Matches in SemOpenAlex for { <https://semopenalex.org/work/W2146370523> ?p ?o ?g. }
- W2146370523 endingPage "9456" @default.
- W2146370523 startingPage "9451" @default.
- W2146370523 abstract "The spread of multidrug-resistant Staphylococcus aureus (MRSA) strains in the clinical environment has begun to pose serious limits to treatment options. Yet virtually nothing is known about how resistance traits are acquired in vivo . Here, we apply the power of whole-genome sequencing to identify steps in the evolution of multidrug resistance in isogenic S. aureus isolates recovered periodically from the bloodstream of a patient undergoing chemotherapy with vancomycin and other antibiotics. After extensive therapy, the bacterium developed resistance, and treatment failed. Sequencing the first vancomycin susceptible isolate and the last vancomycin nonsusceptible isolate identified genome wide only 35 point mutations in 31 loci. These mutations appeared in a sequential order in isolates that were recovered at intermittent times during chemotherapy in parallel with increasing levels of resistance. The vancomycin nonsusceptible isolates also showed a 100-fold decrease in susceptibility to daptomycin, although this antibiotic was not used in the therapy. One of the mutated loci associated with decreasing vancomycin susceptibility (the vraR operon) was found to also carry mutations in six additional vancomycin nonsusceptible S. aureus isolates belonging to different genetic backgrounds and recovered from different geographic sites. As costs drop, whole-genome sequencing will become a useful tool in elucidating complex pathways of in vivo evolution in bacterial pathogens." @default.
- W2146370523 created "2016-06-24" @default.
- W2146370523 creator A5014218977 @default.
- W2146370523 creator A5018901369 @default.
- W2146370523 creator A5025937223 @default.
- W2146370523 creator A5036408112 @default.
- W2146370523 creator A5038568562 @default.
- W2146370523 creator A5066668322 @default.
- W2146370523 creator A5066701891 @default.
- W2146370523 creator A5068657716 @default.
- W2146370523 creator A5072115247 @default.
- W2146370523 creator A5076223619 @default.
- W2146370523 creator A5081757054 @default.
- W2146370523 date "2007-05-29" @default.
- W2146370523 modified "2023-10-14" @default.
- W2146370523 title "Tracking the <i>in vivo</i> evolution of multidrug resistance in <i>Staphylococcus aureus</i> by whole-genome sequencing" @default.
- W2146370523 cites W103897704 @default.
- W2146370523 cites W1761182137 @default.
- W2146370523 cites W1908139426 @default.
- W2146370523 cites W1967259298 @default.
- W2146370523 cites W1989797392 @default.
- W2146370523 cites W1991169313 @default.
- W2146370523 cites W1991179149 @default.
- W2146370523 cites W1994727418 @default.
- W2146370523 cites W2005401924 @default.
- W2146370523 cites W2034747690 @default.
- W2146370523 cites W2041975350 @default.
- W2146370523 cites W2051049929 @default.
- W2146370523 cites W2054160327 @default.
- W2146370523 cites W2066200885 @default.
- W2146370523 cites W2084448398 @default.
- W2146370523 cites W2097509506 @default.
- W2146370523 cites W2101134401 @default.
- W2146370523 cites W2108643808 @default.
- W2146370523 cites W2111993374 @default.
- W2146370523 cites W2118094416 @default.
- W2146370523 cites W2121952186 @default.
- W2146370523 cites W2126734480 @default.
- W2146370523 cites W2127747618 @default.
- W2146370523 cites W2129062666 @default.
- W2146370523 cites W2129543141 @default.
- W2146370523 cites W2135682495 @default.
- W2146370523 cites W2139677943 @default.
- W2146370523 cites W2141046353 @default.
- W2146370523 cites W2141837962 @default.
- W2146370523 cites W2144413761 @default.
- W2146370523 cites W2146894318 @default.
- W2146370523 cites W2147793137 @default.
- W2146370523 cites W2148663866 @default.
- W2146370523 cites W2150927648 @default.
- W2146370523 cites W2151693231 @default.
- W2146370523 cites W2153590024 @default.
- W2146370523 cites W2155167184 @default.
- W2146370523 cites W2157018267 @default.
- W2146370523 cites W2160244430 @default.
- W2146370523 cites W2161587331 @default.
- W2146370523 cites W2162139256 @default.
- W2146370523 cites W2166160777 @default.
- W2146370523 cites W2167665502 @default.
- W2146370523 cites W2168911650 @default.
- W2146370523 cites W2170367123 @default.
- W2146370523 cites W2336324580 @default.
- W2146370523 cites W4230708463 @default.
- W2146370523 cites W4242673342 @default.
- W2146370523 doi "https://doi.org/10.1073/pnas.0609839104" @default.
- W2146370523 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1890515" @default.
- W2146370523 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17517606" @default.
- W2146370523 hasPublicationYear "2007" @default.
- W2146370523 type Work @default.
- W2146370523 sameAs 2146370523 @default.
- W2146370523 citedByCount "490" @default.
- W2146370523 countsByYear W21463705232012 @default.
- W2146370523 countsByYear W21463705232013 @default.
- W2146370523 countsByYear W21463705232014 @default.
- W2146370523 countsByYear W21463705232015 @default.
- W2146370523 countsByYear W21463705232016 @default.
- W2146370523 countsByYear W21463705232017 @default.
- W2146370523 countsByYear W21463705232018 @default.
- W2146370523 countsByYear W21463705232019 @default.
- W2146370523 countsByYear W21463705232020 @default.
- W2146370523 countsByYear W21463705232021 @default.
- W2146370523 countsByYear W21463705232022 @default.
- W2146370523 countsByYear W21463705232023 @default.
- W2146370523 crossrefType "journal-article" @default.
- W2146370523 hasAuthorship W2146370523A5014218977 @default.
- W2146370523 hasAuthorship W2146370523A5018901369 @default.
- W2146370523 hasAuthorship W2146370523A5025937223 @default.
- W2146370523 hasAuthorship W2146370523A5036408112 @default.
- W2146370523 hasAuthorship W2146370523A5038568562 @default.
- W2146370523 hasAuthorship W2146370523A5066668322 @default.
- W2146370523 hasAuthorship W2146370523A5066701891 @default.
- W2146370523 hasAuthorship W2146370523A5068657716 @default.
- W2146370523 hasAuthorship W2146370523A5072115247 @default.
- W2146370523 hasAuthorship W2146370523A5076223619 @default.
- W2146370523 hasAuthorship W2146370523A5081757054 @default.
- W2146370523 hasBestOaLocation W21463705232 @default.
- W2146370523 hasConcept C104317684 @default.
- W2146370523 hasConcept C114851261 @default.