FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2146191961> ?p ?o ?g. }

• W2146191961 endingPage "456" @default.
• W2146191961 startingPage "448" @default.
• W2146191961 abstract "A simple one-step reverse transcription polymerase chain reaction (RT-PCR) method was developed based on T-rich insertions in the viral genome for simultaneous detection and differentiation of wild type and vaccine strains of Classical swine fever virus (CSFV). The CSFV-specific primers were designed to contain the sequences of the T-rich insertion sites that exist uniquely in the 3' nontranslated regions (3′ NTR) of the genome of lapinized CSFV vaccine strains. By using a one-step RT-PCR or a nested PCR followed by an agarose gel electrophoresis or a multicapillary electrophoresis, the wild-type and lapinized vaccine strains of CSFV in clinical samples could be detected and accurately distinguished. These assays can be applied to at least 3 attenuated lapinized vaccine strains, lapinized Philippines Coronel (LPC), hog cholera lapinized virus (HCLV), and Chinese strain (C strain). The detection limit of the wild-type virus was 6.3 TCID 50 (50% tissue culture infective dose)/ml for RT-PCR and 0.63 TCID 50 /ml for nested PCR. In previous studies, notable T-rich insertions of 12–13 nucleotides (nt) were found in the 3′ NTR of the genome of lapinized vaccine strains of CSFV. However, this study discovered that 2 T-rich insertions, 42 and 36 nt in length, are present in the viral genome of lapinized vaccine strains LPC/PRK (primary rabbit kidney) and LPC/TS (Tam-Sui), respectively. These T-rich insertions of 12, 36, and 42 nt length increases the size of PCR fragments, which are favorable genetic markers for rapid detection of and differentiation between wild-type and different lapinized vaccine strains of CSFV." @default.
• W2146191961 created "2016-06-24" @default.
• W2146191961 creator A5014767558 @default.
• W2146191961 creator A5015144381 @default.
• W2146191961 creator A5027652545 @default.
• W2146191961 creator A5033004114 @default.
• W2146191961 creator A5034779329 @default.
• W2146191961 creator A5051249476 @default.
• W2146191961 date "2008-07-01" @default.
• W2146191961 modified "2023-09-23" @default.
• W2146191961 title "Rapid Detection and Differentiation of Wild-Type and Three Attenuated Lapinized Vaccine Strains of <i>Classical Swine Fever Virus</i> by Reverse Transcription Polymerase Chain Reaction" @default.
• W2146191961 cites W1542576187 @default.
• W2146191961 cites W1608407313 @default.
• W2146191961 cites W1931343962 @default.
• W2146191961 cites W1992631461 @default.
• W2146191961 cites W2007302594 @default.
• W2146191961 cites W2024080387 @default.
• W2146191961 cites W2032233667 @default.
• W2146191961 cites W2045093985 @default.
• W2146191961 cites W2050013119 @default.
• W2146191961 cites W2061951215 @default.
• W2146191961 cites W2071416171 @default.
• W2146191961 cites W2074393814 @default.
• W2146191961 cites W2080106938 @default.
• W2146191961 cites W2097110814 @default.
• W2146191961 cites W2100707533 @default.
• W2146191961 cites W2106090606 @default.
• W2146191961 cites W2115200200 @default.
• W2146191961 cites W2132571290 @default.
• W2146191961 cites W2132679159 @default.
• W2146191961 cites W2137757503 @default.
• W2146191961 cites W2170488050 @default.
• W2146191961 cites W2323931425 @default.
• W2146191961 cites W2470593998 @default.
• W2146191961 cites W251278240 @default.
• W2146191961 cites W27431358 @default.
• W2146191961 cites W93736759 @default.
• W2146191961 doi "https://doi.org/10.1177/104063870802000406" @default.
• W2146191961 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18599849" @default.
• W2146191961 hasPublicationYear "2008" @default.
• W2146191961 type Work @default.
• W2146191961 sameAs 2146191961 @default.
• W2146191961 citedByCount "17" @default.
• W2146191961 countsByYear W21461919612012 @default.
• W2146191961 countsByYear W21461919612014 @default.
• W2146191961 countsByYear W21461919612016 @default.
• W2146191961 countsByYear W21461919612017 @default.
• W2146191961 countsByYear W21461919612020 @default.
• W2146191961 countsByYear W21461919612021 @default.
• W2146191961 crossrefType "journal-article" @default.
• W2146191961 hasAuthorship W2146191961A5014767558 @default.
• W2146191961 hasAuthorship W2146191961A5015144381 @default.
• W2146191961 hasAuthorship W2146191961A5027652545 @default.
• W2146191961 hasAuthorship W2146191961A5033004114 @default.
• W2146191961 hasAuthorship W2146191961A5034779329 @default.
• W2146191961 hasAuthorship W2146191961A5051249476 @default.
• W2146191961 hasConcept C104317684 @default.
• W2146191961 hasConcept C105580179 @default.
• W2146191961 hasConcept C141231307 @default.
• W2146191961 hasConcept C156719811 @default.
• W2146191961 hasConcept C159047783 @default.
• W2146191961 hasConcept C2522874641 @default.
• W2146191961 hasConcept C2777510210 @default.
• W2146191961 hasConcept C2778271344 @default.
• W2146191961 hasConcept C2778400998 @default.
• W2146191961 hasConcept C2779005923 @default.
• W2146191961 hasConcept C2780727368 @default.
• W2146191961 hasConcept C4692481 @default.
• W2146191961 hasConcept C49105822 @default.
• W2146191961 hasConcept C54355233 @default.
• W2146191961 hasConcept C54602769 @default.
• W2146191961 hasConcept C60987743 @default.
• W2146191961 hasConcept C86803240 @default.
• W2146191961 hasConceptScore W2146191961C104317684 @default.
• W2146191961 hasConceptScore W2146191961C105580179 @default.
• W2146191961 hasConceptScore W2146191961C141231307 @default.
• W2146191961 hasConceptScore W2146191961C156719811 @default.
• W2146191961 hasConceptScore W2146191961C159047783 @default.
• W2146191961 hasConceptScore W2146191961C2522874641 @default.
• W2146191961 hasConceptScore W2146191961C2777510210 @default.
• W2146191961 hasConceptScore W2146191961C2778271344 @default.
• W2146191961 hasConceptScore W2146191961C2778400998 @default.
• W2146191961 hasConceptScore W2146191961C2779005923 @default.
• W2146191961 hasConceptScore W2146191961C2780727368 @default.
• W2146191961 hasConceptScore W2146191961C4692481 @default.
• W2146191961 hasConceptScore W2146191961C49105822 @default.
• W2146191961 hasConceptScore W2146191961C54355233 @default.
• W2146191961 hasConceptScore W2146191961C54602769 @default.
• W2146191961 hasConceptScore W2146191961C60987743 @default.
• W2146191961 hasConceptScore W2146191961C86803240 @default.
• W2146191961 hasIssue "4" @default.
• W2146191961 hasLocation W21461919611 @default.
• W2146191961 hasLocation W21461919612 @default.
• W2146191961 hasOpenAccess W2146191961 @default.
• W2146191961 hasPrimaryLocation W21461919611 @default.
• W2146191961 hasRelatedWork W2032134460 @default.
• W2146191961 hasRelatedWork W2061951215 @default.
• W2146191961 hasRelatedWork W2094818532 @default.

• next