Matches in SemOpenAlex for { <https://semopenalex.org/work/W2146307538> ?p ?o ?g. }
- W2146307538 endingPage "2726" @default.
- W2146307538 startingPage "2710" @default.
- W2146307538 abstract "Increased expression of the astrocytic intermediate filament protein glial fibrillary acidic protein (GFAP) is a characteristic of astrogliosis. This process occurs in the brain during aging and neurodegeneration and coincides with impairment of the ubiquitin proteasome system. Inhibition of the proteasome impairs protein degradation; therefore, we hypothesized that the increase in GFAP may be the result of impaired proteasomal activity in astrocytes. We investigated the effect of proteasome inhibitors on GFAP expression and other intermediate filament proteins in human astrocytoma cells and in a rat brain model for astrogliosis. Extensive quantitative RT-PCR, immunocytochemistry, and Western blot analysis resulted unexpectedly in a strong decrease of GFAP mRNA to <4% of control levels [Control (DMSO) 100+/-19.2%; proteasome inhibitor (epoxomicin) 3.5+/-1.3%, n=8; P < or = 0.001] and a loss of GFAP protein in astrocytes in vitro. We show that the proteasome alters GFAP promoter activity, possibly mediated by transcription factors as demonstrated by a GFAP promoter-luciferase assay and RT(2) Profiler PCR array for human transcription factors. Most important, we demonstrate that proteasome inhibitors also reduce GFAP and vimentin expression in a rat model for induced astrogliosis in vivo. Therefore, proteasome inhibitors could serve as a potential therapy to modulate astrogliosis associated with CNS injuries and disease." @default.
- W2146307538 created "2016-06-24" @default.
- W2146307538 creator A5006497097 @default.
- W2146307538 creator A5013180706 @default.
- W2146307538 creator A5015383958 @default.
- W2146307538 creator A5017096014 @default.
- W2146307538 creator A5048299435 @default.
- W2146307538 creator A5062435332 @default.
- W2146307538 creator A5067496439 @default.
- W2146307538 creator A5067856710 @default.
- W2146307538 creator A5087754740 @default.
- W2146307538 creator A5088622927 @default.
- W2146307538 creator A5089200576 @default.
- W2146307538 creator A5090136803 @default.
- W2146307538 date "2009-03-30" @default.
- W2146307538 modified "2023-10-02" @default.
- W2146307538 title "Intermediate filament transcription in astrocytes is repressed by proteasome inhibition" @default.
- W2146307538 cites W1484832749 @default.
- W2146307538 cites W1552427867 @default.
- W2146307538 cites W1570610869 @default.
- W2146307538 cites W1574133290 @default.
- W2146307538 cites W1586815960 @default.
- W2146307538 cites W1943067311 @default.
- W2146307538 cites W1966266513 @default.
- W2146307538 cites W1969995127 @default.
- W2146307538 cites W1970862254 @default.
- W2146307538 cites W1972436933 @default.
- W2146307538 cites W1973963820 @default.
- W2146307538 cites W1974012392 @default.
- W2146307538 cites W1974063341 @default.
- W2146307538 cites W1977380334 @default.
- W2146307538 cites W1977884779 @default.
- W2146307538 cites W1978669073 @default.
- W2146307538 cites W1978836458 @default.
- W2146307538 cites W1979228875 @default.
- W2146307538 cites W1979498042 @default.
- W2146307538 cites W1981502202 @default.
- W2146307538 cites W1981547140 @default.
- W2146307538 cites W1984009511 @default.
- W2146307538 cites W1986413832 @default.
- W2146307538 cites W1988074354 @default.
- W2146307538 cites W1989602074 @default.
- W2146307538 cites W1990594693 @default.
- W2146307538 cites W1992741271 @default.
- W2146307538 cites W1997627588 @default.
- W2146307538 cites W1999495459 @default.
- W2146307538 cites W2004716554 @default.
- W2146307538 cites W2009962604 @default.
- W2146307538 cites W2010476270 @default.
- W2146307538 cites W2015888105 @default.
- W2146307538 cites W2017946350 @default.
- W2146307538 cites W2019610319 @default.
- W2146307538 cites W2020999109 @default.
- W2146307538 cites W2021242983 @default.
- W2146307538 cites W2023754965 @default.
- W2146307538 cites W2026115693 @default.
- W2146307538 cites W2027119303 @default.
- W2146307538 cites W2027953381 @default.
- W2146307538 cites W2029738684 @default.
- W2146307538 cites W2031017877 @default.
- W2146307538 cites W2032389085 @default.
- W2146307538 cites W2034447471 @default.
- W2146307538 cites W2038834815 @default.
- W2146307538 cites W2038852478 @default.
- W2146307538 cites W2044130121 @default.
- W2146307538 cites W2045492362 @default.
- W2146307538 cites W2046314862 @default.
- W2146307538 cites W2048599247 @default.
- W2146307538 cites W2050634340 @default.
- W2146307538 cites W2052032313 @default.
- W2146307538 cites W2052694792 @default.
- W2146307538 cites W2056268708 @default.
- W2146307538 cites W2058277646 @default.
- W2146307538 cites W2058830580 @default.
- W2146307538 cites W2059930697 @default.
- W2146307538 cites W2060987736 @default.
- W2146307538 cites W2063377136 @default.
- W2146307538 cites W2063498603 @default.
- W2146307538 cites W2068586472 @default.
- W2146307538 cites W2069558973 @default.
- W2146307538 cites W2070530394 @default.
- W2146307538 cites W2071191421 @default.
- W2146307538 cites W2073004429 @default.
- W2146307538 cites W2076389168 @default.
- W2146307538 cites W2078428933 @default.
- W2146307538 cites W2078877797 @default.
- W2146307538 cites W2086324579 @default.
- W2146307538 cites W2087697089 @default.
- W2146307538 cites W2088496405 @default.
- W2146307538 cites W2096207943 @default.
- W2146307538 cites W2101335451 @default.
- W2146307538 cites W2103037576 @default.
- W2146307538 cites W2105729193 @default.
- W2146307538 cites W2105796226 @default.
- W2146307538 cites W2106142655 @default.
- W2146307538 cites W2120412594 @default.
- W2146307538 cites W2121101995 @default.
- W2146307538 cites W2121860798 @default.