FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2146556384> ?p ?o ?g. }

• W2146556384 abstract "The chitin synthase that makes the primary septum during cell division in budding yeasts is an important therapeutic target with an unknown activation mechanism. We previously found that the C2-domain of the Saccharomyces cerevisiae Inn1 protein plays an essential but uncharacterised role at the cleavage site during cytokinesis. By combining a novel degron allele of INN1 with a point mutation in the C2-domain, we screened for mutations in other genes that suppress the resulting defect in cell division. In this way we identified 22 dominant mutations of CHS2 (Chitin Synthase II) that map to two neighbouring sites in the catalytic domain. Whereas Chs2 in isolated cell membranes is normally almost inactive, unless protease treatment is used to bypass inhibition, the dominant suppressor allele Chs2-V377I has enhanced activity in vitro. We show that Inn1 associates with Chs2 in yeast cell extracts, and interacts in the yeast two-hybrid assay with the amino-terminal 65% of Chs2 that contains the catalytic domain. In addition to compensating for mutations in the Inn1 C2-domain, the dominant CHS2 alleles also suppress cytokinesis defects produced by lack of the Cyk3 protein, and our data support a model whereby the C2-domain of Inn1 acts in conjunction with Cyk3 to regulate the catalytic domain of Chs2 during cytokinesis. These findings suggest novel approaches for developing future drugs against important fungal pathogens." @default.
• W2146556384 created "2016-06-24" @default.
• W2146556384 creator A5006884115 @default.
• W2146556384 creator A5011816069 @default.
• W2146556384 creator A5028709855 @default.
• W2146556384 creator A5048930087 @default.
• W2146556384 creator A5073735040 @default.
• W2146556384 date "2012-01-01" @default.
• W2146556384 modified "2023-10-16" @default.
• W2146556384 title "Inn1 and Cyk3 regulate chitin synthase during cytokinesis in budding yeasts" @default.
• W2146556384 cites W1566200502 @default.
• W2146556384 cites W1585133178 @default.
• W2146556384 cites W1869411311 @default.
• W2146556384 cites W1965813899 @default.
• W2146556384 cites W1968074226 @default.
• W2146556384 cites W1976952458 @default.
• W2146556384 cites W1983321353 @default.
• W2146556384 cites W1984173824 @default.
• W2146556384 cites W1993613706 @default.
• W2146556384 cites W1994788479 @default.
• W2146556384 cites W1998143618 @default.
• W2146556384 cites W2000309853 @default.
• W2146556384 cites W2002058224 @default.
• W2146556384 cites W2006831634 @default.
• W2146556384 cites W2021804769 @default.
• W2146556384 cites W2025823358 @default.
• W2146556384 cites W2031261777 @default.
• W2146556384 cites W2031647687 @default.
• W2146556384 cites W2036665353 @default.
• W2146556384 cites W2041292476 @default.
• W2146556384 cites W2041859788 @default.
• W2146556384 cites W2048599311 @default.
• W2146556384 cites W2049197795 @default.
• W2146556384 cites W2052138090 @default.
• W2146556384 cites W2052767634 @default.
• W2146556384 cites W2053218152 @default.
• W2146556384 cites W2056573634 @default.
• W2146556384 cites W2059966388 @default.
• W2146556384 cites W2060259273 @default.
• W2146556384 cites W2060989256 @default.
• W2146556384 cites W2062741934 @default.
• W2146556384 cites W2063271557 @default.
• W2146556384 cites W2065255935 @default.
• W2146556384 cites W2069635419 @default.
• W2146556384 cites W2072536047 @default.
• W2146556384 cites W2078190331 @default.
• W2146556384 cites W2082507962 @default.
• W2146556384 cites W2098715685 @default.
• W2146556384 cites W2116549724 @default.
• W2146556384 cites W2118686778 @default.
• W2146556384 cites W2122548085 @default.
• W2146556384 cites W2123825436 @default.
• W2146556384 cites W2125680927 @default.
• W2146556384 cites W2125844053 @default.
• W2146556384 cites W2126736493 @default.
• W2146556384 cites W2143138666 @default.
• W2146556384 cites W2146138612 @default.
• W2146556384 cites W2154206349 @default.
• W2146556384 cites W2156809804 @default.
• W2146556384 cites W2164850267 @default.
• W2146556384 cites W2169333620 @default.
• W2146556384 cites W2298630481 @default.
• W2146556384 cites W4252538912 @default.
• W2146556384 doi "https://doi.org/10.1242/jcs.109157" @default.
• W2146556384 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22956544" @default.
• W2146556384 hasPublicationYear "2012" @default.
• W2146556384 type Work @default.
• W2146556384 sameAs 2146556384 @default.
• W2146556384 citedByCount "39" @default.
• W2146556384 countsByYear W21465563842013 @default.
• W2146556384 countsByYear W21465563842014 @default.
• W2146556384 countsByYear W21465563842015 @default.
• W2146556384 countsByYear W21465563842016 @default.
• W2146556384 countsByYear W21465563842017 @default.
• W2146556384 countsByYear W21465563842018 @default.
• W2146556384 countsByYear W21465563842019 @default.
• W2146556384 countsByYear W21465563842020 @default.
• W2146556384 countsByYear W21465563842021 @default.
• W2146556384 countsByYear W21465563842022 @default.
• W2146556384 countsByYear W21465563842023 @default.
• W2146556384 crossrefType "journal-article" @default.
• W2146556384 hasAuthorship W2146556384A5006884115 @default.
• W2146556384 hasAuthorship W2146556384A5011816069 @default.
• W2146556384 hasAuthorship W2146556384A5028709855 @default.
• W2146556384 hasAuthorship W2146556384A5048930087 @default.
• W2146556384 hasAuthorship W2146556384A5073735040 @default.
• W2146556384 hasBestOaLocation W21465563841 @default.
• W2146556384 hasConcept C1491633281 @default.
• W2146556384 hasConcept C179411191 @default.
• W2146556384 hasConcept C2777576037 @default.
• W2146556384 hasConcept C2779222958 @default.
• W2146556384 hasConcept C2779732960 @default.
• W2146556384 hasConcept C2780992308 @default.
• W2146556384 hasConcept C2781088250 @default.
• W2146556384 hasConcept C45472230 @default.
• W2146556384 hasConcept C54355233 @default.
• W2146556384 hasConcept C55493867 @default.
• W2146556384 hasConcept C85813293 @default.
• W2146556384 hasConcept C86803240 @default.
• W2146556384 hasConcept C95444343 @default.

• next