FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2146408886> ?p ?o ?g. }

• W2146408886 endingPage "585" @default.
• W2146408886 startingPage "575" @default.
• W2146408886 abstract "Recently, we reported the human 88-kDa calcium-independent phospholipase A2 (iPLA2) cDNA sequence, as well as extensive alternative splicing of the iPLA2 mRNA. In this report we identified the gene coding for iPLA2, which was localized on chromosome 22q13.1. The gene consists of at least 17 exons spanning > 69 kb. Based on the iPLA2 gene organization the splice variants can be explained. The putative promotor for the iPLA2 gene lacks a TATA-box and contains a CpG island as well as several potential Sp-1-binding sites. Furthermore, the 5′-flanking region also contains one medium reiteration frequency repeat (MER53) and an Alu repetitive sequence. Northern blot analysis of iPLA2 mRNA in various human tissues demonstrated tissue-specific expression of four distinct iPLA2 transcripts. The native human 3.2-kb iPLA2 transcript was predominantly expressed in heart, brain, skeletal muscle, prostate, testis, thyroid and spinal cord, and to a lesser extent in peripheral blood leucocytes, stomach, trachea and bone marrow. Studies on the subcellular localization of the native iPLA2 protein were performed in COS-7 cells overexpressing this enzyme. The cytosolic fraction of untransfected and cells overexpressing iPLA2 contained equal amounts of calcium-independent PLA2 activity. However, the membrane fraction displayed a 5.5-fold increased activity in iPLA2 overexpressing cells. This increased calcium-independent PLA2 activity correlated with the presence of iPLA2 immunoreactive protein in the membrane fraction, indicating that this form of iPLA2 protein was membrane associated. Studies of iPLA2 in rat vascular smooth muscle cells verified the membrane association of this form of iPLA2. The major difference between this form of iPLA2 enzyme and the soluble forms of iPLA2 studied previously is the presence of 54 additional amino acid residues derived from exon 9. We suggest that the addition of these 54 amino acids leads to a membrane-associated protein. In summary, these results demonstrate that alternative splicing of the human iPLA2 transcript generates multiple iPLA2 isoforms with distinct tissue distribution and cellular localization." @default.
• W2146408886 created "2016-06-24" @default.
• W2146408886 creator A5000656223 @default.
• W2146408886 creator A5028530566 @default.
• W2146408886 creator A5036028850 @default.
• W2146408886 date "1999-06-01" @default.
• W2146408886 modified "2023-10-17" @default.
• W2146408886 title "The human calcium-independent phospholipase A2 gene . Multiple enzymes with distinct properties from a single gene" @default.
• W2146408886 cites W1490672339 @default.
• W2146408886 cites W1538690208 @default.
• W2146408886 cites W1557423604 @default.
• W2146408886 cites W1562176487 @default.
• W2146408886 cites W1573258040 @default.
• W2146408886 cites W1842434515 @default.
• W2146408886 cites W1957006795 @default.
• W2146408886 cites W1959359615 @default.
• W2146408886 cites W1969301447 @default.
• W2146408886 cites W1971421846 @default.
• W2146408886 cites W1975304761 @default.
• W2146408886 cites W1984487835 @default.
• W2146408886 cites W1992021138 @default.
• W2146408886 cites W1994395273 @default.
• W2146408886 cites W1996824520 @default.
• W2146408886 cites W2011661565 @default.
• W2146408886 cites W201873278 @default.
• W2146408886 cites W2022202114 @default.
• W2146408886 cites W2027188394 @default.
• W2146408886 cites W2027294838 @default.
• W2146408886 cites W2030093162 @default.
• W2146408886 cites W2030997003 @default.
• W2146408886 cites W2038177206 @default.
• W2146408886 cites W2040827725 @default.
• W2146408886 cites W2043380617 @default.
• W2146408886 cites W2047379678 @default.
• W2146408886 cites W2051265887 @default.
• W2146408886 cites W2051958752 @default.
• W2146408886 cites W2053869318 @default.
• W2146408886 cites W2055095365 @default.
• W2146408886 cites W2069404833 @default.
• W2146408886 cites W2074943389 @default.
• W2146408886 cites W2080664733 @default.
• W2146408886 cites W2082190759 @default.
• W2146408886 cites W2083611460 @default.
• W2146408886 cites W2084468357 @default.
• W2146408886 cites W2084748297 @default.
• W2146408886 cites W2090178694 @default.
• W2146408886 cites W2091783910 @default.
• W2146408886 cites W2101108802 @default.
• W2146408886 cites W2122167768 @default.
• W2146408886 cites W2141115187 @default.
• W2146408886 cites W2152713567 @default.
• W2146408886 cites W2163176016 @default.
• W2146408886 cites W2172202413 @default.
• W2146408886 cites W2407631651 @default.
• W2146408886 cites W2414020669 @default.
• W2146408886 cites W4211175188 @default.
• W2146408886 cites W4231811546 @default.
• W2146408886 cites W4243242947 @default.
• W2146408886 doi "https://doi.org/10.1046/j.1432-1327.1999.00418.x" @default.
• W2146408886 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10336645" @default.
• W2146408886 hasPublicationYear "1999" @default.
• W2146408886 type Work @default.
• W2146408886 sameAs 2146408886 @default.
• W2146408886 citedByCount "124" @default.
• W2146408886 countsByYear W21464088862012 @default.
• W2146408886 countsByYear W21464088862013 @default.
• W2146408886 countsByYear W21464088862014 @default.
• W2146408886 countsByYear W21464088862015 @default.
• W2146408886 countsByYear W21464088862016 @default.
• W2146408886 countsByYear W21464088862018 @default.
• W2146408886 countsByYear W21464088862019 @default.
• W2146408886 countsByYear W21464088862020 @default.
• W2146408886 countsByYear W21464088862021 @default.
• W2146408886 countsByYear W21464088862022 @default.
• W2146408886 countsByYear W21464088862023 @default.
• W2146408886 crossrefType "journal-article" @default.
• W2146408886 hasAuthorship W2146408886A5000656223 @default.
• W2146408886 hasAuthorship W2146408886A5028530566 @default.
• W2146408886 hasAuthorship W2146408886A5036028850 @default.
• W2146408886 hasConcept C104317684 @default.
• W2146408886 hasConcept C105580179 @default.
• W2146408886 hasConcept C150194340 @default.
• W2146408886 hasConcept C153911025 @default.
• W2146408886 hasConcept C177707886 @default.
• W2146408886 hasConcept C181199279 @default.
• W2146408886 hasConcept C187882448 @default.
• W2146408886 hasConcept C194583182 @default.
• W2146408886 hasConcept C2776551241 @default.
• W2146408886 hasConcept C36823959 @default.
• W2146408886 hasConcept C55493867 @default.
• W2146408886 hasConcept C86803240 @default.
• W2146408886 hasConceptScore W2146408886C104317684 @default.
• W2146408886 hasConceptScore W2146408886C105580179 @default.
• W2146408886 hasConceptScore W2146408886C150194340 @default.
• W2146408886 hasConceptScore W2146408886C153911025 @default.
• W2146408886 hasConceptScore W2146408886C177707886 @default.
• W2146408886 hasConceptScore W2146408886C181199279 @default.
• W2146408886 hasConceptScore W2146408886C187882448 @default.

• next