved in all NLPs did not cause symptoms and was unable to interfere with necrosis induction by BcNEP1 and BcNEP2 proteins. Fluorescently labelled BcNEP1 and BcNEP2 proteins were associated with plasma membranes and the nuclear envelope, as well as in the nucleolus of responding plant cells. A strong hydrogen peroxide (H(2)O(2)) accumulation was observed in chloroplasts. The death process was characterized by TUNEL assays as apoptosis, necrosis or intermediate forms of both. BcNEP1- and BcNEP2-induced cell death execution could not be abolished by specific inhibitors. These results provide further information on mechanisms of NLP-inflicted cell death." @default.
• W2145688645 created "2016-06-24" @default.
• W2145688645 creator A5052918525 @default.
• W2145688645 creator A5056744077 @default.
• W2145688645 creator A5088616450 @default.
• W2145688645 date "2007-11-19" @default.
• W2145688645 modified "2023-09-26" @default.
• W2145688645 title "Phytotoxic Nep1‐like proteins from the necrotrophic fungus <i>Botrytis cinerea</i> associate with membranes and the nucleus of plant cells" @default.
• W2145688645 cites W1502411301 @default.
• W2145688645 cites W1600487128 @default.
• W2145688645 cites W1964313477 @default.
• W2145688645 cites W1979096677 @default.
• W2145688645 cites W2002218478 @default.
• W2145688645 cites W2004314009 @default.
• W2145688645 cites W2018348440 @default.
• W2145688645 cites W2025117402 @default.
• W2145688645 cites W2046593879 @default.
• W2145688645 cites W2049856484 @default.
• W2145688645 cites W2059925042 @default.
• W2145688645 cites W2061117777 @default.
• W2145688645 cites W2066237635 @default.
• W2145688645 cites W2067431766 @default.
• W2145688645 cites W2071801786 @default.
• W2145688645 cites W2074018114 @default.
• W2145688645 cites W2079297094 @default.
• W2145688645 cites W2080483833 @default.
• W2145688645 cites W2100194182 @default.
• W2145688645 cites W2100837269 @default.
• W2145688645 cites W2103010275 @default.
• W2145688645 cites W2104244040 @default.
• W2145688645 cites W2106917466 @default.
• W2145688645 cites W2107537542 @default.
• W2145688645 cites W2108891562 @default.
• W2145688645 cites W2111764142 @default.
• W2145688645 cites W2115772647 @default.
• W2145688645 cites W2116511611 @default.
• W2145688645 cites W2117184453 @default.
• W2145688645 cites W2118127427 @default.
• W2145688645 cites W2121355629 @default.
• W2145688645 cites W2121615663 @default.
• W2145688645 cites W2124544231 @default.
• W2145688645 cites W2125600358 @default.
• W2145688645 cites W2133669258 @default.
• W2145688645 cites W2139456772 @default.
• W2145688645 cites W2142895701 @default.
• W2145688645 cites W2145100181 @default.
• W2145688645 cites W2146615689 @default.
• W2145688645 cites W2152643698 @default.
• W2145688645 cites W2158808730 @default.
• W2145688645 cites W2161305805 @default.
• W2145688645 cites W2161746138 @default.
• W2145688645 cites W2164276984 @default.
• W2145688645 cites W2169776797 @default.
• W2145688645 cites W2169953844 @default.
• W2145688645 cites W2951239624 @default.
• W2145688645 cites W3023731605 @default.
• W2145688645 doi "https://doi.org/10.1111/j.1469-8137.2007.02274.x" @default.
• W2145688645 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18028294" @default.
• W2145688645 hasPublicationYear "2007" @default.
• W2145688645 type Work @default.
• W2145688645 sameAs 2145688645 @default.
• W2145688645 citedByCount "109" @default.
• W2145688645 countsByYear W21456886452012 @default.
• W2145688645 countsByYear W21456886452013 @default.
• W2145688645 countsByYear W21456886452014 @default.
• W2145688645 countsByYear W21456886452015 @default.
• W2145688645 countsByYear W21456886452016 @default.
• W2145688645 countsByYear W21456886452017 @default.
• W2145688645 countsByYear W21456886452018 @default.
• W2145688645 countsByYear W21456886452019 @default.
• W2145688645 countsByYear W21456886452020 @default.
• W2145688645 countsByYear W21456886452021 @default.
• W2145688645 countsByYear W21456886452022 @default.
• W2145688645 countsByYear W21456886452023 @default.
• W2145688645 crossrefType "journal-article" @default.
• W2145688645 hasAuthorship W2145688645A5052918525 @default.
• W2145688645 hasAuthorship W2145688645A5056744077 @default.
• W2145688645 hasAuthorship W2145688645A5088616450 @default.
• W2145688645 hasConcept C2775867217 @default.
• W2145688645 hasConcept C2779678110 @default.
• W2145688645 hasConcept C2780723820 @default.
• W2145688645 hasConcept C41625074 @default.
• W2145688645 hasConcept C55493867 @default.
• W2145688645 hasConcept C59822182 @default.
• W2145688645 hasConcept C86803240 @default.
• W2145688645 hasConcept C95444343 @default.
• W2145688645 hasConceptScore W2145688645C2775867217 @default.
• W2145688645 hasConceptScore W2145688645C2779678110 @default.
• W2145688645 hasConceptScore W2145688645C2780723820 @default.
• W2145688645 hasConceptScore W2145688645C41625074 @default.
• W2145688645 hasConceptScore W2145688645C55493867 @default.
• W2145688645 hasConceptScore W2145688645C59822182 @default.
• W2145688645 hasConceptScore W2145688645C86803240 @default.
• W2145688645 hasConceptScore W2145688645C95444343 @default.
• W2145688645 hasIssue "2" @default.
• W2145688645 hasLocation W21456886451 @default.
• W2145688645 hasOpenAccess W2145688645 @default.
• W2145688645 hasPrimaryLocation W21456886451 @default.

• next