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• W2146393404 abstract "Microarrays have been applied to the determination of genome-wide expression patterns during the cell cycle of a number of different cells. Both eukaryotic and prokaryotic cells have been studied using whole-culture and selective synchronization methods. The published microarray data on yeast, mammalian, and bacterial cells have been uniformly interpreted as indicating that a large number of genes are expressed in a cell-cycle-dependent manner. These conclusions are reconsidered using explicit criteria for synchronization and precise criteria for identifying gene expression patterns during the cell cycle. The conclusions regarding cell-cycle-dependent gene expression based on microarray analysis are weakened by arguably problematic choices for synchronization methodology (e.g., whole-culture methods that do not synchronize cells) and questionable statistical rigor for identifying cell-cycle-dependent gene expression. Because of the uncertainties in synchrony methodology, as well as uncertainties in microarray analysis, one should be somewhat skeptical of claims that there are a large number of genes expressed in a cell-cycle-dependent manner." @default.
• W2146393404 created "2016-06-24" @default.
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• W2146393404 date "2003-01-01" @default.
• W2146393404 modified "2023-09-26" @default.
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• W2146393404 doi "https://doi.org/10.1186/1475-9268-2-1" @default.
• W2146393404 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/239863" @default.
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