FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2146236564> ?p ?o ?g. }

• W2146236564 endingPage "1097" @default.
• W2146236564 startingPage "1088" @default.
• W2146236564 abstract "The crystal structures of the far-red fluorescent proteins (FPs) eqFP650 (λexmax/λemmax 592/650 nm) and eqFP670 (λexmax/λemmax 605/670 nm), the successors of the far-red FP Katushka (λexmax/λemmax 588/635 nm), have been determined at 1.8 and 1.6 Å resolution, respectively. An examination of the structures demonstrated that there are two groups of changes responsible for the bathochromic shift of excitation/emission bands of these proteins relative to their predecessor. The first group of changes resulted in an increase of hydrophilicity at the acylimine site of the chromophore due to the presence of one and three water molecules in eqFP650 and eqFP670, respectively. These water molecules provide connection of the chromophore with the protein scaffold via hydrogen bonds causing an ∼15 nm bathochromic shift of the eqFP650 and eqFP670 emission bands. The second group of changes observed in eqFP670 arises from substitution of both Ser143 and Ser158 by asparagines. Asn143 and Asn158 of eqFP670 are hydrogen bonded with each other, as well as with the protein scaffold and with the p-hydroxyphenyl group of the chromophore, resulting in an additional ∼20 nm bathochromic shift of the eqFP670 emission band as compared to eqFP650. The role of the observed structural changes was verified by mutagenesis." @default.
• W2146236564 created "2016-06-24" @default.
• W2146236564 creator A5015434460 @default.
• W2146236564 creator A5016035860 @default.
• W2146236564 creator A5019975859 @default.
• W2146236564 creator A5027980832 @default.
• W2146236564 creator A5031117472 @default.
• W2146236564 creator A5047004561 @default.
• W2146236564 creator A5056346975 @default.
• W2146236564 creator A5060028352 @default.
• W2146236564 date "2012-08-18" @default.
• W2146236564 modified "2023-10-16" @default.
• W2146236564 title "Structural basis for bathochromic shift of fluorescence in far-red fluorescent proteins eqFP650 and eqFP670" @default.
• W2146236564 cites W1522693579 @default.
• W2146236564 cites W1539796472 @default.
• W2146236564 cites W1972651124 @default.
• W2146236564 cites W1986191025 @default.
• W2146236564 cites W1992875306 @default.
• W2146236564 cites W2022871424 @default.
• W2146236564 cites W2038756774 @default.
• W2146236564 cites W2038840577 @default.
• W2146236564 cites W2040539483 @default.
• W2146236564 cites W2045346173 @default.
• W2146236564 cites W2047303494 @default.
• W2146236564 cites W2052512546 @default.
• W2146236564 cites W2057397408 @default.
• W2146236564 cites W2058211348 @default.
• W2146236564 cites W2067492027 @default.
• W2146236564 cites W2073046174 @default.
• W2146236564 cites W2078366572 @default.
• W2146236564 cites W2091522551 @default.
• W2146236564 cites W2097493124 @default.
• W2146236564 cites W2105332737 @default.
• W2146236564 cites W2107913200 @default.
• W2146236564 cites W2109184569 @default.
• W2146236564 cites W2112947216 @default.
• W2146236564 cites W2122683100 @default.
• W2146236564 cites W2122881260 @default.
• W2146236564 cites W2128583282 @default.
• W2146236564 cites W2133061035 @default.
• W2146236564 cites W2134190459 @default.
• W2146236564 cites W2136133782 @default.
• W2146236564 cites W2144081223 @default.
• W2146236564 cites W2163191416 @default.
• W2146236564 cites W2163474990 @default.
• W2146236564 cites W2170682202 @default.
• W2146236564 doi "https://doi.org/10.1107/s0907444912020598" @default.
• W2146236564 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3489099" @default.
• W2146236564 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22948909" @default.
• W2146236564 hasPublicationYear "2012" @default.
• W2146236564 type Work @default.
• W2146236564 sameAs 2146236564 @default.
• W2146236564 citedByCount "19" @default.
• W2146236564 countsByYear W21462365642013 @default.
• W2146236564 countsByYear W21462365642014 @default.
• W2146236564 countsByYear W21462365642015 @default.
• W2146236564 countsByYear W21462365642016 @default.
• W2146236564 countsByYear W21462365642017 @default.
• W2146236564 countsByYear W21462365642018 @default.
• W2146236564 countsByYear W21462365642019 @default.
• W2146236564 countsByYear W21462365642020 @default.
• W2146236564 countsByYear W21462365642021 @default.
• W2146236564 countsByYear W21462365642022 @default.
• W2146236564 countsByYear W21462365642023 @default.
• W2146236564 crossrefType "journal-article" @default.
• W2146236564 hasAuthorship W2146236564A5015434460 @default.
• W2146236564 hasAuthorship W2146236564A5016035860 @default.
• W2146236564 hasAuthorship W2146236564A5019975859 @default.
• W2146236564 hasAuthorship W2146236564A5027980832 @default.
• W2146236564 hasAuthorship W2146236564A5031117472 @default.
• W2146236564 hasAuthorship W2146236564A5047004561 @default.
• W2146236564 hasAuthorship W2146236564A5056346975 @default.
• W2146236564 hasAuthorship W2146236564A5060028352 @default.
• W2146236564 hasBestOaLocation W21462365642 @default.
• W2146236564 hasConcept C112887158 @default.
• W2146236564 hasConcept C120665830 @default.
• W2146236564 hasConcept C121332964 @default.
• W2146236564 hasConcept C178790620 @default.
• W2146236564 hasConcept C185592680 @default.
• W2146236564 hasConcept C192468462 @default.
• W2146236564 hasConcept C32909587 @default.
• W2146236564 hasConcept C46051903 @default.
• W2146236564 hasConcept C75473681 @default.
• W2146236564 hasConcept C8010536 @default.
• W2146236564 hasConcept C91881484 @default.
• W2146236564 hasConceptScore W2146236564C112887158 @default.
• W2146236564 hasConceptScore W2146236564C120665830 @default.
• W2146236564 hasConceptScore W2146236564C121332964 @default.
• W2146236564 hasConceptScore W2146236564C178790620 @default.
• W2146236564 hasConceptScore W2146236564C185592680 @default.
• W2146236564 hasConceptScore W2146236564C192468462 @default.
• W2146236564 hasConceptScore W2146236564C32909587 @default.
• W2146236564 hasConceptScore W2146236564C46051903 @default.
• W2146236564 hasConceptScore W2146236564C75473681 @default.
• W2146236564 hasConceptScore W2146236564C8010536 @default.
• W2146236564 hasConceptScore W2146236564C91881484 @default.
• W2146236564 hasIssue "9" @default.
• W2146236564 hasLocation W21462365641 @default.

• next