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• W2145967173 abstract "Abstract Comparative genomic analyses of Candida glabrata and Saccharomyces cerevisiae suggest many signal transduction pathways are highly conserved. Focusing on the phosphate signal transduction (PHO) pathway of C. glabrata, we demonstrate that components of the pathway are conserved and confirm the role of CgPHO81, CgPHO80, CgPHO4, and CgMSN5 in the PHO pathway through deletion analysis. Unlike S. cerevisiae, C. glabrata shows little dependence on the transcription factor, Pho2, for induction of phosphate-regulated genes during phosphate limitation. We show that the CgPho4 protein is necessary and sufficient for Pho2-independent gene expression; CgPho4 is capable of driving expression of PHO promoters in S. cerevisiae in the absence of ScPHO2. On the basis of the sequences of PHO4 in the hemiascomycetes and complementation analysis, we suggest that Pho2 dependence is a trait only observed in species closely related to S. cerevisiae. Our data are consistent with trans-regulatory changes in the PHO pathway via the transcription factor Pho4 as opposed to cis-regulatory changes (the promoter)." @default.
• W2145967173 created "2016-06-24" @default.
• W2145967173 creator A5035809948 @default.
• W2145967173 creator A5056630573 @default.
• W2145967173 date "2009-06-01" @default.
• W2145967173 modified "2023-10-18" @default.
• W2145967173 title "<i>Candida glabrata PHO4</i> Is Necessary and Sufficient for Pho2-Independent Transcription of Phosphate Starvation Genes" @default.
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• W2145967173 doi "https://doi.org/10.1534/genetics.109.101063" @default.
• W2145967173 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2691756" @default.
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