Matches in SemOpenAlex for { <https://semopenalex.org/work/W2146588715> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2146588715 endingPage "190" @default.
- W2146588715 startingPage "183" @default.
- W2146588715 abstract "We describe an ex vivo rat infection model to study protective immunity against Fasciola hepatica at the gut level. An exact number of newly excysted juveniles (NEJs) was injected into a gut segment with an intact blood supply and which was still attached to a live anaesthetized rat. NEJs that penetrated the gut wall during the following 6 h were recovered from a beaker filled with medium and were counted under a microscope. This infection model was validated and enabled us to exactly quantify the infection dose whilst at the same time exactly quantifying the number of NEJs penetrating the gut wall. The mean sum of NEJs that migrated through the gut wall into the beaker (peritoneal fraction), plus NEJs that remained in the gut wall and the gut lumen was 87% of the infective dose (±3.6% SEM; n=18). The function of the ex vivo segments was well-preserved, as demonstrated by only minor leakage of an inert liquid marker. The ex vivo model enabled us to measure protection against F. hepatica at the gut level. In naive rats 52% (±2.4% SEM; n=40) of the injected NEJs penetrated the gut wall, whereas in previously infected rats only 12% (±1.8% SEM; n=40) were able to do so, irrespective of the infection dose. Thus, when rats were orally primed, the migration of NEJs through the gut wall was 77% less than the migration in naive rats. We conclude that the ex vivo model should be valuable in studies of the induction and expression of protective immunity against F. hepatica in the intestine, and will aid in development and optimization of vaccines." @default.
- W2146588715 created "2016-06-24" @default.
- W2146588715 creator A5036202799 @default.
- W2146588715 creator A5040664181 @default.
- W2146588715 creator A5045488978 @default.
- W2146588715 creator A5067068508 @default.
- W2146588715 date "1998-06-01" @default.
- W2146588715 modified "2023-10-13" @default.
- W2146588715 title "A novel ex vivo rat infection model to study protective immunity against Fasciola hepatica at the gut level" @default.
- W2146588715 cites W1989888749 @default.
- W2146588715 cites W2002381929 @default.
- W2146588715 cites W2007065369 @default.
- W2146588715 cites W2037700122 @default.
- W2146588715 cites W2049346825 @default.
- W2146588715 cites W2051578927 @default.
- W2146588715 cites W2058217983 @default.
- W2146588715 cites W2063012394 @default.
- W2146588715 cites W2081000503 @default.
- W2146588715 cites W2085428387 @default.
- W2146588715 cites W2086345891 @default.
- W2146588715 cites W2092034339 @default.
- W2146588715 cites W2157157836 @default.
- W2146588715 cites W2166421314 @default.
- W2146588715 cites W2329202239 @default.
- W2146588715 cites W2405685847 @default.
- W2146588715 cites W2415967451 @default.
- W2146588715 cites W2417748389 @default.
- W2146588715 cites W2433406271 @default.
- W2146588715 cites W2467367863 @default.
- W2146588715 cites W2773032584 @default.
- W2146588715 cites W4297987445 @default.
- W2146588715 cites W4302370177 @default.
- W2146588715 cites W50277004 @default.
- W2146588715 cites W2409295390 @default.
- W2146588715 doi "https://doi.org/10.1016/s0022-1759(98)00026-x" @default.
- W2146588715 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9692850" @default.
- W2146588715 hasPublicationYear "1998" @default.
- W2146588715 type Work @default.
- W2146588715 sameAs 2146588715 @default.
- W2146588715 citedByCount "19" @default.
- W2146588715 countsByYear W21465887152012 @default.
- W2146588715 countsByYear W21465887152013 @default.
- W2146588715 countsByYear W21465887152015 @default.
- W2146588715 countsByYear W21465887152016 @default.
- W2146588715 countsByYear W21465887152020 @default.
- W2146588715 countsByYear W21465887152021 @default.
- W2146588715 countsByYear W21465887152022 @default.
- W2146588715 countsByYear W21465887152023 @default.
- W2146588715 crossrefType "journal-article" @default.
- W2146588715 hasAuthorship W2146588715A5036202799 @default.
- W2146588715 hasAuthorship W2146588715A5040664181 @default.
- W2146588715 hasAuthorship W2146588715A5045488978 @default.
- W2146588715 hasAuthorship W2146588715A5067068508 @default.
- W2146588715 hasConcept C131631996 @default.
- W2146588715 hasConcept C150903083 @default.
- W2146588715 hasConcept C165901193 @default.
- W2146588715 hasConcept C203014093 @default.
- W2146588715 hasConcept C207001950 @default.
- W2146588715 hasConcept C26291073 @default.
- W2146588715 hasConcept C2777856171 @default.
- W2146588715 hasConcept C2779341262 @default.
- W2146588715 hasConcept C86803240 @default.
- W2146588715 hasConcept C8891405 @default.
- W2146588715 hasConcept C95444343 @default.
- W2146588715 hasConceptScore W2146588715C131631996 @default.
- W2146588715 hasConceptScore W2146588715C150903083 @default.
- W2146588715 hasConceptScore W2146588715C165901193 @default.
- W2146588715 hasConceptScore W2146588715C203014093 @default.
- W2146588715 hasConceptScore W2146588715C207001950 @default.
- W2146588715 hasConceptScore W2146588715C26291073 @default.
- W2146588715 hasConceptScore W2146588715C2777856171 @default.
- W2146588715 hasConceptScore W2146588715C2779341262 @default.
- W2146588715 hasConceptScore W2146588715C86803240 @default.
- W2146588715 hasConceptScore W2146588715C8891405 @default.
- W2146588715 hasConceptScore W2146588715C95444343 @default.
- W2146588715 hasIssue "2" @default.
- W2146588715 hasLocation W21465887151 @default.
- W2146588715 hasLocation W21465887152 @default.
- W2146588715 hasOpenAccess W2146588715 @default.
- W2146588715 hasPrimaryLocation W21465887151 @default.
- W2146588715 hasRelatedWork W1975498499 @default.
- W2146588715 hasRelatedWork W2037182962 @default.
- W2146588715 hasRelatedWork W2037700122 @default.
- W2146588715 hasRelatedWork W2074470970 @default.
- W2146588715 hasRelatedWork W2077820532 @default.
- W2146588715 hasRelatedWork W2140910281 @default.
- W2146588715 hasRelatedWork W2329677975 @default.
- W2146588715 hasRelatedWork W2554934382 @default.
- W2146588715 hasRelatedWork W2979791217 @default.
- W2146588715 hasRelatedWork W2185621195 @default.
- W2146588715 hasVolume "213" @default.
- W2146588715 isParatext "false" @default.
- W2146588715 isRetracted "false" @default.
- W2146588715 magId "2146588715" @default.
- W2146588715 workType "article" @default.