FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2146168510> ?p ?o ?g. }

• W2146168510 endingPage "2787" @default.
• W2146168510 startingPage "2777" @default.
• W2146168510 abstract "We previously reported the development of an in vitro adeno-associated virus (AAV) DNA replication system. The system required one of the p5 Rep proteins encoded by AAV (either Rep78 or Rep68) and a crude adenovirus (Ad)-infected HeLa cell cytoplasmic extract to catalyze origin of replication-dependent AAV DNA replication. However, in addition to fully permissive DNA replication, which occurs in the presence of Ad, AAV is also capable of partially permissive DNA replication in the absence of the helper virus in cells that have been treated with genotoxic agents. Limited DNA replication also occurs in the absence of Ad during the process of establishing a latent infection. In an attempt to isolate uninfected extracts that would support AAV DNA replication, we discovered that HeLa cell extracts grown to high density can occasionally display as much in vitro replication activity as Ad-infected extracts. This finding confirmed previous genetic analyses which suggested that no Ad-encoded proteins were absolutely essential for AAV DNA replication and that the uninfected extracts should be useful for studying the differences between helper-dependent and helper-independent AAV DNA replication. Using specific chemical inhibitors and monoclonal antibodies, as well as the fractionation of uninfected HeLa extracts, we identified several of the cellular enzymes involved in AAV DNA replication. They were the single-stranded DNA binding protein, replication protein A (RFA), the 3' primer binding complex, replication factor C (RFC), and proliferating cell nuclear antigen (PCNA). Consistent with the current model for AAV DNA replication, which requires only leading-strand DNA synthesis, we found no requirement for DNA polymerase alpha-primase. AAV DNA replication could be reconstituted with purified Rep78, RPA, RFC, and PCNA and a phosphocellulose chromatography fraction (IIA) that contained DNA polymerase activity. As both RFC and PCNA are known to be accessory proteins for polymerase delta and epsilon, we attempted to reconstitute AAV DNA replication by substituting either purified polymerase delta or polymerase epsilon for fraction IIA. These attempts were unsuccessful and suggested that some novel cellular protein or modification was required for AAV DNA replication that had not been previously identified. Finally, we also further characterized the in vitro DNA replication assay and demonstrated by two-dimensional (2D) gel electrophoresis that all of the intermediates commonly seen in vivo are generated in the in vitro system. The only difference was an accumulation of single-stranded DNA in vivo that was not seen in vitro. The 2D data also suggested that although both Rep78 and Rep68 can generate dimeric intermediates in vitro, Rep68 is more efficient in processing dimers to monomer duplex DNA. Regardless of the Rep that was used in vitro, we found evidence of an interaction between the elongation complex and the terminal repeats. Nicking at the terminal repeats of a replicating molecule appeared to be inhibited until after elongation was complete." @default.
• W2146168510 created "2016-06-24" @default.
• W2146168510 creator A5019848579 @default.
• W2146168510 creator A5024753271 @default.
• W2146168510 creator A5029365707 @default.
• W2146168510 creator A5071437258 @default.
• W2146168510 creator A5080734532 @default.
• W2146168510 creator A5085685711 @default.
• W2146168510 creator A5089661731 @default.
• W2146168510 date "1998-04-01" @default.
• W2146168510 modified "2023-10-10" @default.
• W2146168510 title "Cellular Proteins Required for Adeno-Associated Virus DNA Replication in the Absence of Adenovirus Coinfection" @default.
• W2146168510 cites W126651838 @default.
• W2146168510 cites W1500392367 @default.
• W2146168510 cites W1515990833 @default.
• W2146168510 cites W1516514485 @default.
• W2146168510 cites W1522993284 @default.
• W2146168510 cites W1527517879 @default.
• W2146168510 cites W1541189153 @default.
• W2146168510 cites W1544405851 @default.
• W2146168510 cites W1550656265 @default.
• W2146168510 cites W1552587525 @default.
• W2146168510 cites W1554104750 @default.
• W2146168510 cites W1556066808 @default.
• W2146168510 cites W1581008876 @default.
• W2146168510 cites W1589132235 @default.
• W2146168510 cites W1603686834 @default.
• W2146168510 cites W1605712430 @default.
• W2146168510 cites W1663294546 @default.
• W2146168510 cites W1699538093 @default.
• W2146168510 cites W1795219398 @default.
• W2146168510 cites W1807225431 @default.
• W2146168510 cites W1891666617 @default.
• W2146168510 cites W1900202044 @default.
• W2146168510 cites W1916339093 @default.
• W2146168510 cites W1920888136 @default.
• W2146168510 cites W1944079546 @default.
• W2146168510 cites W1967663075 @default.
• W2146168510 cites W1968382713 @default.
• W2146168510 cites W1985465535 @default.
• W2146168510 cites W1985810133 @default.
• W2146168510 cites W1987497828 @default.
• W2146168510 cites W2006303534 @default.
• W2146168510 cites W2008992593 @default.
• W2146168510 cites W2016782363 @default.
• W2146168510 cites W2020072111 @default.
• W2146168510 cites W2028043537 @default.
• W2146168510 cites W2029124757 @default.
• W2146168510 cites W2031083024 @default.
• W2146168510 cites W2033283534 @default.
• W2146168510 cites W2038149991 @default.
• W2146168510 cites W2048586948 @default.
• W2146168510 cites W2049900538 @default.
• W2146168510 cites W2050273056 @default.
• W2146168510 cites W2050285820 @default.
• W2146168510 cites W2051582515 @default.
• W2146168510 cites W2052858665 @default.
• W2146168510 cites W2059060156 @default.
• W2146168510 cites W2062909604 @default.
• W2146168510 cites W2063653562 @default.
• W2146168510 cites W2071223409 @default.
• W2146168510 cites W2075549762 @default.
• W2146168510 cites W2077214336 @default.
• W2146168510 cites W2082276980 @default.
• W2146168510 cites W2092713502 @default.
• W2146168510 cites W2095831047 @default.
• W2146168510 cites W2113808265 @default.
• W2146168510 cites W2118099969 @default.
• W2146168510 cites W2120491037 @default.
• W2146168510 cites W2122632939 @default.
• W2146168510 cites W2138552055 @default.
• W2146168510 cites W2139138981 @default.
• W2146168510 cites W2141359223 @default.
• W2146168510 cites W2141787155 @default.
• W2146168510 cites W2165181073 @default.
• W2146168510 cites W4211260918 @default.
• W2146168510 cites W4238175003 @default.
• W2146168510 cites W4247201266 @default.
• W2146168510 cites W838635636 @default.
• W2146168510 cites W997112341 @default.
• W2146168510 doi "https://doi.org/10.1128/jvi.72.4.2777-2787.1998" @default.
• W2146168510 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/109722" @default.
• W2146168510 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9525597" @default.
• W2146168510 hasPublicationYear "1998" @default.
• W2146168510 type Work @default.
• W2146168510 sameAs 2146168510 @default.
• W2146168510 citedByCount "93" @default.
• W2146168510 countsByYear W21461685102012 @default.
• W2146168510 countsByYear W21461685102013 @default.
• W2146168510 countsByYear W21461685102014 @default.
• W2146168510 countsByYear W21461685102015 @default.
• W2146168510 countsByYear W21461685102016 @default.
• W2146168510 countsByYear W21461685102017 @default.
• W2146168510 countsByYear W21461685102019 @default.
• W2146168510 countsByYear W21461685102020 @default.
• W2146168510 countsByYear W21461685102021 @default.
• W2146168510 countsByYear W21461685102022 @default.
• W2146168510 crossrefType "journal-article" @default.

• next