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- W2145969305 abstract "A woman with recurrent Paget's disease of the vulva developed acute graft-versus-host disease (GVHD) 12 days after radical surgery and massive blood transfusion. Molecular diagnosis of lymphocyte chimerism in the peripheral blood was made by polymerase chain reaction (PCR) directed against a Y chromosome-specific sex-determining region Y (SRY) gene. PCR with skin biopsy after onset of GVHD also revealed infiltration of SRY-positive donor lymphocytes. The diagnosis was confirmed by HLA-DNA typing with PCR-sequence-specific oligonucleotide that revealed the presence of complex HLA-DR chimerism in the peripheral lymphocytes collected after onset of GVHD. The use of SRY- directed PCR is a rapid technique for the early diagnosis of acute posttransfusion GVHD in female patients." @default.
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- W2145969305 date "1993-05-01" @default.
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- W2145969305 title "A rapid molecular diagnosis of posttransfusion graft-versus-host disease by polymerase chain reaction" @default.
- W2145969305 doi "https://doi.org/10.1046/j.1537-2995.1993.33593255603.x" @default.
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