FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2146196287> ?p ?o ?g. }

• W2146196287 endingPage "564" @default.
• W2146196287 startingPage "548" @default.
• W2146196287 abstract "The first crystallographic structures of a catechol 1,2-dioxygenase from a Gram-positive bacterium Rhodococcus opacus 1CP (Rho 1,2-CTD), a Fe(III) ion containing enzyme specialized in the aerobic biodegradation of catechols, and its adducts with catechol, 3-methylcatechol, 4-methylcatechol, pyrogallol (benzene-1,2,3-triol), 3-chlorocatechol, 4-chlorocatechol, 3,5-dichlorocatechol, 4,5-dichlorocatechol and protocatechuate (3,4-dihydroxybenzoate) have been determined and analyzed. This study represents the first extensive characterization of catechols adducts of 1,2-CTDs. The structural analyses reveal the diverse modes of binding to the active metal iron ion of the tested catechols thus allowing to identify the residues selectively involved in recognition of the diverse substrates by this class of enzymes. The comparison is further extended to the structural and functional characteristics of the other 1,2-CTDs isolated from Gram-positive and Gram-negative bacteria. Moreover the high structural homology of the present enzyme with the 3-chlorocatechol 1,2-dioxygenase from the same bacterium are discussed in terms of their different substrate specificity. The catalytic rates for Rho 1,2-CTD conversion of the tested compounds are also compared with the calculated energies of the highest occupied molecular orbital (EHOMO) of the substrates. A quantitative relationship (R = 0.966) between the ln kcat and the calculated electronic parameter EHOMO was obtained for catechol, 3-methylcatechol, 4-methylcatechol, pyrogallol, 3-chlorocatechol, 4-chlorocatechol. This indicates that for these substrates the rate-limiting step of the reaction cycle is dependent on their nucleophilic reactivity. The discrepancies observed in the quantitative relationship for 3,5-dichlorocatechol, 4,5-dichlorocatechol and protocatechuate are ascribed to the sterical hindrances leading to the distorted binding of such catechols observed in the corresponding structures." @default.
• W2146196287 created "2016-06-24" @default.
• W2146196287 creator A5011855106 @default.
• W2146196287 creator A5012797490 @default.
• W2146196287 creator A5019184633 @default.
• W2146196287 creator A5031417252 @default.
• W2146196287 creator A5052700264 @default.
• W2146196287 creator A5075489987 @default.
• W2146196287 date "2010-06-01" @default.
• W2146196287 modified "2023-10-12" @default.
• W2146196287 title "Catechol 1,2-dioxygenase from the Gram-positive Rhodococcus opacus 1CP: Quantitative structure/activity relationship and the crystal structures of native enzyme and catechols adducts" @default.
• W2146196287 cites W1520730837 @default.
• W2146196287 cites W1553529773 @default.
• W2146196287 cites W1591191945 @default.
• W2146196287 cites W1822576021 @default.
• W2146196287 cites W1885778179 @default.
• W2146196287 cites W1965277349 @default.
• W2146196287 cites W1986191025 @default.
• W2146196287 cites W1990274831 @default.
• W2146196287 cites W1991354940 @default.
• W2146196287 cites W1994613702 @default.
• W2146196287 cites W2001641653 @default.
• W2146196287 cites W2002886549 @default.
• W2146196287 cites W2044179958 @default.
• W2146196287 cites W2048267273 @default.
• W2146196287 cites W2052895859 @default.
• W2146196287 cites W2055501774 @default.
• W2146196287 cites W2056081749 @default.
• W2146196287 cites W2058325166 @default.
• W2146196287 cites W2066596123 @default.
• W2146196287 cites W2069755108 @default.
• W2146196287 cites W2075271223 @default.
• W2146196287 cites W2076957858 @default.
• W2146196287 cites W2077973224 @default.
• W2146196287 cites W2091975638 @default.
• W2146196287 cites W2092670056 @default.
• W2146196287 cites W2100837269 @default.
• W2146196287 cites W2103235992 @default.
• W2146196287 cites W2105321472 @default.
• W2146196287 cites W2109499753 @default.
• W2146196287 cites W2118455767 @default.
• W2146196287 cites W2132629607 @default.
• W2146196287 cites W2136317897 @default.
• W2146196287 cites W2137777706 @default.
• W2146196287 cites W2139918194 @default.
• W2146196287 cites W2144081223 @default.
• W2146196287 cites W2169459092 @default.
• W2146196287 cites W2409645572 @default.
• W2146196287 cites W28461737 @default.
• W2146196287 cites W33750753 @default.
• W2146196287 cites W974572515 @default.
• W2146196287 doi "https://doi.org/10.1016/j.jsb.2009.12.023" @default.
• W2146196287 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20040374" @default.
• W2146196287 hasPublicationYear "2010" @default.
• W2146196287 type Work @default.
• W2146196287 sameAs 2146196287 @default.
• W2146196287 citedByCount "45" @default.
• W2146196287 countsByYear W21461962872012 @default.
• W2146196287 countsByYear W21461962872013 @default.
• W2146196287 countsByYear W21461962872014 @default.
• W2146196287 countsByYear W21461962872015 @default.
• W2146196287 countsByYear W21461962872016 @default.
• W2146196287 countsByYear W21461962872017 @default.
• W2146196287 countsByYear W21461962872018 @default.
• W2146196287 countsByYear W21461962872019 @default.
• W2146196287 countsByYear W21461962872020 @default.
• W2146196287 countsByYear W21461962872021 @default.
• W2146196287 countsByYear W21461962872022 @default.
• W2146196287 countsByYear W21461962872023 @default.
• W2146196287 crossrefType "journal-article" @default.
• W2146196287 hasAuthorship W2146196287A5011855106 @default.
• W2146196287 hasAuthorship W2146196287A5012797490 @default.
• W2146196287 hasAuthorship W2146196287A5019184633 @default.
• W2146196287 hasAuthorship W2146196287A5031417252 @default.
• W2146196287 hasAuthorship W2146196287A5052700264 @default.
• W2146196287 hasAuthorship W2146196287A5075489987 @default.
• W2146196287 hasConcept C108204754 @default.
• W2146196287 hasConcept C178790620 @default.
• W2146196287 hasConcept C181199279 @default.
• W2146196287 hasConcept C185592680 @default.
• W2146196287 hasConcept C2776344562 @default.
• W2146196287 hasConcept C2776834422 @default.
• W2146196287 hasConcept C2777550763 @default.
• W2146196287 hasConcept C2777713698 @default.
• W2146196287 hasConcept C71240020 @default.
• W2146196287 hasConceptScore W2146196287C108204754 @default.
• W2146196287 hasConceptScore W2146196287C178790620 @default.
• W2146196287 hasConceptScore W2146196287C181199279 @default.
• W2146196287 hasConceptScore W2146196287C185592680 @default.
• W2146196287 hasConceptScore W2146196287C2776344562 @default.
• W2146196287 hasConceptScore W2146196287C2776834422 @default.
• W2146196287 hasConceptScore W2146196287C2777550763 @default.
• W2146196287 hasConceptScore W2146196287C2777713698 @default.
• W2146196287 hasConceptScore W2146196287C71240020 @default.
• W2146196287 hasIssue "3" @default.
• W2146196287 hasLocation W21461962871 @default.
• W2146196287 hasLocation W21461962872 @default.
• W2146196287 hasOpenAccess W2146196287 @default.

• next