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- W2146098573 abstract "The association between the rate of protein degradation and the components of the calpain system in lambs fed at sub-maintenance, maintenance and supra-maintenance levels of nutrition was investigated. Weights of the cold carcass, liver, kidney, pluck and m. semitendinosus increased with nutritional level ( P <0·05). The rate of protein degradation in the hind-limb was determined using an in vivo arterio-venous method. Blood flow, protein gain and protein synthesis across the hind-limb increased with nutritional status ( P <0·05). There was an increase in the amount of protein synthesised per unit of RNA (mg RNA/g protein) with improved nutritional status. The rate of protein degradation across the hind-limb increased between the sub-maintenance and maintenance treatments ( P <0·05) but there was no further increase above maintenance ( P >0·10). The activity of the components of the calpain system was determined after separation on a DEAE-Sepharose column with a stepwise gradient with increasing NaCl concentrations. Although there were no significant effects of nutritional status on the components of the calpain system, there was a negative association between the rate of protein degradation and the activity of μ-calpain ( R 2 =0·61; P <0·005) and a weak positive association between calpastatin activity and protein gain ( R 2 =0·44; P <0·05)." @default.
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- W2146098573 date "1997-12-01" @default.
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- W2146098573 title "The effect of nutritional status on protein degradation and components of the calpain system in skeletal muscle of weaned wether lambs" @default.
- W2146098573 doi "https://doi.org/10.1017/s0021859697004784" @default.
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