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• W2146463722 abstract "OBJECTIVE: Safety and molecular mechanism of Four-Agents-Decoction (FADD, or Si-Wu-Tang, SWT: water extracts of Si-Wu), a traditional Chinese herb formula, on endometriosis. (Dysmenorrhea is common in female of reproductive age. Among those dysmenorrheic women, some dysmenorrhea are associated with pelvic diseases such as endometriosis, adenomyosis or uterine myoma. Endometriosis is an important cause of persistent dysmenorrhea. Regular use of FADD to treat dysmenorrhea might unintentionally expose endometriosis to FADD. The safety of regular use of FADD to treat dysmenorrhea and its molecular mechanism is investigated.).DESIGN: This is a perspective study, conducted in a medical school based medical center.MATERIALS AND METHODS: The endometriotic tissues were obtained from ovarian endometrioma after washed clean in PBS buffer. They were then cut into 5mm square and then culcured in DMEM/F12 with 10% fetal calf serum. aerated with 5% CO2. They were then treated with 1mg/ml or 10mg/ml of FADD for 24 and 48 hours. Then the protein from the treated tissue was obtained for western blot analysis. Western blot analysis with antibody to HER2,EGFR,Akt,ERK,VEGF and COX-2 were done. In addition, the same experiment was conducted in two ovarian cancer cell lines, SKOV-3 and TOV-21G. To explorer the mechanism of FADD effect on HER2 protein expression; HER2 reporter assay was preceded.RESULTS: Human epidermal growth factor receptor 2 (HER2) is cyclically expressed in endometrium at different menstrual phases. In this study, we demonstrated that FADD reduced HER2 protein expression (50%) in cultured human ovarian endometriotic tissues. In addition, the HER2-downstream target of phospho-Akt (serine-threonine kinase), activated form of Akt, was also downregulated by FADD treatment. HER2 overexpression is found in some female malignancies; breast cancer and some ovarian cancers. The above results were also shown by treatment of FADD on ovarian cancer cells, SKOV-3 and TOV-21G. HER2 promoter activities are suppressed by FADD in a dose-dependent manner. Thus, FADD may downregulate HER2 promoter activities to reduce HER2 protein expression. Taken together, FADD can downregulate HER2 and its downstream phospho-Akt protein levels through suppression of HER2 promoter activities.CONCLUSIONS: FADD can downregulate HER2 and its downstream phospho-Akt protein levels through suppression of HER2 promoter activities in endometrioma, and might endow some beneficial effect on endometriosis therapy. OBJECTIVE: Safety and molecular mechanism of Four-Agents-Decoction (FADD, or Si-Wu-Tang, SWT: water extracts of Si-Wu), a traditional Chinese herb formula, on endometriosis. (Dysmenorrhea is common in female of reproductive age. Among those dysmenorrheic women, some dysmenorrhea are associated with pelvic diseases such as endometriosis, adenomyosis or uterine myoma. Endometriosis is an important cause of persistent dysmenorrhea. Regular use of FADD to treat dysmenorrhea might unintentionally expose endometriosis to FADD. The safety of regular use of FADD to treat dysmenorrhea and its molecular mechanism is investigated.). DESIGN: This is a perspective study, conducted in a medical school based medical center. MATERIALS AND METHODS: The endometriotic tissues were obtained from ovarian endometrioma after washed clean in PBS buffer. They were then cut into 5mm square and then culcured in DMEM/F12 with 10% fetal calf serum. aerated with 5% CO2. They were then treated with 1mg/ml or 10mg/ml of FADD for 24 and 48 hours. Then the protein from the treated tissue was obtained for western blot analysis. Western blot analysis with antibody to HER2,EGFR,Akt,ERK,VEGF and COX-2 were done. In addition, the same experiment was conducted in two ovarian cancer cell lines, SKOV-3 and TOV-21G. To explorer the mechanism of FADD effect on HER2 protein expression; HER2 reporter assay was preceded. RESULTS: Human epidermal growth factor receptor 2 (HER2) is cyclically expressed in endometrium at different menstrual phases. In this study, we demonstrated that FADD reduced HER2 protein expression (50%) in cultured human ovarian endometriotic tissues. In addition, the HER2-downstream target of phospho-Akt (serine-threonine kinase), activated form of Akt, was also downregulated by FADD treatment. HER2 overexpression is found in some female malignancies; breast cancer and some ovarian cancers. The above results were also shown by treatment of FADD on ovarian cancer cells, SKOV-3 and TOV-21G. HER2 promoter activities are suppressed by FADD in a dose-dependent manner. Thus, FADD may downregulate HER2 promoter activities to reduce HER2 protein expression. Taken together, FADD can downregulate HER2 and its downstream phospho-Akt protein levels through suppression of HER2 promoter activities. CONCLUSIONS: FADD can downregulate HER2 and its downstream phospho-Akt protein levels through suppression of HER2 promoter activities in endometrioma, and might endow some beneficial effect on endometriosis therapy." @default.
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• W2146463722 date "2008-09-01" @default.
• W2146463722 modified "2023-09-26" @default.
• W2146463722 title "Four-agents-decoction suppresses HER2 expression in endometriosis" @default.
• W2146463722 doi "https://doi.org/10.1016/j.fertnstert.2008.07.254" @default.
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