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• W2146200207 abstract "Most current guidelines recommend two serological tests to diagnose chronic Chagas disease. When serological tests are persistently inconclusive, some guidelines recommend molecular tests. The aim of this investigation was to review chronic Chagas disease diagnosis literature and to summarize results of ELISA and PCR performance.A systematic review was conducted searching remote databases (MEDLINE, LILACS, EMBASE, SCOPUS and ISIWeb) and full texts bibliography for relevant abstracts. In addition, manufacturers of commercial tests were contacted. Original investigations were eligible if they estimated sensitivity and specificity, or reliability -or if their calculation was possible - of ELISA or PCR tests, for chronic Chagas disease.Heterogeneity was high within each test (ELISA and PCR) and threshold effect was detected only in a particular subgroup. Reference standard blinding partially explained heterogeneity in ELISA studies, and pooled sensitivity and specificity were 97.7% [96.7%-98.5%] and 96.3% [94.6%-97.6%] respectively. Commercial ELISA with recombinant antigens studied in phase three investigations partially explained heterogeneity, and pooled sensitivity and specificity were 99.3% [97.9%-99.9%] and 97.5% [88.5%-99.5%] respectively. ELISA's reliability was seldom studied but was considered acceptable. PCR heterogeneity was not explained, but a threshold effect was detected in three groups created by using guanidine and boiling the sample before DNA extraction. PCR sensitivity is likely to be between 50% and 90%, while its specificity is close to 100%. PCR reliability was never studied.Both conventional and recombinant based ELISA give useful information, however there are commercial tests without technical reports and therefore were not included in this review. Physicians need to have access to technical reports to understand if these serological tests are similar to those included in this review and therefore correctly order and interpret test results. Currently, PCR should not be used in clinical practice for chronic Chagas disease diagnosis and there is no PCR test commercially available for this purpose. Tests limitations and directions for future research are discussed." @default.
• W2146200207 created "2016-06-24" @default.
• W2146200207 creator A5040242099 @default.
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• W2146200207 creator A5086636243 @default.
• W2146200207 date "2010-11-25" @default.
• W2146200207 modified "2023-10-17" @default.
• W2146200207 title "ELISA versus PCR for diagnosis of chronic Chagas disease: systematic review and meta-analysis" @default.
• W2146200207 cites W1211542888 @default.
• W2146200207 cites W1444727221 @default.
• W2146200207 cites W1754090969 @default.
• W2146200207 cites W1827172084 @default.
• W2146200207 cites W1881444952 @default.
• W2146200207 cites W1926152393 @default.
• W2146200207 cites W1964363252 @default.
• W2146200207 cites W1966208243 @default.
• W2146200207 cites W1970083218 @default.
• W2146200207 cites W1970692284 @default.
• W2146200207 cites W1974782847 @default.
• W2146200207 cites W1974828746 @default.
• W2146200207 cites W1978484035 @default.
• W2146200207 cites W1981322509 @default.
• W2146200207 cites W1982752710 @default.
• W2146200207 cites W1984837689 @default.
• W2146200207 cites W1988046751 @default.
• W2146200207 cites W1988601444 @default.
• W2146200207 cites W1991993040 @default.
• W2146200207 cites W1992670005 @default.
• W2146200207 cites W1993909120 @default.
• W2146200207 cites W1995792251 @default.
• W2146200207 cites W1996086920 @default.
• W2146200207 cites W1997188707 @default.
• W2146200207 cites W2000371172 @default.
• W2146200207 cites W2007880692 @default.
• W2146200207 cites W2008612295 @default.
• W2146200207 cites W2009260586 @default.
• W2146200207 cites W2013276329 @default.
• W2146200207 cites W2015647554 @default.
• W2146200207 cites W2018640745 @default.
• W2146200207 cites W2020264155 @default.
• W2146200207 cites W2021131747 @default.
• W2146200207 cites W2022499347 @default.
• W2146200207 cites W2023115639 @default.
• W2146200207 cites W2030666209 @default.
• W2146200207 cites W2035578264 @default.
• W2146200207 cites W2035788663 @default.
• W2146200207 cites W2036008244 @default.
• W2146200207 cites W2037244020 @default.
• W2146200207 cites W2039840943 @default.
• W2146200207 cites W2042958576 @default.
• W2146200207 cites W2045132016 @default.
• W2146200207 cites W2046460184 @default.
• W2146200207 cites W2050423980 @default.
• W2146200207 cites W2050814239 @default.
• W2146200207 cites W2052091890 @default.
• W2146200207 cites W2056698477 @default.
• W2146200207 cites W2057743943 @default.
• W2146200207 cites W2058484100 @default.
• W2146200207 cites W2060382330 @default.
• W2146200207 cites W2065002789 @default.
• W2146200207 cites W2065953288 @default.
• W2146200207 cites W2068201717 @default.
• W2146200207 cites W2068794323 @default.
• W2146200207 cites W2069057074 @default.
• W2146200207 cites W2069863089 @default.
• W2146200207 cites W2072682571 @default.
• W2146200207 cites W2077952389 @default.
• W2146200207 cites W2078109826 @default.
• W2146200207 cites W2084810230 @default.
• W2146200207 cites W2087734610 @default.
• W2146200207 cites W2089593775 @default.
• W2146200207 cites W2093394705 @default.
• W2146200207 cites W2094181685 @default.
• W2146200207 cites W2095166302 @default.
• W2146200207 cites W2099759474 @default.
• W2146200207 cites W2102748706 @default.
• W2146200207 cites W2102828205 @default.
• W2146200207 cites W2106485985 @default.
• W2146200207 cites W2107889929 @default.
• W2146200207 cites W2114865660 @default.
• W2146200207 cites W2118980852 @default.
• W2146200207 cites W2123565103 @default.
• W2146200207 cites W2124210282 @default.
• W2146200207 cites W2125214381 @default.
• W2146200207 cites W2125435699 @default.
• W2146200207 cites W2127013930 @default.
• W2146200207 cites W2132390016 @default.
• W2146200207 cites W2132737217 @default.
• W2146200207 cites W2134344666 @default.
• W2146200207 cites W2137821925 @default.
• W2146200207 cites W2140575048 @default.
• W2146200207 cites W2146976538 @default.
• W2146200207 cites W2147493587 @default.
• W2146200207 cites W2147560790 @default.
• W2146200207 cites W2147761124 @default.
• W2146200207 cites W2149258299 @default.
• W2146200207 cites W2151166219 @default.
• W2146200207 cites W2155883358 @default.

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