FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2145697300> ?p ?o ?g. }

• W2145697300 endingPage "208" @default.
• W2145697300 startingPage "197" @default.
• W2145697300 abstract "A soft-sensor based on an Extended Kalman Filter (EKF) that combines data obtained using a fluorescence-based soft-sensor with a dynamic mechanistic model, was investigated as a tool for continuous monitoring of a Chinese hamster ovary (CHO) cell cultivation process. A standalone fluorescence based soft-sensor, which uses a combination of an empirical multivariate statistical model and measured spectra, was designed for predicting key culture variables including viable and dead cells, recombinant protein, glucose, and ammonia concentrations. The standalone fluorescence sensor was then combined with a dynamic mechanistic model within an EKF framework, for improving the prediction accuracy and generating predictions in-between sampling instances. The dynamic model used for the EKF framework was based on a structured metabolic flux analysis and mass balances. In order to calibrate the fluorescence-based empirical model and the dynamic mechanistic model, cells were grown in batch mode with different initial glucose and glutamine concentrations. To mitigate the uncertainty associated with the model structure and parameters, non-stationary disturbances were accounted for in the EKF by parameter-adaptation. It was demonstrated that the implementation of the EKF along with the dynamic model could improve the accuracy of the fluorescence-based predictions at the sampling instances. Additionally, it was shown that the major advantage of the EKF-based soft-sensor, compared to the standalone fluorescence-based counterpart, was its capability to track the temporal evolution of key process variables between measurement instances obtained by the fluorescence-based soft-sensor. This is crucial for designing control strategies of CHO cell cultures with the aim of guaranteeing product quality. Biotechnol. Bioeng. 2015;112: 197–208. © 2014 Wiley Periodicals, Inc." @default.
• W2145697300 created "2016-06-24" @default.
• W2145697300 creator A5009713991 @default.
• W2145697300 creator A5044146535 @default.
• W2145697300 creator A5072957228 @default.
• W2145697300 date "2014-09-26" @default.
• W2145697300 modified "2023-10-17" @default.
• W2145697300 title "Development of a soft-sensor based on multi-wavelength fluorescence spectroscopy and a dynamic metabolic model for monitoring mammalian cell cultures" @default.
• W2145697300 cites W1906763645 @default.
• W2145697300 cites W1915353445 @default.
• W2145697300 cites W1964257826 @default.
• W2145697300 cites W1976208842 @default.
• W2145697300 cites W1976251851 @default.
• W2145697300 cites W1977675094 @default.
• W2145697300 cites W1978244947 @default.
• W2145697300 cites W1990597832 @default.
• W2145697300 cites W1997965813 @default.
• W2145697300 cites W1998586062 @default.
• W2145697300 cites W2000651380 @default.
• W2145697300 cites W2009127955 @default.
• W2145697300 cites W2010186050 @default.
• W2145697300 cites W2021343643 @default.
• W2145697300 cites W2026546169 @default.
• W2145697300 cites W2031556861 @default.
• W2145697300 cites W2037435747 @default.
• W2145697300 cites W2039536734 @default.
• W2145697300 cites W2057328981 @default.
• W2145697300 cites W2064928584 @default.
• W2145697300 cites W2065960941 @default.
• W2145697300 cites W2067338244 @default.
• W2145697300 cites W2069349248 @default.
• W2145697300 cites W2071646337 @default.
• W2145697300 cites W2075368931 @default.
• W2145697300 cites W2121414985 @default.
• W2145697300 cites W2138715996 @default.
• W2145697300 cites W2143177819 @default.
• W2145697300 cites W2158863190 @default.
• W2145697300 cites W2163754616 @default.
• W2145697300 doi "https://doi.org/10.1002/bit.25339" @default.
• W2145697300 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25065633" @default.
• W2145697300 hasPublicationYear "2014" @default.
• W2145697300 type Work @default.
• W2145697300 sameAs 2145697300 @default.
• W2145697300 citedByCount "33" @default.
• W2145697300 countsByYear W21456973002015 @default.
• W2145697300 countsByYear W21456973002016 @default.
• W2145697300 countsByYear W21456973002017 @default.
• W2145697300 countsByYear W21456973002018 @default.
• W2145697300 countsByYear W21456973002019 @default.
• W2145697300 countsByYear W21456973002020 @default.
• W2145697300 countsByYear W21456973002021 @default.
• W2145697300 countsByYear W21456973002022 @default.
• W2145697300 countsByYear W21456973002023 @default.
• W2145697300 crossrefType "journal-article" @default.
• W2145697300 hasAuthorship W2145697300A5009713991 @default.
• W2145697300 hasAuthorship W2145697300A5044146535 @default.
• W2145697300 hasAuthorship W2145697300A5072957228 @default.
• W2145697300 hasConcept C106131492 @default.
• W2145697300 hasConcept C111919701 @default.
• W2145697300 hasConcept C115575686 @default.
• W2145697300 hasConcept C140779682 @default.
• W2145697300 hasConcept C154945302 @default.
• W2145697300 hasConcept C157286648 @default.
• W2145697300 hasConcept C170493617 @default.
• W2145697300 hasConcept C175656101 @default.
• W2145697300 hasConcept C185592680 @default.
• W2145697300 hasConcept C186060115 @default.
• W2145697300 hasConcept C206833254 @default.
• W2145697300 hasConcept C31972630 @default.
• W2145697300 hasConcept C41008148 @default.
• W2145697300 hasConcept C55493867 @default.
• W2145697300 hasConcept C86803240 @default.
• W2145697300 hasConcept C98045186 @default.
• W2145697300 hasConceptScore W2145697300C106131492 @default.
• W2145697300 hasConceptScore W2145697300C111919701 @default.
• W2145697300 hasConceptScore W2145697300C115575686 @default.
• W2145697300 hasConceptScore W2145697300C140779682 @default.
• W2145697300 hasConceptScore W2145697300C154945302 @default.
• W2145697300 hasConceptScore W2145697300C157286648 @default.
• W2145697300 hasConceptScore W2145697300C170493617 @default.
• W2145697300 hasConceptScore W2145697300C175656101 @default.
• W2145697300 hasConceptScore W2145697300C185592680 @default.
• W2145697300 hasConceptScore W2145697300C186060115 @default.
• W2145697300 hasConceptScore W2145697300C206833254 @default.
• W2145697300 hasConceptScore W2145697300C31972630 @default.
• W2145697300 hasConceptScore W2145697300C41008148 @default.
• W2145697300 hasConceptScore W2145697300C55493867 @default.
• W2145697300 hasConceptScore W2145697300C86803240 @default.
• W2145697300 hasConceptScore W2145697300C98045186 @default.
• W2145697300 hasIssue "1" @default.
• W2145697300 hasLocation W21456973001 @default.
• W2145697300 hasLocation W21456973002 @default.
• W2145697300 hasOpenAccess W2145697300 @default.
• W2145697300 hasPrimaryLocation W21456973001 @default.
• W2145697300 hasRelatedWork W119418178 @default.
• W2145697300 hasRelatedWork W2019099201 @default.
• W2145697300 hasRelatedWork W2103062922 @default.
• W2145697300 hasRelatedWork W2145697300 @default.

• next