Matches in SemOpenAlex for { <https://semopenalex.org/work/W2146290432> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2146290432 endingPage "181" @default.
- W2146290432 startingPage "175" @default.
- W2146290432 abstract "We have developed a new subcloning-less method for generating nested deletions which we have termed Solid-Phase Nested Deletion. The basic procedure for this method is as follows. The target DNA fragment is cloned in the multiple cloning site of a cloning vector, pUC or its derivatives, and amplified by PCR using a set of primers, one of which is 5'-biotinylated. The amplified DNA is partially digested by a restriction enzyme with a 4-base recognition sequence. The digested DNA is ligated with a synthetic adapter DNA. Monodiverse beads coupled with streptavidin (Dynabeads M-280 streptavidin) are added to the mixture and the biotinylated DNA fragments are separated by applying magnetic field. The unidirectionally deleted DNA fragments are recovered by PCR from the magnetic beads, and size-fractionated by agarose gel electrophoresis. The DNA fragments are amplified by PCR and used for sequencing. We demonstrate the potential of this method using a 4878-bp EcoRI fragment of lambda phage DNA." @default.
- W2146290432 created "2016-06-24" @default.
- W2146290432 creator A5032695946 @default.
- W2146290432 creator A5043460153 @default.
- W2146290432 creator A5089339522 @default.
- W2146290432 date "1995-01-01" @default.
- W2146290432 modified "2023-09-23" @default.
- W2146290432 title "Solid-Phase Nested Deletion: A New Subcloning-less Method for Generating Nested Deletions" @default.
- W2146290432 cites W1979186179 @default.
- W2146290432 cites W1987666378 @default.
- W2146290432 cites W2029343098 @default.
- W2146290432 cites W2032462421 @default.
- W2146290432 cites W2090791734 @default.
- W2146290432 cites W2131626832 @default.
- W2146290432 cites W2416863564 @default.
- W2146290432 doi "https://doi.org/10.1093/dnares/2.4.175" @default.
- W2146290432 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8590281" @default.
- W2146290432 hasPublicationYear "1995" @default.
- W2146290432 type Work @default.
- W2146290432 sameAs 2146290432 @default.
- W2146290432 citedByCount "2" @default.
- W2146290432 crossrefType "journal-article" @default.
- W2146290432 hasAuthorship W2146290432A5032695946 @default.
- W2146290432 hasAuthorship W2146290432A5043460153 @default.
- W2146290432 hasAuthorship W2146290432A5089339522 @default.
- W2146290432 hasBestOaLocation W21462904321 @default.
- W2146290432 hasConcept C104317684 @default.
- W2146290432 hasConcept C119599485 @default.
- W2146290432 hasConcept C127413603 @default.
- W2146290432 hasConcept C128319531 @default.
- W2146290432 hasConcept C130123407 @default.
- W2146290432 hasConcept C153911025 @default.
- W2146290432 hasConcept C157431215 @default.
- W2146290432 hasConcept C163005120 @default.
- W2146290432 hasConcept C168374021 @default.
- W2146290432 hasConcept C177284502 @default.
- W2146290432 hasConcept C187882448 @default.
- W2146290432 hasConcept C19924922 @default.
- W2146290432 hasConcept C204628709 @default.
- W2146290432 hasConcept C22744801 @default.
- W2146290432 hasConcept C2776190903 @default.
- W2146290432 hasConcept C2778204606 @default.
- W2146290432 hasConcept C49105822 @default.
- W2146290432 hasConcept C54355233 @default.
- W2146290432 hasConcept C552990157 @default.
- W2146290432 hasConcept C86803240 @default.
- W2146290432 hasConceptScore W2146290432C104317684 @default.
- W2146290432 hasConceptScore W2146290432C119599485 @default.
- W2146290432 hasConceptScore W2146290432C127413603 @default.
- W2146290432 hasConceptScore W2146290432C128319531 @default.
- W2146290432 hasConceptScore W2146290432C130123407 @default.
- W2146290432 hasConceptScore W2146290432C153911025 @default.
- W2146290432 hasConceptScore W2146290432C157431215 @default.
- W2146290432 hasConceptScore W2146290432C163005120 @default.
- W2146290432 hasConceptScore W2146290432C168374021 @default.
- W2146290432 hasConceptScore W2146290432C177284502 @default.
- W2146290432 hasConceptScore W2146290432C187882448 @default.
- W2146290432 hasConceptScore W2146290432C19924922 @default.
- W2146290432 hasConceptScore W2146290432C204628709 @default.
- W2146290432 hasConceptScore W2146290432C22744801 @default.
- W2146290432 hasConceptScore W2146290432C2776190903 @default.
- W2146290432 hasConceptScore W2146290432C2778204606 @default.
- W2146290432 hasConceptScore W2146290432C49105822 @default.
- W2146290432 hasConceptScore W2146290432C54355233 @default.
- W2146290432 hasConceptScore W2146290432C552990157 @default.
- W2146290432 hasConceptScore W2146290432C86803240 @default.
- W2146290432 hasIssue "4" @default.
- W2146290432 hasLocation W21462904321 @default.
- W2146290432 hasLocation W21462904322 @default.
- W2146290432 hasOpenAccess W2146290432 @default.
- W2146290432 hasPrimaryLocation W21462904321 @default.
- W2146290432 hasRelatedWork W1497647308 @default.
- W2146290432 hasRelatedWork W1986510350 @default.
- W2146290432 hasRelatedWork W1987929054 @default.
- W2146290432 hasRelatedWork W1988049223 @default.
- W2146290432 hasRelatedWork W2003283696 @default.
- W2146290432 hasRelatedWork W2030691267 @default.
- W2146290432 hasRelatedWork W2032116141 @default.
- W2146290432 hasRelatedWork W2082400949 @default.
- W2146290432 hasRelatedWork W2146290432 @default.
- W2146290432 hasRelatedWork W2967994394 @default.
- W2146290432 hasVolume "2" @default.
- W2146290432 isParatext "false" @default.
- W2146290432 isRetracted "false" @default.
- W2146290432 magId "2146290432" @default.
- W2146290432 workType "article" @default.