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- W2145890677 abstract "Central to many molecular biology techniques as ubiquitous as PCR and Southern blotting is the design of oligonucleotide (oligo) probes and/or primers possessing specific thermodynamic properties. Here, we use validated theoretical methods to generate distributions of predicted thermodynamic properties for DNA oligos of various lengths. These distributions facilitate immediate appreciation of typical thermodynamic values for oligos of various lengths.Distributions of melting temperature (Tm), free energy (DeltaG(T)o), and fraction hybridized or fraction bound (Fb), are presented for oligos of length 10-50 bases sampled from the human genome. The effects of changing temperature, oligo and salt concentrations, constraining G+C content, and introducing mismatches are exemplified. Our results provide the first survey of typical and limiting thermodynamic values evaluated on a genomic scale. Described numbers comprise useful 'rules of thumb' that are applicable to most technologies dependent upon DNA oligo design." @default.
- W2145890677 created "2016-06-24" @default.
- W2145890677 creator A5000468509 @default.
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- W2145890677 date "2005-06-09" @default.
- W2145890677 modified "2023-10-10" @default.
- W2145890677 title "Thermodynamic properties of DNA sequences: characteristic values for the human genome" @default.
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- W2145890677 doi "https://doi.org/10.1093/bioinformatics/bti530" @default.
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