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- W2146024897 abstract "ABSTRACT In contrast to other retroviruses, Mason-Pfizer monkey virus (M-PMV) assembles immature capsids in the cytoplasm. We have compared the ability of minimal assembly-competent domains from M-PMV and human immunodeficiency virus type 1 (HIV-1) to assemble in vitro into virus-like particles in the presence and absence of nucleic acids. A fusion protein comprised of the capsid and nucleocapsid domains of Gag (CANC) and its N-terminally modified mutant (ΔProCANC) were used to mimic the assembly of the viral core and immature particles, respectively. In contrast to HIV-1, where CANC assembled efficiently into cylindrical structures, the same domains of M-PMV were assembly incompetent. The addition of RNA or oligonucleotides did not complement this defect. In contrast, the M-PMV ΔProCANC molecule was able to assemble into spherical particles, while that of HIV-1 formed both spheres and cylinders. For M-PMV, the addition of purified RNA increased the efficiency with which ΔProCANC formed spherical particles both in terms of the overall amount and the numbers of completed spheres. The amount of RNA incorporated was determined, and for both rRNA and MS2-RNA, quantities similar to that of genomic RNA were encapsidated. Oligonucleotides also stimulated assembly; however, they were incorporated into ΔProCANC spherical particles in trace amounts that could not serve as a stoichiometric structural component for assembly. Thus, oligonucleotides may, through a transient interaction, induce conformational changes that facilitate assembly, while longer RNAs appear to facilitate the complete assembly of spherical particles." @default.
- W2146024897 created "2016-06-24" @default.
- W2146024897 creator A5004793292 @default.
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- W2146024897 creator A5057667007 @default.
- W2146024897 creator A5062613922 @default.
- W2146024897 creator A5082313921 @default.
- W2146024897 date "2006-07-15" @default.
- W2146024897 modified "2023-10-02" @default.
- W2146024897 title "Distinct Roles for Nucleic Acid in In Vitro Assembly of Purified Mason-Pfizer Monkey Virus CANC Proteins" @default.
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- W2146024897 doi "https://doi.org/10.1128/jvi.02694-05" @default.
- W2146024897 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1489063" @default.
- W2146024897 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16809314" @default.
- W2146024897 hasPublicationYear "2006" @default.
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