FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2146207911> ?p ?o ?g. }

• W2146207911 endingPage "702" @default.
• W2146207911 startingPage "695" @default.
• W2146207911 abstract "G protein-regulated inducer of neurite outgrowth 1 (GRIN1) was initially identified as a binding protein for guanosine 5′-3-<i>O</i>-(thio)triphosphate–bound Gαz. GRIN1 is specifically expressed in brain and interacts selectively with activated α subunits of the Gi subfamily. GRIN1 colocalizes with Gαo at the growth cone of neuronal cells and promotes neurite extension in Neuro2a cells when coexpressed with constitutively active mutant GαoQ205L. These results suggest that GRIN1 functions as a downstream target for Gαo. However, GRIN1 does not contain domains that are homologous to known signaling motifs. To understand the mechanisms of Gαo-GRIN1 pathway, we analyzed functional domains of GRIN1 that are involved in binding with Gαo or with its targeting to the plasma membrane. Using pull-down assays with glutathione <i>S</i>-transferase–fused GRIN1 deletion mutants, Gαo binding regions were localized to amino acid residues 716 to 746 and 797 to 827 of GRIN1. The Gαo binding region of GRIN1 did not demonstrate GTPase accelerating activity for Gαo. GRIN1 localized in the cell periphery in Neuro2a cells, and two cysteine residues at C-terminal region of GRIN1 (Cys818 and Cys819) were shown to be critical for its membrane targeting. Coexpression of GRIN1 with GαoQ205L or GRIN1Δ(717–827), which lacks Gαo binding region, promoted microspike formation in Swiss 3T3 cells or neurite extension in Neuro2a cells. The dominant-negative mutant of Cdc42 blocked these morphological changes. Coexpression of GRIN1 and GαoQ205L stimulated the formation of GTP-bound Cdc42 in Swiss 3T3 cells. These results suggest that the binding of activated Gαo to GRIN1 induces activation of Cdc42, which leads to morphological changes in neuronal cells." @default.
• W2146207911 created "2016-06-24" @default.
• W2146207911 creator A5010481814 @default.
• W2146207911 creator A5083797018 @default.
• W2146207911 date "2004-12-07" @default.
• W2146207911 modified "2023-09-30" @default.
• W2146207911 title "Functional Characterization of Gαo Signaling through G Protein-Regulated Inducer of Neurite Outgrowth 1" @default.
• W2146207911 cites W1488759788 @default.
• W2146207911 cites W1953109900 @default.
• W2146207911 cites W1967831804 @default.
• W2146207911 cites W1971707438 @default.
• W2146207911 cites W2001725097 @default.
• W2146207911 cites W2004581292 @default.
• W2146207911 cites W2015922146 @default.
• W2146207911 cites W2016221462 @default.
• W2146207911 cites W2019731805 @default.
• W2146207911 cites W2025444657 @default.
• W2146207911 cites W2029445497 @default.
• W2146207911 cites W2069629128 @default.
• W2146207911 cites W2074601908 @default.
• W2146207911 cites W2079865049 @default.
• W2146207911 cites W2080145523 @default.
• W2146207911 cites W2080875376 @default.
• W2146207911 cites W2106267269 @default.
• W2146207911 cites W2117918340 @default.
• W2146207911 cites W2127371010 @default.
• W2146207911 cites W2128806251 @default.
• W2146207911 cites W2130196728 @default.
• W2146207911 cites W2136921815 @default.
• W2146207911 cites W2139578251 @default.
• W2146207911 cites W2148310842 @default.
• W2146207911 cites W2154655439 @default.
• W2146207911 cites W4248350370 @default.
• W2146207911 doi "https://doi.org/10.1124/mol.104.003913" @default.
• W2146207911 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15585744" @default.
• W2146207911 hasPublicationYear "2004" @default.
• W2146207911 type Work @default.
• W2146207911 sameAs 2146207911 @default.
• W2146207911 citedByCount "38" @default.
• W2146207911 countsByYear W21462079112012 @default.
• W2146207911 countsByYear W21462079112013 @default.
• W2146207911 countsByYear W21462079112014 @default.
• W2146207911 countsByYear W21462079112015 @default.
• W2146207911 countsByYear W21462079112016 @default.
• W2146207911 countsByYear W21462079112017 @default.
• W2146207911 countsByYear W21462079112018 @default.
• W2146207911 countsByYear W21462079112019 @default.
• W2146207911 countsByYear W21462079112020 @default.
• W2146207911 countsByYear W21462079112021 @default.
• W2146207911 countsByYear W21462079112023 @default.
• W2146207911 crossrefType "journal-article" @default.
• W2146207911 hasAuthorship W2146207911A5010481814 @default.
• W2146207911 hasAuthorship W2146207911A5083797018 @default.
• W2146207911 hasConcept C104317684 @default.
• W2146207911 hasConcept C113246987 @default.
• W2146207911 hasConcept C143065580 @default.
• W2146207911 hasConcept C153911025 @default.
• W2146207911 hasConcept C185592680 @default.
• W2146207911 hasConcept C202751555 @default.
• W2146207911 hasConcept C55493867 @default.
• W2146207911 hasConcept C86803240 @default.
• W2146207911 hasConcept C95444343 @default.
• W2146207911 hasConceptScore W2146207911C104317684 @default.
• W2146207911 hasConceptScore W2146207911C113246987 @default.
• W2146207911 hasConceptScore W2146207911C143065580 @default.
• W2146207911 hasConceptScore W2146207911C153911025 @default.
• W2146207911 hasConceptScore W2146207911C185592680 @default.
• W2146207911 hasConceptScore W2146207911C202751555 @default.
• W2146207911 hasConceptScore W2146207911C55493867 @default.
• W2146207911 hasConceptScore W2146207911C86803240 @default.
• W2146207911 hasConceptScore W2146207911C95444343 @default.
• W2146207911 hasIssue "3" @default.
• W2146207911 hasLocation W21462079111 @default.
• W2146207911 hasLocation W21462079112 @default.
• W2146207911 hasOpenAccess W2146207911 @default.
• W2146207911 hasPrimaryLocation W21462079111 @default.
• W2146207911 hasRelatedWork W2038348068 @default.
• W2146207911 hasRelatedWork W2064207257 @default.
• W2146207911 hasRelatedWork W2150065613 @default.
• W2146207911 hasRelatedWork W2156423843 @default.
• W2146207911 hasRelatedWork W2156674132 @default.
• W2146207911 hasRelatedWork W2578524419 @default.
• W2146207911 hasRelatedWork W2973312646 @default.
• W2146207911 hasRelatedWork W3005078839 @default.
• W2146207911 hasRelatedWork W3044670305 @default.
• W2146207911 hasRelatedWork W4247436401 @default.
• W2146207911 hasVolume "67" @default.
• W2146207911 isParatext "false" @default.
• W2146207911 isRetracted "false" @default.
• W2146207911 magId "2146207911" @default.
• W2146207911 workType "article" @default.