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• W2146276688 startingPage "841" @default.
• W2146276688 abstract "A sensitive staining method for protein blots using Direct Blue 71 is described. It is based on the selective binding of dye molecules to proteins in acidic solution and produces bluish violet colored bands. It is a simple and rapid procedure, involving only staining and rinsing steps that occur within 7 min. The sensitivity of this method is 5—10 ng of protein on nitrocellulose (NC) and 10—20 ng on polyvinylidene difluoride (PVDF), which is tenfold better than that of the commonly used Ponceau S staining. Moreover, the staining is reversible for subsequent immunostaining, without impairing immunoreactivity. To remove the dye from the developed bands, changes in pH and hydrophobicity of the solvent are required. Due to its sensitivity, rapidity, simplicity, and low cost, this stain may be more practical than other dye-based stains or metal-based stains for routine laboratory purposes." @default.
• W2146276688 created "2016-06-24" @default.
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• W2146276688 date "2000-03-01" @default.
• W2146276688 modified "2023-10-17" @default.
• W2146276688 title "Direct Blue 71 staining of proteins bound to blotting membranes" @default.
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• W2146276688 doi "https://doi.org/10.1002/(sici)1522-2683(20000301)21:5<841::aid-elps841>3.0.co;2-4" @default.
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