FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2146432584> ?p ?o ?g. }

• W2146432584 endingPage "H1183" @default.
• W2146432584 startingPage "H1175" @default.
• W2146432584 abstract "Heat shock protein 70 (Hsp70) can physically interact with and prevent thermal inactivation of sarco(endo)plasmic reticulum Ca 2+ -ATPase (SERCA) 1a, the SERCA isoform expressed in adult fast-twitch skeletal muscle. This study examined whether Hsp70 could physically interact with and prevent thermal inactivation of SERCA2a, the SERCA isoform expressed in heart. HEK-293 cells were cotransfected with cDNAs encoding human Hsp70 and rabbit SERCA2a (S2a/Hsp70). Cells cotransfected with SERCA2a cDNA and pMT2 (S2a/pMT2) were used as control. One-half of the cells was heat shocked at 40°C for 1 h (HS), and one-half was maintained at 37°C before harvesting the cells and isolating microsomes. Western blot analysis showed that Hsp70 and SERCA2a were colocalized in the microsomal fraction. The levels of Hsp70 were approximately fivefold higher ( P < 0.05) in S2a/Hsp70 compared with S2a/pMT2 and approximately twofold higher ( P < 0.05) following HS in all cells. Coimmunoprecipitation demonstrated that Hsp70 directly binds to SERCA2a. Following HS, maximal SERCA2a activity was reduced (∼52%, P < 0.05) in S2a/pMT2 but was increased (∼33%, P < 0.05) in S2a/Hsp70. Thermal inactivation of SERCA2a in S2a/pMT2 was associated with decreased (∼49%, P < 0.05) binding capacity for fluorescein isothiocyanate (FITC) and increased carbonyl (∼42%, P < 0.05) and nitrotyrosine (∼40%, P < 0.05) levels in SERCA2a. By contrast, the HS-induced increase in maximal SERCA2a activity observed in S2a/Hsp70 corresponded with no change ( P > 0.05) in FITC-binding capacity and reductions in carbonyl (∼40%, P < 0.05) and nitrotyrosine (∼23%, P < 0.05) levels in SERCA2a compared with S2a/pMT2. These results show that Hsp70 forms a protective interaction with SERCA2a during HS actually reducing oxidation and nitrosylation of SERCA2a thus increasing its maximal activity." @default.
• W2146432584 created "2016-06-24" @default.
• W2146432584 creator A5041040281 @default.
• W2146432584 creator A5047788840 @default.
• W2146432584 date "2009-04-01" @default.
• W2146432584 modified "2023-10-13" @default.
• W2146432584 title "Protective effects of Hsp70 on the structure and function of SERCA2a expressed in HEK-293 cells during heat stress" @default.
• W2146432584 cites W176044681 @default.
• W2146432584 cites W1760671874 @default.
• W2146432584 cites W1769448430 @default.
• W2146432584 cites W1823377985 @default.
• W2146432584 cites W184935174 @default.
• W2146432584 cites W1892307949 @default.
• W2146432584 cites W1963770237 @default.
• W2146432584 cites W1966502113 @default.
• W2146432584 cites W1972761725 @default.
• W2146432584 cites W1974836869 @default.
• W2146432584 cites W1978537703 @default.
• W2146432584 cites W1982260690 @default.
• W2146432584 cites W1997605861 @default.
• W2146432584 cites W2006659891 @default.
• W2146432584 cites W2007728507 @default.
• W2146432584 cites W2009346539 @default.
• W2146432584 cites W2009506745 @default.
• W2146432584 cites W2010576268 @default.
• W2146432584 cites W2010673396 @default.
• W2146432584 cites W2013578935 @default.
• W2146432584 cites W2016704244 @default.
• W2146432584 cites W2020988577 @default.
• W2146432584 cites W2023032045 @default.
• W2146432584 cites W2033432718 @default.
• W2146432584 cites W2037922556 @default.
• W2146432584 cites W2039388361 @default.
• W2146432584 cites W2044500356 @default.
• W2146432584 cites W2050538698 @default.
• W2146432584 cites W2051818097 @default.
• W2146432584 cites W2061688246 @default.
• W2146432584 cites W2061953115 @default.
• W2146432584 cites W2062641828 @default.
• W2146432584 cites W2063664389 @default.
• W2146432584 cites W2073718472 @default.
• W2146432584 cites W2078396761 @default.
• W2146432584 cites W2082668576 @default.
• W2146432584 cites W2086131676 @default.
• W2146432584 cites W2092175923 @default.
• W2146432584 cites W2092279282 @default.
• W2146432584 cites W2100837269 @default.
• W2146432584 cites W2103647634 @default.
• W2146432584 cites W2104601678 @default.
• W2146432584 cites W2105835713 @default.
• W2146432584 cites W2108786117 @default.
• W2146432584 cites W2115362310 @default.
• W2146432584 cites W2124980231 @default.
• W2146432584 cites W2131596778 @default.
• W2146432584 cites W2148823498 @default.
• W2146432584 cites W2149180473 @default.
• W2146432584 cites W2150608920 @default.
• W2146432584 cites W2153331570 @default.
• W2146432584 cites W2154584172 @default.
• W2146432584 cites W2183724114 @default.
• W2146432584 cites W2264443444 @default.
• W2146432584 cites W2312389273 @default.
• W2146432584 cites W4247441327 @default.
• W2146432584 cites W561931479 @default.
• W2146432584 doi "https://doi.org/10.1152/ajpheart.01276.2008" @default.
• W2146432584 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19252085" @default.
• W2146432584 hasPublicationYear "2009" @default.
• W2146432584 type Work @default.
• W2146432584 sameAs 2146432584 @default.
• W2146432584 citedByCount "44" @default.
• W2146432584 countsByYear W21464325842012 @default.
• W2146432584 countsByYear W21464325842013 @default.
• W2146432584 countsByYear W21464325842014 @default.
• W2146432584 countsByYear W21464325842015 @default.
• W2146432584 countsByYear W21464325842016 @default.
• W2146432584 countsByYear W21464325842017 @default.
• W2146432584 countsByYear W21464325842018 @default.
• W2146432584 countsByYear W21464325842019 @default.
• W2146432584 countsByYear W21464325842020 @default.
• W2146432584 countsByYear W21464325842021 @default.
• W2146432584 countsByYear W21464325842022 @default.
• W2146432584 countsByYear W21464325842023 @default.
• W2146432584 crossrefType "journal-article" @default.
• W2146432584 hasAuthorship W2146432584A5041040281 @default.
• W2146432584 hasAuthorship W2146432584A5047788840 @default.
• W2146432584 hasConcept C104317684 @default.
• W2146432584 hasConcept C126322002 @default.
• W2146432584 hasConcept C134018914 @default.
• W2146432584 hasConcept C153911025 @default.
• W2146432584 hasConcept C181199279 @default.
• W2146432584 hasConcept C185592680 @default.
• W2146432584 hasConcept C205260736 @default.
• W2146432584 hasConcept C23265538 @default.
• W2146432584 hasConcept C2776415932 @default.
• W2146432584 hasConcept C2781414619 @default.
• W2146432584 hasConcept C53345823 @default.
• W2146432584 hasConcept C55493867 @default.
• W2146432584 hasConcept C68991219 @default.

• next