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• W2146231030 endingPage "1697" @default.
• W2146231030 startingPage "1683" @default.
• W2146231030 abstract "Many plant phytochemicals constitute binary enzyme–glucoside systems and function in plant defence. In brassicas, the enzyme myrosinase is confined to specific myrosin cells that separate the enzyme from its substrate; the glucosinolates. The myrosinase-catalysed release of toxic and bioactive compounds such as isothiocyanates, upon activation or tissue damage, has been termed ‘the mustard oil bomb’ and characterized as a ‘toxic mine’ in plant defence. The removal of myrosin cells and the enzyme that triggers the release of phytochemicals have been investigated by genetically modifying Brassica napus plants to remove myrosinase-storing idioblasts. A construct with the seed myrosin cell-specific Myr1.Bn1 promoter was used to express a ribonuclease, barnase. Transgenic plants ectopically expressing barnase were embryo lethal. Co-expressing barnase under the control of the Myr1.Bn1 promoter with the barnase inhibitor, barstar, under the control of the cauliflower mosaic virus 35S promoter enabled a selective and controlled death of myrosin cells without affecting plant viability. Ablation of myrosin cells was confirmed with light and electron microscopy, with immunohistological analysis and immunogold-electron microscopy analysis showing empty holes where myrosin cells normally are localized. Further evidence for a successful myrosin cell ablation comes from immunoblots showing absence of myrosinase and negligible myrosinase activity, and autolysis experiments showing negligible production of glucosinolate hydrolysis products. The plants where the myrosin defence cells have been ablated and named ‘MINELESS plants’. The epithiospecifier protein profile and glucosinolate levels were changed in MINELESS plants, pointing to localization of myrosinases and a 35 kDa epithiospecifier protein in myrosin cells and a reduced turnover of glucosinolates in MINELESS plants." @default.
• W2146231030 created "2016-06-24" @default.
• W2146231030 creator A5023541878 @default.
• W2146231030 creator A5034019440 @default.
• W2146231030 creator A5049922665 @default.
• W2146231030 creator A5052199121 @default.
• W2146231030 creator A5078597030 @default.
• W2146231030 date "2010-03-10" @default.
• W2146231030 modified "2023-10-12" @default.
• W2146231030 title "Removing the mustard oil bomb from seeds: transgenic ablation of myrosin cells in oilseed rape (Brassica napus) produces MINELESS seeds" @default.
• W2146231030 cites W1494180378 @default.
• W2146231030 cites W1506500271 @default.
• W2146231030 cites W1563107533 @default.
• W2146231030 cites W1800449286 @default.
• W2146231030 cites W1885123225 @default.
• W2146231030 cites W1965859342 @default.
• W2146231030 cites W1967527546 @default.
• W2146231030 cites W1972857165 @default.
• W2146231030 cites W1976216831 @default.
• W2146231030 cites W1978207956 @default.
• W2146231030 cites W1979161283 @default.
• W2146231030 cites W1979330902 @default.
• W2146231030 cites W1982425197 @default.
• W2146231030 cites W1984050463 @default.
• W2146231030 cites W1986844963 @default.
• W2146231030 cites W1987370767 @default.
• W2146231030 cites W1987647818 @default.
• W2146231030 cites W1987715043 @default.
• W2146231030 cites W1988171164 @default.
• W2146231030 cites W1989258552 @default.
• W2146231030 cites W1992015062 @default.
• W2146231030 cites W1992528349 @default.
• W2146231030 cites W1992811877 @default.
• W2146231030 cites W1995111832 @default.
• W2146231030 cites W1995828353 @default.
• W2146231030 cites W1996688531 @default.
• W2146231030 cites W1997109001 @default.
• W2146231030 cites W1999797566 @default.
• W2146231030 cites W2000526965 @default.
• W2146231030 cites W2005927673 @default.
• W2146231030 cites W2006635962 @default.
• W2146231030 cites W2006889167 @default.
• W2146231030 cites W2008029873 @default.
• W2146231030 cites W2012747350 @default.
• W2146231030 cites W2017020973 @default.
• W2146231030 cites W2017073911 @default.
• W2146231030 cites W2018589046 @default.
• W2146231030 cites W2021427465 @default.
• W2146231030 cites W2024556021 @default.
• W2146231030 cites W2024684667 @default.
• W2146231030 cites W2027598034 @default.
• W2146231030 cites W2028980499 @default.
• W2146231030 cites W2029121247 @default.
• W2146231030 cites W2029841573 @default.
• W2146231030 cites W2038798476 @default.
• W2146231030 cites W2041550216 @default.
• W2146231030 cites W2044050143 @default.
• W2146231030 cites W2046517207 @default.
• W2146231030 cites W2047466209 @default.
• W2146231030 cites W2051825345 @default.
• W2146231030 cites W2054102617 @default.
• W2146231030 cites W2054839555 @default.
• W2146231030 cites W2056822423 @default.
• W2146231030 cites W2059667170 @default.
• W2146231030 cites W2065698231 @default.
• W2146231030 cites W2066096597 @default.
• W2146231030 cites W2066376507 @default.
• W2146231030 cites W2067100542 @default.
• W2146231030 cites W2067939166 @default.
• W2146231030 cites W2072496743 @default.
• W2146231030 cites W2072775452 @default.
• W2146231030 cites W2074781808 @default.
• W2146231030 cites W2075332154 @default.
• W2146231030 cites W2079916420 @default.
• W2146231030 cites W2080924397 @default.
• W2146231030 cites W2083178847 @default.
• W2146231030 cites W2085793386 @default.
• W2146231030 cites W2086545969 @default.
• W2146231030 cites W2089001046 @default.
• W2146231030 cites W2090031894 @default.
• W2146231030 cites W2093492034 @default.
• W2146231030 cites W2093744640 @default.
• W2146231030 cites W2099249565 @default.
• W2146231030 cites W2099299146 @default.
• W2146231030 cites W2100952501 @default.
• W2146231030 cites W2102554182 @default.
• W2146231030 cites W2106515175 @default.
• W2146231030 cites W2108735352 @default.
• W2146231030 cites W2114236143 @default.
• W2146231030 cites W2115037790 @default.
• W2146231030 cites W2117805596 @default.
• W2146231030 cites W2122098323 @default.
• W2146231030 cites W2122772580 @default.
• W2146231030 cites W2125864422 @default.
• W2146231030 cites W2126853906 @default.
• W2146231030 cites W2130208172 @default.
• W2146231030 cites W2142767735 @default.
• W2146231030 cites W2147351155 @default.

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